Kit de soluciones iBind™
Kit de soluciones iBind™
Invitrogen™

Kit de soluciones iBind™

El kit de soluciones iBind se utiliza con el dispositivo iBind Western que es un dispositivo de procesamiento de WesternMás información
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Número de catálogoCantidad
SLF10201 Kit
SLF1020X4Suficiente para 10 reacciones de minimembrana
Número de catálogo SLF1020
Precio (CLP)
117.847
1 kit
Añadir al carro de la compra
Cantidad:
1 Kit
Precio (CLP)
117.847
1 kit
Añadir al carro de la compra
El kit de soluciones iBind se utiliza con el dispositivo iBind Western que es un dispositivo de procesamiento de Western automatizado que realiza todos los pasos del flujo de trabajo de Western, desde el bloqueo y el lavado hasta la incubación de anticuerpos. Las soluciones iBind actúan como soluciones de bloqueo, lavado y dilución en el proceso de inmunodetección iBind y se han optimizado para tener una viscosidad ideal. Cada solución pasa de los pocillos del dispositivo iBind a la tarjeta iBind mediante el flujo lateral secuencial (SLF); seguidamente, las soluciones se transfieren hacia la zona de la pila de la tarjeta. La matriz de fibra de vidrio de la tarjeta permite el flujo uniforme y homogéneo de las soluciones hacia la membrana, lo que aumenta la interacción entre el antígeno y el anticuerpo.

Obtenga más información sobre los sistemas iBind Western›

El kit de soluciones de iBind, junto con el dispositivo iBind Western y las tarjetas iBind, aportan automatización y simplicidad al protocolo occidental. Cada kit de soluciones iBind contiene suficiente solución para 10 reacciones de minimembrana. Para usar este kit es necesario un dispositivo iBind Western. Para aquellas aplicaciones que emplean métodos fluorescentes, utilice el kit de solución de detección fluorescente (FD) iBind.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
N.º de reacciones10 reacciones de minimembrana
Cantidad1 Kit
Tipo de muestraproteína
Método de detecciónColorimétrico, cromogénico, quimioluminiscente
Para utilizar con (aplicación)Inmunotransferencia (Western Blotting)
Para utilizar con (equipo)Dispositivo iBind Western
Membrane CompatibilityNitrocelulosa, PVDF
Línea de productosiBind
TipoKit de soluciones
Unit Size1 kit
Contenido y almacenamiento
Almacenar a 2–4 °C.

Preguntas frecuentes

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What iBind Solution kits are available?

We offer the iBind Solution kit and the iBind Fluorescent Detection (FD) Solution kit. For standard chemiluminescent or chromogenic detection, we recommend the iBind Solution kit (Cat. No. SLF1020) and for fluorescent detection, we recommend the iBind Fluorescent Detection (FD) Solution kit (Cat. No. SLF1019).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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