Applied Biosystems™

Megaplex™ PreAmp Primers, Human Pool A v2.1

Für den Einsatz in Fällen, in denen die Empfindlichkeit des Assays von größter Wichtigkeit ist, verbessern die Megaplex™ PreAmp PrimerWeitere Informationen

Katalognummer	Menge
4399233	50 Reaktionen

Katalognummer 4399233

Preis (EUR)

990,00

Menge:

50 Reaktionen

Preis (EUR)

990,00

Für den Einsatz in Fällen, in denen die Empfindlichkeit des Assays von größter Wichtigkeit ist, verbessern die Megaplex™ PreAmp Primer die Fähigkeit, niedrig exprimierte microRNAs zu erkennen, was die Erstellung eines umfassenden Expressionsprofils mit einer viel kleineren Probenmenge ermöglicht, d. h. mit nur 1 ng der verwendeten Gesamt-RNA.Mit Inhalten, die auf die Megaplex™ RT Primer, Megaplex™ PreAmp Primer abgestimmt sind, ist Human-Pool A v2.1 für die Verwendung mit Megaplex™ RT Primern, Human-Pool A v2.1 ausgelegt.Nach der Reverse Transkription mit Megaplex™ RT Primern verstärken Megaplex™ PreAmp Primer zusammen mit dem TaqMan® PreAmp Master Mix alle microRNAs vor der Quantifizierung durch Echtzeit-PCR mit TaqMan® Array MicroRNA Cards.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Anzahl Reaktionen50 Reaktionen

PrimerPreAmp-Primer

ProduktlinieMegaplex, TaqMan

ProdukttypPreamp-Primer

Menge50 Reaktionen

VersandbedingungRaumtemperatur

SpeziesHuman

Ausreichend für50 Reaktionen

GC-Rich PCR PerformanceNiedrig

PCR-MethodeqPCR

Unit SizeEach

Inhalt und Lagerung

Bei -20 °C lagern.

Häufig gestellte Fragen (FAQ)

I want to purchase TaqMan Array Human MicroRNA A Cards v2.0 (Cat. No. 4398965). What other reagents do I need to purchase in addition to these cards?

In order to perform reverse transcription on these cards, you will need the following:

TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)
Megaplex RT Primers pools (for human Type A cards Cat. No. 4399966, for Type B cards Cat. No. 4444281)

You can proceed with or without a preamplification step which depends on the total RNA amount. Total RNA amount of 1‐1000 ng supports reverse transcription reaction with preamplification, whereas 350‐1000 ng of total RNA supports a reaction without preamplification. In case you are performing preamplification you would also need the TaqMan PreAmp Master Mix (Cat. No. 4391128) and the Megaplex PreAmp Primers, Human Pool A v2.1 (Cat. No. 4399233). You will also need the Master Mix for the qPCR reaction. It is not included in the card kit. The recommended Master Mix is: TaqMan Fast Advanced Master Mix – run mode Fast (Cat. No. 4444556) For further information, please see the following link.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

View all Megaplex™ PreAmp Primers, Human Pool A v2.1 FAQs