Zitierungen und Referenzen (5)

Gibco™

Ham's F-12K (Kaighn's) Medium

Name: Ham's F-12K (Kaighn's) Medium
SKU: 21127022
Price: 54.00 EUR

Ham's F-12K (Kaighn's) Medium ist eine Modifizierung der Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium wurde entwickelt für primäreWeitere Informationen

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Katalognummer	Menge
21127022	500 mL
21127030	10 x 500 mL

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Katalognummer 21127022

Preis (EUR)

54,00

Each

Menge:

500 mL

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Preis (EUR)

54,00

Each

Ham's F-12K (Kaighn's) Medium ist eine Modifizierung der Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium wurde entwickelt für primäre humane Hepatozyten sowie für einige Ratten- und Huhn-Leberzellen in einer serumreduzierten Umgebung.

Dieses F-12K (Kaighn's) Medium wird wie folgt hergestellt:

Mit	Ohne
• L-Glutamin	• HEPES
• Phenolrot

Die vollständige Zusammensetzung ist verfügbar.

Verwendung von Ham's F-12K
Ham's F-12K (Kaighn's) Medium enthält viele Komponenten, die in herkömmlichen Basalmedien nicht enthalten sind, beispielsweise Putrescin, Thymidin, Hypoxanthin, Zink sowie höhere Mengen aller Aminosäuren und Natriumpyruvat. Dank dieser Zugaben kann das Medium für gewisse Zelltypen mit sehr niedrigen Mengen von Serum oder definierten Komponenten ergänzt werden. Ham's F-12K (Kaighn's) Medium enthält keine Proteine oder Wachstumsfaktoren. Daher werden häufig Wachstumsfaktoren und fötales Rinderserum (FBS) beigesetzt. Die FBS-Konzentration muss für jede Zelllinie optimiert werden. Ham's F-12K (Kaighn's) Medium verwendet ein Puffersystem mit Natriumbikarbonat-(2,5 g/l) und erfordert daher eine 5 bis 10%ige CO₂-Umgebung, um den physiologischen pH-Wert aufrechtzuerhalten.

cGMP-konforme Herstellung an zwei Standorten
Um eine kontinuierliche Versorgung zu gewährleisten, produzieren wir Ham's F-12K (Kaighn's) Medium an zwei separaten Standorten in Grand Island, NY, USA, und Schottland, Großbritannien. Beide Standorte erfüllen die cGMP-Fertigungsanforderungen, sind zertifiziert nach ISO 13485 und bei der FDA als Hersteller medizinischer Geräte registriert.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZelltypHumane Hepatozyten

Konzentration1 X

Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard

ProduktlinieGibco

ProdukttypHam's F-12K (Kaighn's) Medium

Menge500 mL

Haltbarkeit12 Monate ab Herstellungsdatum

VersandbedingungRaumtemperatur

KlassifikationOhne Stoffe tierischen Ursprungs

FormFlüssig

SterilitätSteril gefiltert

Sterilization MethodSterile-filtered

Mit AdditivenGeringe Glukosekonzentration, Glutamin, Phenolrot, Natriumpyruvat

Ohne AdditiveKeine HEPES

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen: 2 bis 8 °C. Vor Licht schützen
Versandbedingungen: Umgebungstemperatur

Häufig gestellte Fragen (FAQ)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Ham's F-12K (Kaighn's) Medium FAQs

Zitierungen und Referenzen (5)

Zitierungen und Referenzen

Abstract

Human MutY homolog, a DNA glycosylase involved in base excision repair, physically and functionally interacts with mismatch repair proteins human MutS homolog 2/human MutS homolog 6.

Authors: Gu Yesong; Parker Antony; Wilson Teresa M; Bai Haibo; Chang Dau-Yin; Lu A-Lien;

Journal:J Biol Chem

PubMed ID:11801590

Adenines mismatched with guanines or 7,8-dihydro-8-oxo-deoxyguanines that arise through DNA replication errors can be repaired by either base excision repair or mismatch repair. The human MutY homolog (hMYH), a DNA glycosylase, removes adenines from these mismatches. Human MutS homologs, hMSH2/hMSH6 (hMutSalpha), bind to the mismatches and initiate the repair on ... More

Stimulation of neuronal neurite outgrowth using functionalized carbon nanotubes.

Authors:Matsumoto K, Sato C, Naka Y, Whitby R, Shimizu N

Journal:Nanotechnology

PubMed ID:20173239

Low concentrations (0.11-1.7 microg ml(-1)) of functionalized carbon nanotubes (CNTs), which are multi-walled CNTs modified by amino groups, when added with nerve growth factor (NGF), promoted outgrowth of neuronal neurites in dorsal root ganglion (DRG) neurons and rat pheochromocytoma cell line PC12h cells in culture media. The quantity of active ... More

Dystrophin deficiency markedly increases enterovirus-induced cardiomyopathy: a genetic predisposition to viral heart disease.

Authors: Xiong Dingding; Lee Gil-Hwan; Badorff Cornel; Dorner Andrea; Lee Sang; Wolf Paul; Knowlton Kirk U;

Journal:Nat Med

PubMed ID:12118246

Both enteroviral infection of the heart and mutations in the dystrophin gene can cause cardiomyopathy. Little is known, however, about the interaction between genetic and acquired forms of cardiomyopathy. We previously demonstrated that the enteroviral protease 2A cleaves dystrophin; therefore, we hypothesized that dystrophin deficiency would predispose to enterovirus-induced cardiomyopathy. ... More

The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain.

Authors: Kuroda Taruho S; Fukuda Mitsunori; Ariga Hiroyoshi; Mikoshiba Katsuhiko;

Journal:J Biol Chem

PubMed ID:11773082

rab27A, which encodes a small GTP-binding protein, was recently identified as a gene in which mutations caused human hemophagocytic syndrome (Griscelli syndrome) and ashen mice, which exhibit defects in melanosome transport as well as in regulated granule exocytosis in cytotoxic T lymphocytes. However, little is known about the molecular mechanism ... More

Role for 18:1 lysophosphatidic acid as an autocrine mediator in prostate cancer cells.

Authors:Xie Y, Gibbs TC, Mukhin YV, Meier KE.

Journal:J Biol Chem

PubMed ID:12084719

Lysophosphatidic acid (LPA) is a lipid mediator that may play an important role in growth and survival of carcinomas. In this study, LPA production and response were characterized in two human prostate cancer (CaP) cell lines: PC-3 and Du145. Bombesin, a neuroendocrine peptide that is mitogenic for CaP cells, stimulated ... More