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Antibiotika-Antimykotikum (100x)
Zitierungen und Referenzen (13)
Gibco™

Antibiotika-Antimykotikum (100x)

Gibco™ Antibiotikum-Antimykotikum dient zur Verhinderung von Kontamination durch Bakterien und Pilze. Diese Lösung enthält 10.000 Einheiten/ml Penicillin, 10.000 µg/ml StreptomycinWeitere Informationen
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KatalognummerMenge
15240062100 mL
1524009620 mL
Katalognummer 15240062
Preis (EUR)
92,65
Precio exclusivo en nuestra web
102,00
Ersparnis 9,35 (9%)
Each
Menge:
100 mL
Preis (EUR)
92,65
Precio exclusivo en nuestra web
102,00
Ersparnis 9,35 (9%)
Each
Gibco™ Antibiotikum-Antimykotikum dient zur Verhinderung von Kontamination durch Bakterien und Pilze. Diese Lösung enthält 10.000 Einheiten/ml Penicillin, 10.000 µg/ml Streptomycin und 25 µg/ml Gibco Amphotericin B. Die Antibiotika Penicillin und Streptomycin verhindern durch ihre effektive kombinierte Wirksamkeit gegen grampositive und gramnegative Bakterien die bakterielle Kontamination von Zellkulturen. Amphotericin B verhindert Pilzkontaminationen in Zellkulturen aufgrund seiner Hemmung von multizellulären Pilzen und Hefen.

Penicillin, das ursprünglich aus dem Pilz Penicillium gewonnen wurde, wirkt direkt, indem es den Neuaufbau der bakteriellen Zellwand stört, sowie indirekt, indem es die Freisetzung von Enzymen auslöst, die die Zellwand weiter verändern. Streptomycin wurde ursprünglich aus Streptomyces griseus extrahiert. Es wirkt, indem es sich an die 30S-Untereinheit des bakteriellen Ribosoms anlagert, was zu einer Hemmung der Protein-Synthese und anschließendem Absterben anfälliger Bakterien führt. Amphotericin B ist ein Fungizid, das das Wachstum von Pilzen und Hefen durch die Erhöhung der Durchlässigkeit der fungalen Plasmamembran verhindert.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications
Konzentration100X
KulturtypSäugetierzellkultur
Menge100 mL
Haltbarkeit12 Monate
VersandbedingungTrockeneis
FormFlüssig
ProdukttypAntibiotikum-Antimykotikum
SterilitätSteril gefiltert
Sterilization MethodSterile-filtered
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen: -5 bis -20 °C
Versandbedingungen: Trockeneis
Haltbarkeit: 12 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

Can I use pen/strep or other antibiotics for episomal reprogramming of cells?

The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?

Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the solvent used in Antibiotic-Antimycotic (100X)?

The components are in a 0.85% saline solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the components of Antibiotic-Antimycotic (100X)?

This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition for Antibiotic-Antimycotic (100X)?

The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Antibiotika-Antimykotikum (100x) FAQs

Zitierungen und Referenzen (13)

Zitierungen und Referenzen
Abstract
Common variation at 6q16 within HACE1 and LIN28B influences susceptibility to neuroblastoma.
Authors:Diskin SJ, Capasso M, Schnepp RW, Cole KA, Attiyeh EF, Hou C, Diamond M, Carpenter EL, Winter C, Lee H, Jagannathan J, Latorre V, Iolascon A, Hakonarson H, Devoto M, Maris JM,
Journal:Nat Genet
PubMed ID:22941191
Neuroblastoma is a cancer of the sympathetic nervous system that accounts for approximately 10% of all pediatric oncology deaths. Here, we report a genome-wide association study of 2,817 neuroblastoma cases and 7,473 controls. We identified two new associations at 6q16, the first within HACE1 (rs4336470; combined P=2.7×10(-11); odds ratio 1.26, ... More
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Single-Cell Transcriptome Atlas of Murine Endothelial Cells.
Authors:Kalucka J, de Rooij LPMH, Goveia J, Rohlenova K, Dumas SJ, Meta E, Conchinha NV, Taverna F, Teuwen LA, Veys K, García-Caballero M, Khan S, Geldhof V, Sokol L, Chen R, Treps L, Borri M, de Zeeuw P, Dubois C, Karakach TK, Falkenberg KD, Parys M, Yin X, Vinckier S, Du Y, Fenton RA, Schoonjans L, Dewerchin M, Eelen G, Thienpont B, Lin L, Bolund L, Li X, Luo Y, Carmeliet P
Journal:Cell
PubMed ID:32059779
'The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7' ... More
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