Thermo Scientific™

Detoxi-Gel™ Endotoxin Removing Columns, 1 mL

Katalognummer	Menge
20344	5 Säulen

Katalognummer 20344

Preis (EUR)

232,00

Menge:

5 Säulen

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

232,00

Das Thermo Scientific Desoxi-Gel Endotoxin-Abbaugel verwendet Polymixin B-Ligand, das auf gekörntem Affinitätsharz immobilisiert ist, um Endotoxine aus Antikörper- oder Proteinproben zu binden und zu extrahieren.

Desoxi-Gel Endotoxin-Abbaugel und Endotoxine (Lipopolysaccharide oder LPS) schnell aus Proben entfernen. Schon niedrige Konzentrationen von Endotoxinen können eine toxische Wirkung auf Zellen und Organismen haben und müssen entfernt werden, bevor Zellen im Rahmen unterschiedlicher Behandlungsexperimente biologischen Proben ausgesetzt werden.

Merkmale von Detoxi-Gel Endotoxin-Abbaugel:

• Harz verwendet immobilisiertes Polymixin B, um Pyrogene durch Bindung ihrer Lipid-A-Domänen zu entfernen
• Reduziert Endotoxinkonzentrationen in Proteinlösungen, Zellkulturmedien, Lösungen mit pharmakologischen Komponenten und wässrigen Puffern
• 1 ml Gel entfernt >9995 EU aus einer 10.000 EU-Challenge von LPS (mehr als 99 % Effizienz)
• 1 ml Gel bindet 4000 bis 6000 E.U. des Lipopolysaccharids aus E. coli-Stamm 055:BS
• Proteinerholung abhängig von Proteintyp und -konzentration; einige empirische Tests erforderlich
• Stabil und wiederverwendbare; Harz kann durch Abisolieren der Endotoxine mit einer 1 % Desoxycholat-Lösung in Pyrogen-armem Wasser regeneriert werden; kein Verlust der Bindungskapazität auch nach 10 Regenerationen

Ähnliche Produkte
Desoxi-Gel™ Endotoxin-Abbaugel

For Research Use Only. Not for use in diagnostic procedures.

Specifications

SäulentypAffinität, Agaroseharz

BeschreibungDetoxi-Gel Endotoxin Removing Columns, 1 ml

FormatZentrifugationssäule

Menge5 Säulen

Stationäre PhasePolymixin B

ZielEndotoxinentfernung

Kapazität (metrisch)1 ml

FormFlüssig

ProduktlinieDetoxi-Gel

TypSäule

Unit SizeEach

Inhalt und Lagerung

Bei 4 °C lagern.

Häufig gestellte Fragen (FAQ)

Why must I regenerate the Detoxi-Gel Endotoxin Removing Gel column before and after each use?

It is possible that the column may have been contaminated with endotoxin during storage and handling. Equilibrating the column with 1% sodium deoxycholate followed by pyrogen-free washing ensures that the column is free of endotoxin before the sample is added. The column must be regenerated following endotoxin removal from a sample to make sure the endotoxin that has bound to the column from the sample is washed off.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the void volume of the Detoxi-Gel Endotoxin Removing Gel column?

The Thermo Scientific 1 mL Detoxi-Gel Column has a void volume of 0.3-0.5 mL. This is the volume of solution that must be collected from the column before the sample will begin to come off the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the protocol for using Detoxi-Gel Endotoxin Removing Gel?

1. Equilibrate the column with 5 column volumes of 1% sodium deoxycholate in pyrogen-free water, followed by 3 column volumes of pyrogen-free water or buffer of choice.
2. Apply sample, being sure not to exceed the binding capacity of the gel.
3. Collect sample by gravity flow by applying additional pyrogen-free water or buffer.
4. Regenerate the column with 5 column volumes of 1% sodium deoxycholate in pyrogen-free water, followed by 3 column volumes of pyrogen-free water.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the binding capacity of Detoxi-Gel Endotoxin Removing Gel?

Detoxi-Gel Support has the capacity to bind up to 2µg LPS/mL of gel. Because one endotoxin unit (EU) is approximately 0.1 ng/mL, the gel has the potential for binding up to 20,000 EU/mL of gel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is Detoxi-Gel Endotoxin Removing Gel, and how does it work?

Detoxi-Gel Support consists of 6% cross-linked beaded agarose that has Polymixin B immobilized onto it. Polymixin B is an antibiotic that contains a cationic cyclopeptide with a fatty acid chain that can neutralize the biological activity of endotoxins by binding to the lipid A portion of the bacterial lipopolysacharide (LPS). After the endotoxin from a sample binds to the immobilized Polymixin B, the sample can be collected and the endotoxin can be eluted using 1% sodium deoxycholate.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Detoxi-Gel™ Endotoxin Removing Columns, 1 mL FAQs