Applied Biosystems™

TaqMan™ GMO Maize 35S Detection Kit, with protocol

This kit contains reagents needed to reliably detect the presence of genetically modified organism (GMO)-specific DNA sequences in seed, grain,Weitere Informationen

Katalognummer	Menge
4327693	1 Kit, with protocol

Katalognummer 4327693

Preis (EUR)

1.318,00

Menge:

1 Kit, with protocol

Preis (EUR)

1.318,00

This kit contains reagents needed to reliably detect the presence of genetically modified organism (GMO)-specific DNA sequences in seed, grain, processed foods and their ingredients.

• Universal assay detects and quantifies all approved genetically modified organisms (GMOs) in Europe and the vast majority of GMOs approved in other countries
• Fluorogenic 5' nuclease assay using TaqMan™ probes provides accurate and reproducible quantitative results
• Label license conveys the PCR service rights for GMO testing when used in conjunction with an Authorized Thermal Cycler, so no additional royalties are payable
• Comprehensive controls provide high confidence in results
• Innovative GMO quantitation software allows users to quickly and easily determine the percentage of GMO content
• Offers the complete solution including sample preparation, instruments, reagents, analysis software, technical support, instrument service, and GMO expertise

The Complete Solution
The TaqMan™ GMO Detection and Quantitation Kit is the first of a full product line of GMO assays in development that can detect the presence of GMO-specific DNA sequences in seed, grain, and processed foods and their ingredients. The TaqMan™ GMO kit is part of a highly sophisticated but user-friendly system that includes DNA sample preparation, automated PCR amplification and signal detection, data analysis, and quantitation software, thus eliminating the need for personnel trained in molecular techniques.

Reliable TaqMan™ Chemistry
The system uses the patented fluorogenic 5' nuclease method for multiplex detection and quantitation, the latest innovation in real-time PCR technology. It targets the Cauliflower Mosaic Virus 35S promoter, a GMO-specific sequence present in all GMO soy and maize events approved for use in food by the European Union, and in the vast majority of GMO events approved by other countries. Both the European Union and Japan view this method as the premier technology for quantitating GMO content in food. Other TaqMan™ kits are available for the detection and quantitation of food-borne pathogens, including Salmonella and E. coli 0157:H7.

For Research Use Only. Not for use in diagnostics procedures.

Nur für Forschungszwecke.Darf nicht für diagnostische Verfahren eingesetzt werden.

Specifications

Zur Verwendung mit (Geräte)7300 System, 7500 System, AB 7900 HT 384-Well System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode

Hot-StartIntegrierter Heißstart

Anzahl Reaktionen100 Reaktionen

Passiver ReferenzfarbstoffROX (vorgemischt)

PolymeraseAmpliTaq Gold DNA-Polymerase

ProduktlinieTaqMan

ProdukttypGMO Maize 35S Detection Kit

Menge1 Kit, with protocol

ProbentypFood, Seed

Klasse des ZielorganismusMaize, Genetically Modified

NachweisverfahrenPrimer-Sonde

GC-Rich PCR PerformanceHoch

PCR-MethodeqPCR

ReaktionsgeschwindigkeitStandard

Unit SizeEach

Inhalt und Lagerung

Each kit contains TaqMan GMO Mix, AmpliTaq Gold™ DNA Polymerase, positive and negative controls. A protocol is also included. Each kit contains enough regents for 100 assays.

Store all components at -25 to -15°C.

Häufig gestellte Fragen (FAQ)

How can food samples be prepared for TaqMan GMO analysis?

PrepMan Ultra Sample Preparation Reagent (P/N 4322547) is a good choice for food sample preparation.

How are the GMO (genetically modified organisms) reference standards (manufactured by the Institute of Reference Materials and Measurements) made?

To manufacture the standards, GMO flour is blended with non-GMO flour to obtain specific percentage GMO content, in the range of 0% to 5%. In addition, these standards can serve as a template preparation control since the template for the assay is prepared directly from the soy or maize flour.

Are GMO maize or GMO soy reference standards included in the TaqMan GMO Maize 35S (P/N 4327693) and the TaqMan GMO Soy 35S (P/N 4327692) kits?

No, reference standards are not provided in these kits. The standards are manufactured by the Institute of Reference Materials and Measurements (IRMM), and are available through distributors such as Fluka and Resource Technology Corporation.

How does one calculate the amount of genetically modified organisms (GMO) present in a food sample?

The TaqMan GMO Detection Kits are multiplex assays that detect and quantify a GMO-specific target and a plant-specific target. The amount of GMO soy or GMO maize is calculated as a percent of the total maize or soy in any given food. The endogenous reference target provides a means for determining the total amount of maize or soy in the sample. Including the appropriate GMO standard permits accurate determination for the percent of GMO content in each sample. The absence of a signal from the endogenous target, indicating the presence of PCR inhibitors in the sample, signifies a false negative result. For more information please consult the TaqMan GMO Detection Kit User Guide.

What target sequences do the TaqMan GMO (genetically modified organisms) kits detect?

In both of the kits: the TaqMan GMO Maize 35S (P/N 4327693) and the TaqMan GMO Soy 35S (P/N 4327692) Detection Kits, there is a GMO-specific target and a specific endogenous reference (ER) target. The GMO-specific target for both kits is the Cauliflower Mosaic Virus (CMV) 35S promoter. The ER target is a maize- or soy-specific locus. The ER target acts both as an internal positive control and as a means for determining the total amount of maize or soy in the sample. For more information please consult the TaqMan GMO Detection Kit User Guide.