Nunc™ Polycarbonat-Zellkultureinsätze in Multi-Well-Platten

Thermo Scientific™

Nunc™ Polycarbonat-Zellkultureinsätze in Multi-Well-Platten

Name: Nunc™ Polycarbonat-Zellkultureinsätze in Multi-Well-Platten
SKU: 140644
Price: 239.00 EUR

Kultivieren Sie die meisten Zelltypen ohne Matrixbeschichtung mit den Thermo Scientific™ Nunc™ Polycarbonat-Membraneinsätzen in Multischalen.

Ansicht ändern

Katalognummer	Porengröße	Schalentyp	Kulturfläche
140644	8 μm	6-Well-Platte	3,14cm²
140620	0,4 μm	24-Well-Platte	0,47 cm²
140627	3 μm	24-Well-Platte	0,47 cm²
140629	8 μm	24-Well-Platte	0,47 cm²
140640	0,4 μm	6-Well-Platte	3,14cm²
140642	3 μm	6-Well-Platte	3,14cm²
140652	0,4 μm	12-Well-Platte	1,13 cm²
140654	3 μm	12-Well-Platte	1,13 cm²
140656	8 μm	12-Well-Platte	1,13 cm²
140660	0,4 μm	6-Well-Platte	4,1cm²
140663	3 μm	6-Well-Platte	4,1cm²
140668	8 μm	6-Well-Platte	4,1cm²

12 Optionen

Katalognummer 140644

Preis (EUR)

239,00

Case of 24

Zum Warenkorb hinzufügen

Porengröße:

8 μm

Schalentyp:

6-Well-Platte

Kulturfläche:

3,14cm²

Preis (EUR)

239,00

Case of 24

Zum Warenkorb hinzufügen

Kultivieren Sie die meisten Zelltypen ohne Matrixbeschichtung mit den Thermo Scientific™ Nunc™ Polycarbonat-Membraneinsätzen in Multischalen.

Merkmale:

Gewebekulturbehandelte poröse Membran für optimierte Zelladhäsion
Drei Porengrößen: 0,4, 3 und 8 Mikrometer
Vorgepackt in sterilen Nunclon™ Delta-behandelten Multiwellplatten
Nicht toxisch und pyrogenfrei

Anwendungen:

Tumormigration und Invasionsassays
Epitheliale (z. B. CaCO-2) und endotheliale (z. B. mikrovaskuläre Zellen im Gehirn) blockieren Assays
Dermale/epidermale und epitheliale Gewebemodelle (z. B. künstliche Hautmodelle)
Chemotaxisstudien
Co-Kultur

Garantie: 90 Tage

Aufhebung der Nutzungsbeschränkung auf interne Forschungszwecke: Jegliche dem Käufer auferlegte Beschränkung, das/die Produkt(e) nur zu internen Forschungszwecken zu nutzen, gilt nicht für das/die Produkt(e) auf Webseiten, die diese Erklärung enthalten.

Allgemeine Referenzen:

