Invitrogen™

SuperScript™ II Reverse Transkriptase

Name: SuperScript™ II Reverse Transkriptase
SKU: 18064071
Price: 1.102.65 EUR

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Katalognummer	Anzahl Reaktionen
18064071	200 Reaktionen
18064022	10 Reaktionen
18064014	50 Reaktionen

3 Optionen

Katalognummer 18064071

Preis (EUR)

1.102,65

キャンペーン価格

1.598,00

Ersparnis 495,35 (31%)

Each

Anzahl Reaktionen:

200 Reaktionen

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

1.102,65

キャンペーン価格

1.598,00

Ersparnis 495,35 (31%)

Each

Invitrogen SuperScript II reverse Transkriptase ist eine gentechnisch veränderte reverse MMLV-Transkriptase (RT) mit verringerter RNase H-Aktivität und erhöhter thermischer Stabilität im Vergleich zu Wildtyp-MMLV-RT. Mutationen in der RNase H-Domäne des Enzyms verhindern den Abbau der RNA während der Erststrang-cDNA-Synthese, was zu höheren Ausbeuten von Volllängen-cDNA führt.

Die SuperScript RTs sind die zuverlässigsten und am weitesten verbreiteten RTs mit bis heute über 50.000 Zitaten, Rezensionen und Publikationen.

Hinweis: Das neueste Mitglied der SuperScript RT-Familie, SuperScript IV reverse Transkriptase, bietet verbesserte Thermostabilität, Prozessivität, Ausbeute und Leistung bei beliebigen RNA-Proben, einschließlich solcher mit suboptimalem Reinheitsgrad oder suboptimaler Integrität.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

EndprodukttypcDNA (Erststrang)

FormatEigenständiges Enzym

Anzahl Reaktionen200 Reaktionen

Optimale Reaktionstemperatur42 °C

Menge4 x 10,000 units

ReaktionsformatSeparate Komponenten

ReagenztypReverse Transkription

Reverse TranskriptaseSuperScript II

Ribonuklease-H-AktivitätKeine

VersandbedingungNasseis

Größe (Endprodukt)Bis 12,3 kb

AusgangsmaterialRNA

VerfahrenReverse Transkription

Konzentration200 U/μl

GC-Rich PCR PerformanceHoch

Reaktionsgeschwindigkeit50 min

Unit SizeEach

Inhalt und Lagerung

• SuperScript II Reverse Transkriptase, 4x 10.000 Einheiten (200 E/μl)
• 5X-Erststrangpuffer
• DTT (100 mM)

Lagerung bei –20 °C

Häufig gestellte Fragen (FAQ)

How can I remove genomic DNA contamination from my sample prior to performing RT-PCR?

We recommend using ezDNase (Cat. No. 11766051). ezDNase Enzyme's high specificity for double-stranded DNA enables efficient and fast genomic DNA removal without reduction in the quality or quantity of RNA. ezDNase Enzyme is heat-labile and so can be easily deactivated by heat treatment at moderate temperature (55 degrees C). These features make ezDNase Enzyme an excellent choice for genomic DNA removal prior to reverse transcription reactions.

How much RNA should be employed for first-strand cDNA synthesis?

The amount of RNA template for a cDNA synthesis is highly flexible and depends upon the amount of sample available and an individual's need. In general, 1 µg total RNA is used in a typical 20-µL RT reaction.

Find additional tips, troubleshooting help, and resources within ourReverse Transcription and RACE Support Center.

Should I treat the cDNA with RNase H prior to downstream processing?

RNase H treatment is not always necessary. Many PCR reactions work without it. However, for cDNA synthesized with RNase H-deficient reverse transcriptases (like SuperScript II, III, and IV), RNA/cDNA hybrids—especially GC-rich ones—may not denature well, reducing PCR sensitivity. RNase H treatment can help in such cases. Additionally, RNase H treatment is beneficial for cloning larger fragments.

What percentage of RNA is converted to cDNA when performing reverse transcription?

This depends highly on the quality of the sample. mRNA itself makes up 1-5% of total RNA. Depending on the primer and enzyme used, reverse transcription can covert >70% of that into cDNA.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

I'm setting up my RT reaction and am trying to decide whether I should use random primers, oligo(dT) primer, gene-specific primer, or oligo(dT)/random mix primers. What would you suggest?

Random primers are the best choice for degraded RNA, RNA with heavy secondary structure, non-polyadenylated RNA, or prokaryotic RNA. It is recommended only for two-step RT-PCR, and typically gives the highest yields, although the cDNA may not necessarily be full length. Oligo(dT) primers are good to use when trying to recover full-length cDNA from 2-step RT-PCR. The reaction is influenced by secondary structure and RNA quality. Gene specific primers should be used for very specific, mainly one-step RT-PCR reactions.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

View all SuperScript™ II Reverse Transkriptase FAQs