Megaplex™ PreAmp Primers, Human Pool Set v3.0

Wenn die Assay-Empfindlichkeit von größter Wichtigkeit ist, verbessern Megaplex™ PreAmp-Primer die Fähigkeit der Erkennung niedrig exprimierter microRNAs deutlich, was dieWeitere Informationen

Katalognummer	Menge
4444748	50 reactions

Katalognummer 4444748

Preis (EUR)

1.138,00

Zum Warenkorb hinzufügen

Menge:

50 reactions

Preis (EUR)

1.138,00

Zum Warenkorb hinzufügen

Wenn die Assay-Empfindlichkeit von größter Wichtigkeit ist, verbessern Megaplex™ PreAmp-Primer die Fähigkeit der Erkennung niedrig exprimierter microRNAs deutlich, was die Erstellung eines umfassenden Expressionsprofils mit einer viel kleineren Probenmenge von nur 1 ng der eingesetzten Gesamt-RNA ermöglicht.Mit Inhalten, die auf die Megaplex™ RT-Primer, Megaplex™ PreAmp-Primer abgestimmt sind, wird Human-Poolset v3.0 mit Megaplex™ RT-Primern, Human-Poolset v3.0 verwendet.Nach der Reverse Transkription mit Megaplex™ RT Primern verstärken Megaplex™ PreAmp Primer zusammen mit dem TaqMan® PreAmp Master Mix alle microRNAs vor der Quantifizierung durch Echtzeit-PCR mit TaqMan® Array MicroRNA Cards.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Anzahl Reaktionen50 Reaktionen

PrimerPreAmp-Primer

ProduktlinieMegaplex

ProdukttypPreamp-Primer

Menge50 reactions

SpeziesHuman

Ausreichend für50 Reaktionen

GC-Rich PCR PerformanceNiedrig

PCR-MethodeqPCR

Unit SizeEach

Inhalt und Lagerung

Aufbewahrung bei -20 °C

Häufig gestellte Fragen (FAQ)

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

View all Megaplex™ PreAmp Primers, Human Pool Set v3.0 FAQs