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Invitrogen™
SuperScript V VILO Master Mix
Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications.
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| Katalognummer | Anzahl Reaktionen | Enthält |
|---|---|---|
| 11800112 | 500 Reactions | SuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water |
| 11800001 | 50 Reactions | SuperScript V VILO Master Mix, No RT Control, Water |
| 11800002 | 500 Reactions | SuperScript V VILO Master Mix, No RT Control, Water |
| 11800003 | 2000 Reactions | SuperScript V VILO Master Mix, No RT Control, Water |
| 11800111 | 50 Reactions | SuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water |
| 11800113 | 2000 Reactions | SuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water |
| 11800004 | 1250 Reactions | SuperScript V VILO Master Mix only |
Katalognummer 11800112
Preis (EUR)
6.560,00
Each
Anzahl Reaktionen:
500 Reactions
Enthält:
SuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
Preis (EUR)
6.560,00
Each
Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix is also available with ezDNase Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.
Features of SuperScript V VILO Master Mix include:
- Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
- Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
- Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
- Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
- Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility
SuperScript V VILO Master Mix combines SuperScript V RT, RNase inhibitor, buffer, dNTPs, and an optimized blend of oligo(dT) and random primers in a single formulation, simplifying reaction setup and helping reduce variability. Its combination of fast cDNA synthesis, robust performance, and flexible room-temperature handling makes it well suited for routine, high-throughput, and automated RT-qPCR workflows.
SuperScript V VILO Master Mix with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA, further simplifying the two-step RT-qPCR workflow.
A DTT-free, animal origin-free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
EndprodukttypFirst-Strand cDNA
FormatMaster Mix
EnthältSuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
Inhibitor Tolerance (Simple)High
Anzahl Reaktionen500 Reactions
Optimale Reaktionstemperatur50°C
ProdukttypReverse Transcription Reagent
ReagenztypReverse Transcription
Reverse TranskriptaseSuperScript V
Ribonuklease-H-AktivitätReduced
Empfindlichkeit0.1 pg total RNA
VersandbedingungDry Ice, Wet Ice, Ambient
Größe (Endprodukt)Up to 10 kb
AusgangsmaterialRNA
VerfahrenReverse Transcription
ThermostabilitätUp to 65°
Konzentration5X
NachweisverfahrenqPCR, NGS, PCR
Zur Verwendung mit (Anwendung)Gene Expression, Real Time PCR (qPCR), RT-PCR, cDNA Synthesis
FormLiquid
GC-Rich PCR PerformanceHigh
Marker oder FarbstoffNot Applicable
PCR-Methode2-step RT-qPCR, RT-PCR
Reaktionsgeschwindigkeit10 min.
Unit SizeEach
Inhalt und Lagerung
• SuperScript V VILO Master Mix (2 x 1 mL)
• SuperScript V VILO No RT Control (1 mL)
• ezDNase Master Mix (500 μL)
• Nuclease-free water (1.25 mL)
Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 5 months
• Room temperature for up to 1 month
• SuperScript V VILO No RT Control (1 mL)
• ezDNase Master Mix (500 μL)
• Nuclease-free water (1.25 mL)
Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 5 months
• Room temperature for up to 1 month