What alternative do you offer for Actinomycin D (Cat. No. 11805017)?
The alternative for Actinomycin D, 5 mg (Cat. No. 11805017) is Actinomycin D, 10 mg (Cat. No. A7592).
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What is the stability and storage recommendations for Actinomycin D (Cat. No. 11805017) once it is reconstituted?
We recommend storing Actinomycin D (Cat. No. 11805017) before reconstitution at 2-8 degrees C. Once the product is reconstituted, we recommend storing it in a freezer (-5 to -20 degrees C), protected from light.
The shelf life of the powder is 1 year from the date of shipment. After reconstitution, the product can be stored frozen for up to 4 weeks.
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How should I reconstitute Actinomycin D?
We recommend reconstituting Actinomycin D in either DMF or DMSO; it should be soluble to at least 1 mM in either solvent.
How can I decontaminate my cultures?
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
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What antibiotics do you offer to help control or eliminate cell culture contamination?
Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
