Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Applied Biosystems™

Path-ID™ Multiplex One-Step Kit

Das Path-ID™ Multiplex One-Step RT PCR Kit wurde für eine quantitative, Reverse-Transkription-PCR (qRT-PCR) mit Multiplex entwickelt.Es ist optimiert für dieWeitere Informationen
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KatalognummerAnzahl Reaktionen
4428207500 Reaktionen
4428206100 Reaktionen
44400221000 Reaktionen
Katalognummer 4428207
Preis (EUR)
1.754,00
Each
Anzahl Reaktionen:
500 Reaktionen
Preis (EUR)
1.754,00
Each
Das Path-ID™ Multiplex One-Step RT PCR Kit wurde für eine quantitative, Reverse-Transkription-PCR (qRT-PCR) mit Multiplex entwickelt.Es ist optimiert für die Amplifikation von bis zu vier Zielen gleichzeitig mit Ihren RNA-Proben und TaqMan® Primer⁄Sondensätzen.Die Reaktionen werden in einem einstufigen Verfahren mit Einzelröhrchen durchgeführt, um die RNA umzuschreiben und Ihre Targets zu verstärken.Das Kit enthält ein Multiplex-Enzymgemisch mit Arrayscript™ Reverse Transkriptase und AmpliTaq Gold® DNA-Polymerase sowie einen Puffermix.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Zur Verwendung mit (Geräte)7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well-System, StepOne™, Standardmodus, StepOnePlus™, Standardmodus
FormatRöhrchen
Hot-StartIntegrierter Heißstart
Anzahl Reaktionen500 Reaktionen
Passiver ReferenzfarbstoffROX (vorgemischt)
PolymeraseAmpliTaq Gold DNA-Polymerase
ProduktliniePath-ID
ProdukttypOne-Step RT-PCR-Kit
Menge500 reactions
Reverse TranskriptaseArrayScript™
ProbentypGesamt-RNA, virale RNA, mRNA
Ausreichend für500 Reaktionen
NachweisverfahrenPrimer-Sonde
Zur Verwendung mit (Anwendung)Pathogennachweis, Virus Detection
FormFlüssig
PCR-Methode1-step RT-qPCR, Multiplex qPCR
ReaktionsgeschwindigkeitStandard
Unit SizeEach
Inhalt und Lagerung
•7 mL of 2X Multiplex RT-PCR Buffer
• 1.4 mL of 10X Multiplex Enzyme Mix including AmpliTaq Gold and ArrayScript
• 25 mL of Nuclease-free water
• Store the kit at -20°C; the Nuclease-free water may be stored at -20°C, 4°C, or room temperature

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Häufig gestellte Fragen (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.