MicroSEQ™ 500 16S rDNA Sequencing Kit

Zitierungen und Referenzen (159)

Applied Biosystems™

MicroSEQ™ 500 16S rDNA Sequencing Kit

The MicroSEQ™ 500 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System,Weitere Informationen

Katalognummer	Menge
4346480	1 Kit

Katalognummer 4346480

Preis (EUR)

936,00

Zum Warenkorb hinzufügen

Menge:

1 Kit

Preis (EUR)

936,00

Zum Warenkorb hinzufügen

The MicroSEQ™ 500 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use this kit to sequence PCR products that have been generated using the MicroSEQ™ 500 16S rDNA PCR Kit. The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene. Sequences can then be compared to the MicroSEQ™ 16S rDNA 500 Library to generate an identity match. This kit does not include a protocol or a Quick Reference Card.

• Sequence-based measurement ensures you get reproducible results in under 24 hours.
• Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ™ ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training-same work flow as the MicroSEQ™ D2 LSU fungal kit.

The MicroSEQ™ ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSeq bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

NachweisverfahrenCapillary Electrophoresis

Zur Verwendung mit (Anwendung)Sequenzierung

Zur Verwendung mit (Geräte)310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer

EnthältForward sequencing mix (2 tubes), and reverse sequencing mix (2 tubes)

BibliothekenCustomizable, Bacterial Library (> 2000 species)

Anzahl Reaktionen55 Vorwärts, 55 Rückwärts

ProduktlinieMicroSEQ

ProdukttypSequenzierungskit

Menge1 Kit

ProbentypPCR-Produkt

VersandbedingungTrockeneis

SoftwareMicroSEQ™ ID Analysis Software

Ziel1st 500 bp of 16S rRNA gene

Klasse des ZielorganismusBakterien

VerfahrenSBT (Sequence-Based Typing)

ArbeitsablaufschrittCycle Sequencing

FormatKit

Unit SizeEach

Inhalt und Lagerung

Each kit contains:
Two tubes each of forward and reverse sequencing mix. Sufficient reagent to perform 55 forward and 55 reverse sequencing reactions. Store at -15 to -25°C.

Häufig gestellte Fragen (FAQ)

Which thermocyclers are compatible with the MicroSEQ 500 16S rDNA Sequencing Kit?

To take advantage of the reduced amplification and sequencing times allowed by the new fast PCR chemistry and the 9800 Fast Thermal Cycler, we recommend using the Applied Biosystems 9800 Fast Thermal Cycler with the MicroSEQ kits. The GeneAmp PCR System 9700 may also be used but note that the 9700 thermocycler does not provide the reduced amplification and sequencing times allowed by the new fast PCR chemistry and the 9800 Fast Thermal Cycler.

Do you have stability information for the MicroSEQ 500 16S rDNA Sequencing Kit (Cat. No. 4346480) when subjected to temperature fluctuations?

The MicroSEQ 500 16S rDNA Sequencing Kit is stable if stored at -20 degrees C for 15-16 months. We do not have any information available for temperatures outside of this range.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Is the MicroSEQ 500 16S rDNA Sequencing Kit (Cat. No. 4346480) FDA approved?

No, it is for RUO.

Zitierungen und Referenzen (159)

Zitierungen und Referenzen

Abstract

Structures of aldouronic acids liberated from kenaf xylan by endoxylanases from Streptomyces sp.

Authors:Komiyama, H; Enomoto, A; Sueyoshi, Y; Nishio, T; Kato, A; Ishii, T; Shimizu, K

Journal:

PubMed ID:

'Streptomyces sp. SK519 was cultivated in the medium containing 0.5% birch wood xylan to produce extracellular xylanase. The xylanase was isolated from the culture supernatant by ammonium sulfate precipitation and partially purified by absorbing on the insoluble xylan. Alkali-extracted kenaf glucuronoylan of which main chain primarily consisting of (1-4)-linked β-D-xylopyranose ... More

Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site

Authors:Gupta, SK; Kumari, R; Prakash, O; Lal, R

Journal:

PubMed ID:

'A Gram-negative, motile, rod-shaped, non-sporulating, aerobic bacterial strain (Esp-1(T)) was isolated from oil-contaminated soil of Panipat Oil Refinery, India, and its taxonomic position was determined using a polyphasic approach. Strain Esp-1(T) grew in the presence of 2% NaCl at 30 &DEG;C and was characterized chemotaxonomically by having C(16:0) as the ... More

Multilocus DNA sequence comparisons rapidly identify pathogenic molds.

Authors:Rakeman JL; Bui U; Lafe K; Chen YC; Honeycutt RJ; Cookson BT

Journal:

PubMed ID:

'The increasing incidence of opportunistic fungal infections necessitates rapid and accurate identification of the associated fungi to facilitate optimal patient treatment. Traditional phenotype-based identification methods utilized in clinical laboratories rely on the production and recognition of reproductive structures, making identification difficult or impossible when these structures are not observed. We ... More

Species distribution and antimicrobial resistance of enterococci isolated from surface and ocean water

Authors:Moore, DF; Guzman, JA; Mcgee, C

Journal:

PubMed ID:

'Aims: The species identification and antimicrobial resistance profiles were determined for enterococci isolated from Southern California surface and ocean waters. Methods and Results: Species identification was determined for 1413 presumptive Enterococcus isolates from urban runoff, bay, ocean and sewage water samples. The most frequently isolated species were Enterococcus faecalis, Enterococcus ... More

Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification card.

Authors:Hata DJ; Hall L; Fothergill AW; Larone DH; Wengenack NL

Journal:

PubMed ID:

'The performance of the new VITEK 2 Advanced Colorimetry yeast identification (YST) card for use with the VITEK 2 system (bioMerieux, Inc., Hazelwood, MO) was compared to that of the API 20C AUX (API) system (bioMerieux SA, Marcy-l''Etoile, France) in a multicenter evaluation. A total of 12 quality control, 64 ... More

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