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FKS für embryonale Stammzellen, qualifiziert, One Shot™-Format, US-Ursprung
Gibco™

FKS für embryonale Stammzellen, qualifiziert, One Shot™-Format, US-Ursprung

Serum-Kategorie: SpezialFötales Kälberserum, ES-Zellenqualifiziert wurde speziell bezüglich einer homogenen undifferenzierten zellulären Morphologie von embryonalen Stammzellen getestet. Es ist die GibcoWeitere Informationen
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KatalognummerMengeFormat
16141079500 mLFlasche
1614100250 mLFlasche
Katalognummer 16141079
Preis (EUR)
1.050,00
Each
Menge:
500 mL
Format:
Flasche
Preis (EUR)
1.050,00
Each
Serum-Kategorie: Spezial
Fötales Kälberserum, ES-Zellenqualifiziert wurde speziell bezüglich einer homogenen undifferenzierten zellulären Morphologie von embryonalen Stammzellen getestet. Es ist die Gibco FKS-Qualität und -Leistung, der Sie vertrauen, angeboten in einem Einweg-FKS-Aliquot, das mehr Komfort bietet, Zeit spart und einfach zu verwenden ist.
Für In-vitro-Diagnostik.
Specifications
Reinheits- oder QualitätsgradES-Zellenqualifiziert
Versandbedingunggefroren
SpeziesRind/Bovine
AgeFötal
HerkunftslandVereinigte Staaten
FormFlüssig
FormatFlasche
ProdukttypFötales Kälberserum
Menge500 mL
Serum TreatmentStandard (sterilfiltriert)
SterilitätSteril
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen: ≤ -10 ° C
Versandbedingungen: Gefroren

Häufig gestellte Fragen (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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