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Platinum&trade; <i>Taq</i> DNA-Polymerase High Fidelity
Zitierungen und Referenzen (4)
Invitrogen™

Platinum™ Taq DNA-Polymerase High Fidelity

Platinum™ Taq High-Fidelity DNA-Polymerase eignet sich ideal für Amplifikationen von DNA-Fragmenten, wenn hohe Ausbeuten und eine verlässliche Amplifikation erforderlich sind.Weitere Informationen
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KatalognummerAnzahl Reaktionen
11304029500 Reaktionen
11304011100 Reaktionen
113041025000 Reaktionen
Katalognummer 11304029
Preis (EUR)
1.938,00
Each
Anzahl Reaktionen:
500 Reaktionen
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
1.938,00
Each
Platinum™ Taq High-Fidelity DNA-Polymerase eignet sich ideal für Amplifikationen von DNA-Fragmenten, wenn hohe Ausbeuten und eine verlässliche Amplifikation erforderlich sind. Die hohe Genauigkeit wird erzielt durch eine Mischung aus Platinum™ Taq DNA-Polymerase und Proofreading-Enzym (3´→5´-Exonukleaseaktivität) der Pyrococcus-Spezies GB-D-Polymerase. Die PCR-Spezifität ist deutlich besser durch Einbindung der Platinum™ automatischen „Hot-Start“-Technologie. Merkmale von Platinum™ Taq High-Fidelity DNA-Polymerase:

• Wiedergabetreue – mehr als sechs Mal genauer als Taq DNA-Polymerase
• Amplikongröße – Amplifikation von Fragmenten bis zu 15 kb (siehe Abbildung)
• Praktisch – Reaktionsansatz bei Raumtemperatur
• Anwendungen – Amplifikation von DNA aus komplex genomischen, viralen und Plasmid-Templates sowie RT-PCR

Definition einer Einheit
Eine Einheit Platinum™ Taq DNA-Polymerase, hohe Wiedergabetreue, ist die Enzymmenge, die benötigt wird, um in 30 Minuten bei 74 °C 10 nmol Desoxyribonukleotid in die DNA zu integrieren.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Wiedergabetreue (vs. Taq)6X
Hot-StartIntegrierter Heißstart
Anzahl Reaktionen500 Reaktionen
ÜberhangGemischt
PolymerasePlatinum Taq DNA-Polymerase High Fidelity
ProdukttypDNA-Polymerase High Fidelity
Menge500 Reaktionen
ReaktionsformatSeparate Komponenten
VersandbedingungTrockeneis
Größe (Endprodukt)20 kb oder weniger
NachweisverfahrenPrimer-Sonde
Zur Verwendung mit (Anwendung)Heißstart-PCR, PCR mit hoher Wiedergabetreue
GC-Rich PCR PerformanceNiedrig
ReaktionsgeschwindigkeitStandard
Unit SizeEach
Inhalt und Lagerung
• 1 x 100 µl Platinum Taq DNA-Polymerase High Fidelity (5 E/µl)
• 1 x 2,25 ml 10X High Fidelity Puffer [600 mM Tris-SO4 (pH 8,9), 180 mM (NH4) 2SO4]
• 1 x 1 ml 50 mM MgSO4

Bei -10 bis -30 °C lagern.

Häufig gestellte Fragen (FAQ)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all Platinum™ Taq DNA-Polymerase High Fidelity FAQs

Zitierungen und Referenzen (4)

Zitierungen und Referenzen
Abstract
Relation between kidney function, proteinuria, and adverse outcomes.
Authors:Hemmelgarn BR, Manns BJ, Lloyd A, James MT, Klarenbach S, Quinn RR, Wiebe N, Tonelli M
Journal:JAMA
PubMed ID:20124537
'The current staging system for chronic kidney disease is based primarily on estimated glomerular filtration rate (eGFR) with lower eGFR associated with higher risk of adverse outcomes. Although proteinuria is also associated with adverse outcomes, it is not used to refine risk estimates of adverse events in this current system.' ... More
Factor B and the mitochondrial ATP synthase complex.
Authors: Belogrudov Grigory I; Hatefi Youssef;
Journal:J Biol Chem
PubMed ID:11744738
Factor B is a subunit of the mammalian ATP synthase complex, whose existence has been controversial. This paper describes the molecular and functional properties of a recombinant human factor B, which when added to bovine submitochondrial particles depleted of their factor B restores the energy coupling activity of the ATP ... More
Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.
Authors:Goshima N, Kawamura Y, Fukumoto A, Miura A, Honma R, Satoh R, Wakamatsu A, Yamamoto J, Kimura K, Nishikawa T, Andoh T, Iida Y, Ishikawa K, Ito E, Kagawa N, Kaminaga C, Kanehori K, Kawakami B, Kenmochi K, Kimura R, Kobayashi M, Kuroita T, Kuwayama H, Maruyama Y, Matsuo K, Minami K, Mitsubori M, Mori M, Morishita R, Murase A, Nishikawa A, Nishikawa S, Okamoto T, Sakagami N, Sakamoto Y, Sasaki Y, Seki T, Sono S, Sugiyama A, Sumiya T, Takayama T, Takayama Y, Takeda H, Togashi T, Yahata K, Yamada H,
Journal:Nat Methods
PubMed ID:19054851
Appropriate resources and expression technology necessary for human proteomics on a whole-proteome scale are being developed. We prepared a foundation for simple and efficient production of human proteins using the versatile Gateway vector system. We generated 33,275 human Gateway entry clones for protein synthesis, developed mRNA expression protocols for them ... More
Mutation screening in 86 known X-linked mental retardation genes by droplet-based multiplex PCR and massive parallel sequencing.
Authors:Hu H, Wrogemann K, Kalscheuer V, Tzschach A, Richard H, Haas SA, Menzel C, Bienek M, Froyen G, Raynaud M, Van Bokhoven H, Chelly J, Ropers H, Chen W
Journal:Hugo J
PubMed ID:21836662
Massive parallel sequencing has revolutionized the search for pathogenic variants in the human genome, but for routine diagnosis, re-sequencing of the complete human genome in a large cohort of patients is still far too expensive. Recently, novel genome partitioning methods have been developed that allow to target re-sequencing to specific ... More