Oligofectamine™ Transfektionsreagenz

Zitierungen und Referenzen (48)

Invitrogen™

Oligofectamine™ Transfektionsreagenz

Katalognummer	Menge
12252011	1 ml

Katalognummer 12252011

Preis (EUR)

1.002,00

Zum Warenkorb hinzufügen

Menge:

Preis (EUR)

1.002,00

Zum Warenkorb hinzufügen

Oligofectamine™ Transfektionsreagenz ist eine geschützte Formulierung für die Transfektion von Oligonukleotiden und Short interfering RNA (siRNA) in eukaryotischen Zellen. Oligofectamine™ Transfektionsreagenz bildet stabile Komplexe mit Oligos, was die effiziente Transfektion in eukaryotischen Zellen auf äußerst spezifische, aber ungiftige Weise ermöglicht. Oligofectamine™ Reagenz ist für nukleare und zytoplasmatische Ziele geeignet und transfiziert eine Vielzahl von Zelllinien, einschließlich CHO, HEK-293, NIH 3T3 und HeLa.

Verwendung von Oligofectamine™ Transfektionsreagenz
Oligofectamine™ Reagenz ist aufgrund des simplen und schnellen Protokolls problemlos anzuwenden. Verdünnen Sie Oligofectamine™ Reagenz einfach, indem Sie es mit Oligonukleotid mischen, und fügen Sie es Ihren Zellen hinzu. Oligofectamine™ Reagenz erfordert nanomolare Mengen an Antisense-Oligonukleotiden, was zu einer bis zu 1.000-fachen Verringerung der benötigten Menge an wertvollen Oligonukleotiden führt. Dies macht es ideal für Anwendungen mit hohem Durchsatz. Außerdem ist Oligofectamine™ Reagenz nachweislich auch für siRNA-Transfektionen geeignet. Wir empfehlen dieses Reagenz bei der Durchführung von RNAi Knockdown-Experimenten in HeLa-Zellen. Weitere Informationen finden Sie bei RNAi Central.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Zur Verwendung mit (Anwendung)Transfektion

Hochdurchsatz-KompatibilitätGeeignet für hohe Durchsätze

ProduktlinieOligofectamine

ProdukttypTransfektionsreagenz

Menge1 ml

Serum-kompatibelNein

VersandbedingungNasseis

ZelltypEtablierte Zelllinien, Primärzellen, schwer transfizierbare Zellen

Format6-Well-Platte, 12-Well-Platte, 24-Well-Platte, 48-Well-Platte, 96-Well-Platte, Kolben

ProbentypSynthetische siRNA

Transfection TechniqueLipid-basierte Transfektion

Unit SizeEach

Inhalt und Lagerung

Enthält ein Fläschchen (1 ml) des Reagenz Oligofectamine™. Bei 4 °C lagern. Nicht einfrieren.

Häufig gestellte Fragen (FAQ)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

View all Oligofectamine™ Transfektionsreagenz FAQs

Zitierungen und Referenzen (48)

Zitierungen und Referenzen

Abstract

A high-throughput, cell-based screening method for siRNA and small molecule inhibitors of mTORC1 signaling using the In Cell Western technique.

Authors:Hoffman GR, Moerke NJ, Hsia M, Shamu CE, Blenis J,

Journal:Assay Drug Dev Technol

PubMed ID:20085456

'The mTORC1 pathway is a central regulator of cell growth, and defective mTORC1 regulation plays a causative role in a variety of human diseases, including cancer, tumor syndromes such as the tuberous sclerosis complex (TSC) and lymphangioleiomyomatosis (LAM), and metabolic diseases such as diabetes and obesity. Given the importance of ... More

Processing of Pro-atrial Natriuretic Peptide by Corin in Cardiac Myocytes.

Authors: Wu Faye; Yan Wei; Pan Junliang; Morser John; Wu Qingyu;

Journal:J Biol Chem

PubMed ID:11884416

'Corin is a type II transmembrane serine protease abundantly expressed in the heart. In a previous study using transfected 293 cells, we showed that corin converted pro-atrial natriuretic peptide (pro-ANP) to atrial natriuretic peptide (ANP), suggesting that corin is likely the pro-ANP convertase. Because other serine proteases such as thrombin ... More

Systems survey of endocytosis by multiparametric image analysis.

Authors:Collinet C, Stöter M, Bradshaw CR, Samusik N, Rink JC, Kenski D, Habermann B, Buchholz F, Henschel R, Mueller MS, Nagel WE, Fava E, Kalaidzidis Y, Zerial M,

Journal:Nature

PubMed ID:20190736

'Endocytosis is a complex process fulfilling many cellular and developmental functions. Understanding how it is regulated and integrated with other cellular processes requires a comprehensive analysis of its molecular constituents and general design principles. Here, we developed a new strategy to phenotypically profile the human genome with respect to transferrin ... More

N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic.

Authors:Glozman R, Okiyoneda T, Mulvihill CM, Rini JM, Barriere H, Lukacs GL,

Journal:J Cell Biol

PubMed ID:19307599

'N-glycosylation, a common cotranslational modification, is thought to be critical for plasma membrane expression of glycoproteins by enhancing protein folding, trafficking, and stability through targeting them to the ER folding cycles via lectin-like chaperones. In this study, we show that N-glycans, specifically core glycans, enhance the productive folding and conformational ... More

Disinhibition of neurotrophin-induced dorsal root ganglion cell neurite outgrowth on CNS myelin by siRNA-mediated knockdown of NgR, p75(NTR) and Rho-A.

Authors:Ahmed Z, Dent RG, Suggate EL, Barrett LB, Seabright RJ, Berry M, Logan A,

Journal:Mol Cell Neurosci

PubMed ID:15737741

'The presence of multiple axon growth inhibitors may partly explain why central nervous system axons are generally incapable of regenerating after injury. Using RNA interference (RNAi) in dorsal root ganglia neurons (DRGN), we demonstrate siRNA-mediated silencing of components of the inhibitory signalling cascade, including p75(NTR), NgR and Rho-A mRNA, of ... More

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