Zitierungen und Referenzen (10)

Gibco™

Fötales Kälberserum, zertifiziert, USA

Name: Fötales Kälberserum, zertifiziert, USA
SKU: 16000044

Gibco fötale Kälberseren bieten einen ausgezeichneten Wert für die allgemeine Zellkultur, die Spezialforschung und spezifische Assays. Sie erhalten das VertrauenWeitere Informationen

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Katalognummer	Menge	Format
16000044	500 mL	Flasche
A3160401	50 mL	One Shot
A3160402	10 x 50 mL	One Shot

3 Optionen

Our improved Premium Plus Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.

Katalognummer 16000044

Gibco fötale Kälberseren bieten einen ausgezeichneten Wert für die allgemeine Zellkultur, die Spezialforschung und spezifische Assays. Sie erhalten das Vertrauen von Forschern mit gleichbleibender Qualität und prämierter Unterstützung, die Ihren Forschungsanforderungen und Ihrem Budget gerecht wird

Gibco Serum: Bewährte Qualität für konsistente Leistung

Serum-Kategorie: Premium (Performance Plus)
• Zur Verwendung für eine Vielzahl von Zellarten, insbesondere für empfindliche Zelllinien
• Unser am besten charakterisiertes Serum mit dem niedrigsten Endotoxin-Gehalt und Hämoglobin-Spiegel
• Endotoxin-Gehalt: ≤ 5 EU/ml
• Hämoglobin-Spiegel: ≤ 15 mg/dl
• Herkunft: Vereinigte Staaten

Gibco Serum hält, was es verspricht
• ISO 13485-zertifiziert, wird in Einrichtungen mit FDA-Zulassung verarbeitet
• Für die In-vitro-Diagnostik gekennzeichnet (konform mit höchsten USP-Sterilitätsstandards)
• Dreifach gefiltert auf 0,1 µm
• Gibco Flaschen sind unter Abzugshauben leichter zu verwenden, reduzieren das Kontaminationsrisiko und unterstützen Sie bei der konsistenteren Züchtung von Zellkulturen.
• Der Abziehaufkleber auf dem Etikett ermöglicht eine praktische Referenz auf die in der Labordokumentation Chargennummer und Verfallsdatum im Laborheft.

Für die Verwendung in der Forschung oder die Weiterverarbeitung. Nicht zur Diagnosestellung oder direkten Anwendung bei Menschen oder Tieren geeignet.

Specifications

Endotoxin-Konzentration≤5 EU/mL

Hämoglobin-Konzentration≤15 mg/dl

Reinheits- oder QualitätsgradZertifiziert

Versandbedingunggefroren

SpeziesRind/Bovine

AgeFötal

HerkunftslandVereinigte Staaten

FormFlüssig

FormatFlasche

ProdukttypFötales Kälberserum

Menge500 mL

Serum TreatmentStandard (sterilfiltriert)

SterilitätSteril

Sterilization MethodDreifach gefiltert, 0.1 μm

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen: ≤ -10 ° C
Versandbedingungen: Gefroren

Häufig gestellte Fragen (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fötales Kälberserum, zertifiziert, USA FAQs

Zitierungen und Referenzen (10)

Zitierungen und Referenzen

Abstract

Fibroblast growth factor receptor-1 signaling induces osteopontin expression and vascular smooth muscle cell-dependent adventitial fibroblast migration in vitro.

Authors: Li Guohong; Oparil Suzanne; Kelpke Stacey S; Chen Yiu-Fai; Thompson John A;

Journal:Circulation

PubMed ID:12176960

'BACKGROUND: Increased expression of osteopontin (OPN), fibroblast growth factors (FGFs), and their type-1 receptor (FGFR-1) is associated with neointima formation and atherosclerosis. This study tested the hypothesis that ligand activation of FGFR-1 stimulates OPN expression in rat aortic smooth muscle cells (RASMCs), explored the signaling pathway involved, and assessed the ... More

Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells.

Authors:Li T, Dai W, Lu L.

Journal:J Biol Chem

PubMed ID:12082101

'The exposure of mammalian cells to UV irradiation induces the expression of immediate early genes such as c-jun and c-fos and activates the transcription factors AP-1 and NF-kappaB. JunD is one of the three members of the Jun family and shares some functional characteristics with c-Jun. In the present study, ... More

Dystrophin deficiency markedly increases enterovirus-induced cardiomyopathy: a genetic predisposition to viral heart disease.

Authors: Xiong Dingding; Lee Gil-Hwan; Badorff Cornel; Dorner Andrea; Lee Sang; Wolf Paul; Knowlton Kirk U;

Journal:Nat Med

PubMed ID:12118246

Both enteroviral infection of the heart and mutations in the dystrophin gene can cause cardiomyopathy. Little is known, however, about the interaction between genetic and acquired forms of cardiomyopathy. We previously demonstrated that the enteroviral protease 2A cleaves dystrophin; therefore, we hypothesized that dystrophin deficiency would predispose to enterovirus-induced cardiomyopathy. ... More

Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival.

Authors: Sotiriou Sotiria; Gispert Suzana; Cheng Jun; Wang Yaohui; Chen Amy; Hoogstraten-Miller Shelley; Miller Georgina F; Kwon Oran; Levine Mark; Guttentag Susan H; Nussbaum Robert L;

Journal:Nat Med

PubMed ID:11984580

The only proven requirement for ascorbic acid (vitamin C) is in preventing scurvy, presumably because it is a cofactor for hydroxylases required for post-translational modifications that stabilize collagen. We have created mice deficient in the mouse ortholog (solute carrier family 23 member 1 or Slc23a1) of a rat ascorbic-acid transporter, ... More

Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device.

Authors: Yang Mengsu; Li Cheuk-Wing; Yang Jun;

Journal:Anal Chem

PubMed ID:12199565

We have developed a microfluidic device for on-chip monitoring of cellular reactions. The device consists of two primary analytical functions: control of cell transport and immobilization, and dilution of an analyte solution to generate a concentration gradient. In this device, a dam structure in parallel to the fluid flow was ... More

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