Zitierungen und Referenzen (6)

Gibco™

Carbenicillin Disodium Salt

Carbenicillin Dinatriumsalz ist ein halbsynthetisches Penicillin-Antibiotikum mit einer Carboxyl- und einer Benzylgruppe. Es hat den gleichen Wirkmechanismus wie Ampicillin. EsWeitere Informationen

Katalognummer	Menge
10177012	5 g

Katalognummer 10177012

Preis (EUR)

892,00

Menge:

Preis (EUR)

892,00

Carbenicillin Dinatriumsalz ist ein halbsynthetisches Penicillin-Antibiotikum mit einer Carboxyl- und einer Benzylgruppe. Es hat den gleichen Wirkmechanismus wie Ampicillin. Es interferiert mit der Zellwandsynthese von gramnegativen Bakterien und zeigt gleichzeitig eine schwache Toxizität gegenüber pflanzlichem Gewebe. Gibco Carbenicillin dient als selektives Antibiotikum für resistente Agrobakterien und E. coli, in der Regel in einer Konzentration von 50 – 100 µg/ml. Das β-Lactamase-Gen (bla), das eine Resistenz gegen Ampicillin verleiht, verleiht ebenfalls eine Resistenz gegen Carbenicillin. Es wird im Vergleich zu Ampicillin durch Beta-Lactamase langsamer abgebaut und ist somit stabiler. Es kann das Wachstum von Satellitenkolonien während langfristiger Inkubationen verringern. Dieses Produkt wird als Pulver geliefert und sollte durch Auflösung in Wasser zu einer Stammlösung mit 50 – 100 mg/ml verarbeitet werden.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

FarbeWeiß bis cremefarben

Konzentration50 bis 100 μg/ml

ProduktlinieGibco

Menge5 g

Haltbarkeit36 Monate

VersandbedingungNasseis

FormPulver

ProdukttypAntibiotikum

SterilitätSteril

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen: 2 bis 8 °C
Versandbedingungen: Eis
Haltbarkeit: 36 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

Can ampicillin be used for selection of eukaryotic cells if put under control of a eukaryotic/viral promoter?

No. B-lactamase is targeted to specific linkages in the bacterial cell wall. Since eukaryotic cells lack a cell wall, ampicillin has no effect upon eukaryotic cells.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Zitierungen und Referenzen (6)

Zitierungen und Referenzen

Abstract

The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis.

Authors:Di Stefano B, Luo EC, Haggerty C, Aigner S, Charlton J, Brumbaugh J, Ji F, Rabano Jiménez I, Clowers KJ, Huebner AJ, Clement K, Lipchina I, de Kort MAC, Anselmo A, Pulice J, Gerli MFM, Gu H, Gygi SP, Sadreyev RI, Meissner A, Yeo GW, Hochedlinger K

Journal:Cell Stem Cell

PubMed ID:31588046

'Post-transcriptional mechanisms have the potential to influence complex changes in gene expression, yet their role in cell fate transitions remains largely unexplored. Here, we show that suppression of the RNA helicase DDX6 endows human and mouse primed embryonic stem cells (ESCs) with a differentiation-resistant, "hyper-pluripotent" state, which readily reprograms to ... More

Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling.

Authors:Brumbaugh J, Di Stefano B, Wang X, Borkent M, Forouzmand E, Clowers KJ, Ji F, Schwarz BA, Kalocsay M, Elledge SJ, Chen Y, Sadreyev RI, Gygi SP, Hu G, Shi Y, Hochedlinger K

Journal:Cell

PubMed ID:29249356

'Cell fate transitions involve rapid gene expression changes and global chromatin remodeling, yet the underlying regulatory pathways remain incompletely understood. Here, we identified the RNA-processing factor Nudt21 as a novel regulator of cell fate change using transcription-factor-induced reprogramming as a screening assay. Suppression of Nudt21 enhanced the generation of induced ... More

High-throughput, image-based screening of pooled genetic-variant libraries.

Authors:Emanuel G, Moffitt JR, Zhuang X

Journal:Nat Methods

PubMed ID:29083401

We report a high-throughput screening method that allows diverse genotypes and corresponding phenotypes to be imaged in individual cells. We achieve genotyping by introducing barcoded genetic variants into cells as pooled libraries and reading the barcodes out using massively multiplexed fluorescence in situ hybridization. To demonstrate the power of image-based ... More

Salmonella Persist in Activated Macrophages in T Cell-Sparse Granulomas but Are Contained by Surrounding CXCR3 Ligand-Positioned Th1 Cells.

Authors:Goldberg MF, Roeske EK, Ward LN, Pengo T, Dileepan T, Kotov DI, Jenkins MK

Journal:Immunity

PubMed ID:30552021

Salmonella enterica (Se) bacteria cause persistent intracellular infections while stimulating a robust interferon-?-producing CD4

ATP-Dependent Dynamic Protein Aggregation Regulates Bacterial Dormancy Depth Critical for Antibiotic Tolerance.

Authors:Pu Y, Li Y, Jin X, Tian T, Ma Q, Zhao Z, Lin SY, Chen Z, Li B, Yao G, Leake MC, Lo CJ, Bai F

Journal:Mol Cell

PubMed ID:30472191

Cell dormancy is a widespread mechanism used by bacteria to evade environmental threats, including antibiotics. Here we monitored bacterial antibiotic tolerance and regrowth at the single-cell level and found that each individual survival cell shows different "dormancy depth," which in return regulates the lag time for cell resuscitation after removal ... More

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