Mardi A Crane-Godreau et.al (2005). CCL20/macrophage Inflammatory Protein 3alpha and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment With Lipopolysaccharide or Pam3Cys. PMID: 15618187
Rettinger CL et. al (2018). Quantitative Assessment of Retina Explant Viability in a Porcine Ex Vivo Neuroretina Model. PMID: 29924674
Mardi A Crane-Godreau et.al (2004). Effect of Escherichia coli and Lactobacillus rhamnosus on macrophage inflammatory protein 3 alpha, tumor necrosis factor alpha, and transforming growth factor beta release by polarized rat uterine epithelial cells in culture. PMID: 15039305
Soboll G et. al (2006). Effect of oestradiol on PAMP-mediated CCL20/MIP-3 alpha production by mouse uterine epithelial cells in culture. PMID: 16771853
Mardi A Crane-Godreau et. al (2009). Exposure to Cigarette Smoke Disrupts CCL20-Mediated Antimicrobial Activity in Respiratory Epithelial Cells. PMID: 19966927
Wildemann B et. al (2005). Differences in the fusion and resorption activity of human osteoclasts after stimulation with different growth factors released from a polylactide carrier. PMID: 15477999
Sugita Y et. al (1997). Pigment epithelium-derived factor (PEDF) has direct on the metabolism and proliferation of microglia and indirect effects on astrocytes. PMID: 9335258
van Schilfgaarde M et. al (1999). Haemophilus influenzae localized in epithelial cell layers is shielded from antibiotics and antibody-mediated bactericidal activity. PMID: 10222210
Chung HC et. al (2014). Inhibition of porcine endogenous retrovirus in PK15 cell line by efficient multitargeting RNA interference. PMID: 24138389
Ode A et. al (2010). Toward biomimetic materials in bone regeneration: functional behavior of mesenchymal stem cells on a broad spectrum of extracellular matrix components. PMID: 20878902

Specifications

FarbeKlar

BeschreibungPolycarbonat-Zellkultureinsätze mit einer Porengröße von 8 Mikrometer in 6-Well-Mikrotiterplatte

Schalentyp6-Well-Platte

Anzahl pro Karton24

Anzahl pro Packung6

OberflächenbehandlungCell ID

Kulturfläche3,14cm²

Zur Verwendung mitMultischale 6

Einsätze pro Platte6

MaterialPolycarbonat (Membran)

Anzahl Wells6

Porendichte< 0,85 x 10^5

Porengröße8 μm

SterilitätSteril

TypKultureinsatz

Arbeitsvolumen (metrisch)1.5mL

Unit SizeCase of 24

Häufig gestellte Fragen (FAQ)

What is the difference between the 24-well plate format for the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620) and the 24-well plate format for the Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002)?

The difference between the two 24-well plate formats is as follows:

Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620): There are only 12 inserts in the 24-well multi-well plate, leaving every second column free without an insert. This is a preferred configuration for some co-culture experiments, as it makes it easy to access the culture grown in the multi-well plate for media changes, etc., by placing the insert in the empty well next to it. The inserts are placed directly into the wells here, leaving a 0.9 mm gap between the bottom of the well and the insert membrane.
Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002): There are 24 inserts in the 24-well carrier plate. The carrier plate with the hanging slots allows for adjustment of the insert height. The three hanging positions in the carrier plate allow for distance of approximately 0.9 mm, 3.3 mm, or 6.3 mm between the well-bottom and insert growth surface for both 12- and 24-well plate formats. This configuration is preferred when customer needs more versatility, for example to facilitate air-liquid interface culture, and other co-culture applications.

Note: details on plate configuration can also be found within the following product brochure (pg 3).

With the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates, what is the distance between the cell culture membrane and the bottom of the multi-well dish?

The membrane is approximately 1.0 mm above the bottom of the dish for the Nunc Polycarbonate Cell Culture Inserts.

Can I observe cells on the cell culture membrane when the Nunc Polycarbonate Cell Culture Insert is inside of a multi-well plate?

Due to the large pore size of the Nunc polycarbonate inserts, spots or holes are observed and they represent the membrane. In this plane of focus, the most visible cells are the ones located on the membrane, whereas the slightly blurred backdrop are the cells on the dish that are in a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of the membrane. However, these cells are visible in culture.

What is the recommended Multidish chamber media volume per carrier plate insert and height position?

Low position: 2 mL (12-well), 1 mL (24-well), Medium position: 3 mL (12-well), 1.5 mL (24-well), High position: 4 mL (12-well), 2 mL (24-well). Some optimization may be necessary for development purposes.

Why are only 12 inserts provided with standard membrane insert products in the 24-well format?

This has emerged as the industry standard. In many cases having empty wells facilitates media changes and other experimental steps.

View all Nunc™ Polycarbonat-Zellkultureinsätze in Multi-Well-Platten FAQs