Zitierungen und Referenzen (6)

Gibco™

PBS, pH 7,4

Name: PBS, pH 7,4
SKU: 10010023
Price: 35.65 EUR

PBS (phosphatgepufferte Salzlösung) ist eine ausgeglichene Salzlösung, die bei einer Vielzahl von Zellkulturanwendungen zum Einsatz kommt. Dazu gehören das WaschenWeitere Informationen

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Katalognummer	Menge
10010023	500 mL
10010031	1.000 mL

2 Optionen

Katalognummer 10010023

Preis (EUR)

35,65

キャンペーン価格

38,37

Ersparnis 2,72 (7%)

Each

Menge:

500 mL

Preis (EUR)

35,65

キャンペーン価格

38,37

Ersparnis 2,72 (7%)

Each

PBS (phosphatgepufferte Salzlösung) ist eine ausgeglichene Salzlösung, die bei einer Vielzahl von Zellkulturanwendungen zum Einsatz kommt. Dazu gehören das Waschen von Zellen vor der Dissoziation, der Transport von Zellen und Gewebe, das Verdünnen von Zellen für Zellzählungen und die Vorbereitung von Reagenzien. Damit das Ausspülen von Chelatoren aus der Kultur vor der Zelldissoziation möglich wird, enthält PBS kein Calcium und Magnesium. Wir bieten eine Vielzahl von PBS-Formulierungen für verschiedene Zellkulturanwendungen an. Finden Sie die richtige Zusammensetzung mithilfe des Medien-Auswahlwerkzeuges.

Diese PBS wurde wie folgt hergestellt:

Ohne

• Calcium

• Magnesium

• Phenolrot

Die vollständige Formulierung ist verfügbar.

cGMP-konformes Fertigungs- und Qualitätssystem
PBS wird in einer cGMP-konformen Einrichtung in Paisley, Schottland (Großbritannien), hergestellt. Die Einrichtung ist von der US-Arzneimittelbehörde FDA als Hersteller von Medizinprodukten zugelassen und nach ISO 13485-zertifiziert. Um eine kontinuierliche Versorgung zu gewährleisten, bieten wir ein identisches PBS-Produkt an, dass an unserem Standort in Grand Island (USA) hergestellt wird (20012-050). Diese Einrichtung ist ebenfalls von der US-Arzneimittelbehörde FDA als Hersteller von Medizinprodukten zugelassen und nach ISO 13485-zertifiziert.

Für die Verwendung in der Forschung oder die Weiterverarbeitung. Nicht zur Diagnosestellung oder direkten Anwendung bei Menschen oder Tieren geeignet.

Specifications

Chemischer Name oder MaterialPhosphatgepufferte Salzlösung (PBS)

FarbeKlar

EnthältNo Calcium, No Magnesium

Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard

Osmolalität280 bis 315 mOsm/kg

VerpackungBottle

Empfohlene LagerungLagerbedingungen: 15 °C bis 30 °C
Versandbedingungen: Haltbarkeit bei
Raumtemperatur: 24 Monate ab Herstellungsdatum

SterilitätSteril gefiltert

Konzentration1 X

Zur Verwendung mit (Anwendung)Waschen von Zellen vor der Dissoziation; Verdünnen von Zellen zur Zählung und Vorbereitung von Reagenzien; Transport von Zellen oder Gewebe

Physikalische FormFlüssig

ProduktlinieGibco

Menge500 mL

LösungstypPhosphatgepufferte Salzlösung

pH7,4

Unit SizeEach

Häufig gestellte Fragen (FAQ)

How can I make isolation buffer for cell isolation?

To prepare this buffer:

(1) Make up 100 mL of a stock solution of 0.5 M EDTA by dissolving 14.6 g EDTA in approximately 80 mL of ddH2O. Adjust the pH to 7.4, then adjust the volume to 100 mL. Autoclave and filter 25 mL aliquots through a sterilized filter into 4 sterile 50 mL tubes. Store one at 4 degrees C and the rest at -20 degrees C for future use.
(2) Make up a stock solution of 20% BSA by putting 5 g of BSA in a 50 mL tube and filling it with ddH2O up to 25 mL. Leave on a roller at room temperature until dissolved. Filter through a sterile 0.2 µm filter into a sterile 50 mL tube and adjust the volume to 30 mL with sterile H2O.
(3) Make the isolation buffer by taking a 500 mL bottle of 1x PBS, pH 7.4 without Ca2+ or Mg2+ (Cat. No. 10010023). Add 2.5 mL 20% BSA and 2 mL 0.5 M EDTA. Mix by turning the bottle end over end 10 times. Store at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My PBS, PH 7.4 (Cat. No. 10010072) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products tested for endotoxin levels?

Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products sterile and suitable for cell culture?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.

All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products DNase- and RNase-free?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Zitierungen und Referenzen (6)

Zitierungen und Referenzen

Abstract

A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.

Authors:Romito E,Battistella I,Plakhova V,Paplekaj A,Forastieri C,Toffolo E,Musio C,Conti L,Battaglioli E,Rusconi F

Journal:Journal of neuroscience methods

PubMed ID:39053772

Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells.

Authors: Ramana Kota V; Chandra Deepak; Srivastava Sanjay; Bhatnagar Aruni; Aggarwal Bharat B; Srivastava Satish K;

Journal:J Biol Chem

PubMed ID:12063254

'Abnormal vascular smooth muscle cell (VSMC) proliferation is a key feature of atherosclerosis and restenosis; however, the mechanisms regulating growth remain unclear. Herein we show that inhibition of the aldehyde-metabolizing enzyme aldose reductase (AR) inhibits NF-kappa B activation during restenosis of balloon-injured rat carotid arteries as well as VSMC proliferation ... More

Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells.

Authors: Kabos Peter; Kabosova Andrea; Neuman Toomas;

Journal:J Biol Chem

PubMed ID:11809764

Mammalian neural stem cells can develop into a variety of neuronal and glial cell types. This involves a highly coordinated process of differentiation in which the Notch signaling pathway and the system of helix-loop-helix (HLH) transcriptional regulators play a key role. By exercising control over proliferation, initiation of differentiation, neurite ... More

Cholesterol modulates the membrane binding and intracellular distribution of annexin 6.

Authors: de Diego IÃ±aki; Schwartz Felix; Siegfried Heide; Dauterstedt Paul; Heeren Joerg; Beisiegel Ulrike; Enrich Carlos; Grewal Thomas;

Journal:J Biol Chem

PubMed ID:12070178

Annexins are Ca(2+)- and phospholipid-binding proteins that are widely expressed in mammalian tissues and that bind to different cellular membranes. In recent years its role in membrane traffic has emerged as one of its predominant functions, but the regulation of its intracellular distribution still remains unclear. We demonstrated that annexin ... More

A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.

Authors: Kachman Maureen T; Wang Haixing; Schwartz Donald R; Cho Kathleen R; Lubman David M;

Journal:Anal Chem

PubMed ID:11985308

A two-dimensional liquid phase separation of proteins from whole cell lysates coupled on-line to an electrospray-ionization time-of-flight (ESI-TOF) mass spectrometer (MS) is used to map the protein content of ovarian surface epithelial cells (OSE) and an ovarian carcinoma-derived cell line (ES2). The two dimensions involve the use of liquid isoelectric ... More

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