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ELISA Buffer Kit
ELISA Buffer Kit
Zitierungen und Referenzen (2)
Invitrogen™

ELISA Buffer Kit

Das ELISA-Puffer-Kit enthält alle grundlegenden Puffer, die für die Verwendung von Invitrogen-Antikörperpaaren oder für die Ausführung eines ELISA erforderlich sind.Weitere Informationen
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KatalognummerMenge
CNB001110 Platten
Katalognummer CNB0011
Preis (EUR)
300,65
Exklusiv online
320,00
Ersparnis 19,35 (6%)
Each
Zum Warenkorb hinzufügen
Menge:
10 Platten
Preis (EUR)
300,65
Exklusiv online
320,00
Ersparnis 19,35 (6%)
Each
Zum Warenkorb hinzufügen
Das ELISA-Puffer-Kit enthält alle grundlegenden Puffer, die für die Verwendung von Invitrogen-Antikörperpaaren oder für die Ausführung eines ELISA erforderlich sind. Das Kit enthält ausreichend Material für zehn ELISA-Platten.

Inhalt des Kits:
• Beschichtungspuffer A (10 mM Phosphatpuffer, pH-Wert 7,4)
• Beschichtungspuffer B (50 mM Carbonatpuffer, pH-Wert 9,4)
• Assay-Puffer (Blockierungspuffer) (5x)
• Waschpuffer (25x)
• Stabilisiertes TMB
• Stopplösung

Alle verfügbaren Puffer und Reagenzien für ELISA-Anwendungen anzeigen.

Nur für Forschungszwecke. Darf nicht für diagnostische Verfahren eingesetzt werden.
Specifications
Zur Verwendung mit (Anwendung)ELISA
FormKonzentrat
VersandbedingungNasseis
Menge10 Platten
Unit SizeEach
Inhalt und Lagerung
Beschichtungspuffer A (100 ml): 1 Flasche
Beschichtungspuffer B (100 ml): 1 Flasche
Assay-Puffer (5x) (200 ml): 1 Flasche
Waschpuffer (25x) (100 ml): 3 Flaschen
Chromogen, stabilisiert (25 ml): 4 Flaschen
Stopplösung (100 ml): 1 Flasche. Bei 2 °C bis 8 °C lagern.

Häufig gestellte Fragen (FAQ)

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Zitierungen und Referenzen (2)

Zitierungen und Referenzen
Abstract
Expression of innate immune response genes in liver and three types of adipose tissue in cloned pigs.
Authors:Rødgaard T, Skovgaard K, Stagsted J, Heegaard PM,
Journal:Cell Reprogram
PubMed ID:22928970
The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by the cloning process. ... More
Mechanisms of toxicity induced by SiO2 nanoparticles of in vitro human alveolar barrier: effects on cytokine production, oxidative stress induction, surfactant proteins A mRNA expression and nanoparticles uptake.
Authors:Farcal LR, Uboldi C, Mehn D, Giudetti G, Nativo P, Ponti J, Gilliland D, Rossi F, Bal-Price A,
Journal:Nanotoxicology
PubMed ID:22769972
'Abstract An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS1 was used to evaluate the effects of amorphous silicon dioxide nanoparticles (SiNPs), in the presence or absence of THP-1 cells (monocytes). SiNPs exposure induced production of proinflammatory ... More