ELISA Buffer Kit
ELISA Buffer Kit
Citas y referencias (2)
Invitrogen™

ELISA Buffer Kit

El kit de tampón ELISA contiene todos los tampones básicos necesarios para el uso de pares de anticuerpos Invitrogen oMás información
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Número de catálogoCantidad
CNB001110 placas
Número de catálogo CNB0011
Precio (MXN)
-
Cantidad:
10 placas
El kit de tampón ELISA contiene todos los tampones básicos necesarios para el uso de pares de anticuerpos Invitrogen o que son necesarios para ejecutar un ELISA. El kit proporciona material suficiente para 10 placas ELISA.

Componentes del kit:
• Tampón de revestimiento A (tampón de fosfato 10 mM, pH 7,4)
• Tampón de revestimiento B (tampón de carbonato 50 mM, pH 9,4)
• Tampón de ensayo (tampón de bloqueo) (5X)
• Tampón de lavado (25X)
• TMB estabilizado
• Solución de parada

Ver todos los tampones y reactivos disponibles para aplicaciones ELISA.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (aplicación)ELISA
FormularioConcentración
Condiciones de envíoHielo húmedo
Cantidad10 placas
Unit SizeEach
Contenido y almacenamiento
Tampón de recubrimiento A (100 ml): 1 frasco
de tampón de recubrimiento B (100 ml): 1 frasco
de tampón de ensayo de (5x) (200 ml): 1 frasco
de tampón de lavado (25x) (100 ml): 3 frascos
de cromógeno estabilizado (25 ml): 4 frascos
de solución de parada (100 ml): 1 frasco. Almacenar entre 2 °C y 8 °C.

Preguntas frecuentes

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Citations & References (2)

Citations & References
Abstract
Expression of innate immune response genes in liver and three types of adipose tissue in cloned pigs.
Authors:Rødgaard T, Skovgaard K, Stagsted J, Heegaard PM,
Journal:Cell Reprogram
PubMed ID:22928970
The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by the cloning process. ... More
Mechanisms of toxicity induced by SiO2 nanoparticles of in vitro human alveolar barrier: effects on cytokine production, oxidative stress induction, surfactant proteins A mRNA expression and nanoparticles uptake.
Authors:Farcal LR, Uboldi C, Mehn D, Giudetti G, Nativo P, Ponti J, Gilliland D, Rossi F, Bal-Price A,
Journal:Nanotoxicology
PubMed ID:22769972
'Abstract An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS1 was used to evaluate the effects of amorphous silicon dioxide nanoparticles (SiNPs), in the presence or absence of THP-1 cells (monocytes). SiNPs exposure induced production of proinflammatory ... More