Ham's F-12 Nährstoffmischung

Zitierungen und Referenzen (5)

Gibco™

Ham's F-12 Nährstoffmischung

Name: Ham's F-12 Nährstoffmischung
SKU: 11765047
Price: 30.46 EUR

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11765047	1000 mL
11765054	500 mL

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Katalognummer 11765047

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1000 mL

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Preis (EUR)

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Ham F-12-Nährstoffmischung (F-12) wurde speziell für die Serum-freie Einzelzell-Ausplattierung von CHO-Zellen (Chinese Hamster Ovary) entwickelt. F-12 wurde seitdem für Serum-freies Wachstum von CHO-Kulturen sowie Serum-ergänztes Wachstum anderer Säugetierzellen, einschließlich Chondrozyten und Prostata-Epithelzellen der Ratte, verwendet. Wir führen verschiedene F-12-Modifikationen für eine Vielzahl von Zellkulturanwendungen. Finden Sie die richtige Formulierung mithilfe des Medien-Auswahlwerkzeugs.

Dieses Ham F-12 wird wie folgt modifiziert:

mit

• L-Glutamin

• Phenolrot

Die vollständige Zusammensetzung ist verfügbar.

Verwendung von F-12
Im Vergleich zu anderen basalen Medien enthält F-12 eine breitere Vielzahl von Komponenten, einschließlich Zink, Putrescin, Hypoxanthin und Thymidin. F-12 enthält keine Proteine, Lipide oder Wachstumsfaktoren. Aus diesem Grund ist bei F-12 eine Supplementierung, meist mit 10 % fötalem Rinderserum (FBS), erforderlich. F-12 nutzt ein Puffersystem mit Natriumbikarbonat (1,176 g/l) und erfordert daher eine Umgebung aus 5 bis 10 % CO₂, um den physiologischen pH-Wert aufrechtzuerhalten.

cGMP-konformes Fertigungs- und Qualitätssystem
Um eine kontinuierliche Versorgung zu gewährleisten, produzieren wir F-12 in zwei unterschiedlichen Einrichtungen in Grand Island, NY, USA und in Schottland, Großbritannien. Beide Standorte erfüllen die cGMP-Fertigungsanforderungen und sind zertifiziert nach ISO 13485 und bei der FDA als Hersteller medizinischer Geräte registriert.

Specifications

ZelllinieCHO, COS-7 und Prostataepithelzellen der Ratte

ZelltypPrimäre Ratten-Astrozyten

Konzentration1 X

Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard

ProduktlinieGibco

ProdukttypHam's F-12 Nährstoffmischung

Menge1000 mL

Haltbarkeit12 Monate ab Herstellungsdatum

VersandbedingungRaumtemperatur

KlassifikationOhne Stoffe tierischen Ursprungs

FormFlüssig

SterilitätSteril gefiltert

Sterilization MethodSterile-filtered

Mit AdditivenGlutamin, Phenolrot, Natriumpyruvat

Ohne AdditiveKeine HEPES

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen: 2 bis 8 °C, vor Licht schützen
Versandbedingungen: Haltbarkeit bei
Raumtemperatur: 12 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Ham's F-12 Nährstoffmischung FAQs

Zitierungen und Referenzen (5)

Zitierungen und Referenzen

Abstract

Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.

Authors: Takagi Y; Ninomiya H; Sakamoto A; Miwa S; Masaki T;

Journal:J Biol Chem

PubMed ID:7730310

'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type ... More

Molecular cloning and functional analysis of the promoter of the human squalene synthase gene.

Authors: Guan G; Jiang G; Koch R L; Shechter I;

Journal:J Biol Chem

PubMed ID:7665618

'We have cloned and characterized the 5''-flanking region of the gene encoding human squalene synthase. We report here the promoter activity of successively 5''-truncated sections of a 1 kilobase of this region by fusing it to the coding region of a luciferase reporter gene. DNA segments of 200 base pairs ... More

Functional analysis of the human D2 dopamine receptor missense variants.

Authors: Cravchik A; Sibley D R; Gejman P V;

Journal:J Biol Chem

PubMed ID:8824240

The human dopamine D2 receptor gene (DRD2) has three polymorphic variants that predict the amino acid substitutions Val96 --> Ala, Pro310 --> Ser, and Ser311 --> Cys in the receptor protein. We have investigated the ligand binding and signal transduction properties of these human D2 receptor variants by stably expressing ... More

Requirement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor for selected GH-stimulated function.

Authors: Lobie P E; Allevato G; Nielsen J H; Norstedt G; Billestrup N;

Journal:J Biol Chem

PubMed ID:7665593

We have examined the involvement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor in the cellular response to GH. Stable Chinese hamster ovary (CHO) cell clones expressing a receptor with tyrosine residues at position 333 and 338 of the receptor substituted for phenylalanine (CHO-GHR1-638 Y333F, Y338F) ... More

Mutational analysis of the insulin-like growth factor I prohormone processing site.

Authors: Duguay S J; Lai-Zhang J; Steiner D F;

Journal:J Biol Chem

PubMed ID:7615562

Insulin-like growth factor I (IGF-I) is a mitogenic peptide that is produced in most tissues and cell lines and plays an important role in embryonic development and postnatal growth. IGF-I is initially synthesized as a prohormone precursor that is converted to mature IGF-I by endoproteolytic removal of the carboxyl-terminal E-domain. ... More