Zitierungen und Referenzen (6)

Invitrogen™

Zufällige Primer

Diese absolut zufälligen Primer sind geeignet für die DNA-Synthese, bei der Klenow-Fragmente mit DNA-Matrizen verwendet werden oder für die cDNA-Synthese unter Verwendung von Reverser Transkriptase mit mRNA-Matrizen

Katalognummer	Menge
48190011	100 μl

Katalognummer 48190011

Preis (EUR)

222,00

Menge:

100 μl

Preis (EUR)

222,00

Random-Primer sind Oligodesoxyribonukleotide (meist Hexamere), die zur Herstellung markierter DNA-Sonden von Vorlagen für die Filter- oder In-situ-Hybridisierung und zum Priming von mRNAs mit oder ohne poly(A) für die cDNA-Synthese eingesetzt werden. Diese absolut zufälligen Primer sind geeignet für die DNA-Synthese, bei der Klenow-Fragmente mit DNA-Vorlagen verwendet werden, oder für die cDNA-Synthese unter Verwendung von reverser Transkriptase mit mRNA-Vorlagen. Zur Vermeidung von Speicherpuffer-Interferenzen werden zufällige Primer in einem Puffer mit geringer Salzkonzentration geliefert.

Leistungs- und Qualitätstests:
Die Aufnahme eines radioaktiv markierten Nukleotids wird mit Hilfe von Kontroll-DNA in einer Markierungsreaktion mit zufälligen Primern verifiziert.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Zur Verwendung mit (Anwendung)cDNA-Synthese

FormFlüssig

ProdukttypPrimer

Menge100 μl

VersandbedingungZugelassen für den Versand auf Nass- oder Trockeneis

Konzentration3 μg/μL

PrimerZufallssequenzen

Unit SizeEach

Inhalt und Lagerung

100 µl zufälligen Primern werden bei einer Konzentration von 3 µg/µl in 3 mM Tris-HCl (pH 7,0), 0,2 mM EDTA geliefert. Bei -20 °C lagern. Bei ordnungsgemäßer Lagerung garantiert 6 Monate stabil.

Häufig gestellte Fragen (FAQ)

Can I purchase Random Primers separately?

Yes, we do offer Random Primers as a stand-alone item: Cat. No. 48190011.

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

What is the concentration of Random Primers (Cat. No. 48190011)?

The concentration of Random Primers (Cat. No. 48190011) is 1500 pmol/µL or 1.5 mM.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

Zitierungen und Referenzen (6)

Zitierungen und Referenzen

Abstract

Global Expression Profiling of Acetate-grown Escherichia coli.

Authors: Oh Min-Kyu; Rohlin Lars; Kao Katy C; Liao James C;

Journal:J Biol Chem

PubMed ID:11815613

'This study characterized the transcript profile of Escherichia coli in acetate cultures using DNA microarray on glass slides. Glucose-grown cultures were used as a reference. At the 95% confidence level, 354 genes were up-regulated in acetate, while 370 genes were down-regulated compared with the glucose-grown culture. Generally, more metabolic genes ... More

RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs.

Authors:Ebinu JO, Bottorff DA, Chan EY, Stang SL, Dunn RJ, Stone JC

Journal:Science

PubMed ID:9582122

RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of EF hands that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling ... More

Class II histone deacetylases act as signal-responsive repressors of cardiac hypertrophy.

Authors:Zhang CL, McKinsey TA, Chang S, Antos CL, Hill JA, Olson EN,

Journal:Cell

PubMed ID:12202037

The heart responds to stress signals by hypertrophic growth, which is accompanied by activation of the MEF2 transcription factor and reprogramming of cardiac gene expression. We show here that class II histone deacetylases (HDACs), which repress MEF2 activity, are substrates for a stress-responsive kinase specific for conserved serines that regulate ... More

Improvements in siRNA properties mediated by 2'-deoxy-2'-fluoro-beta-D-arabinonucleic acid (FANA).

Authors:Dowler T, Bergeron D, Tedeschi AL, Paquet L, Ferrari N, Damha MJ,

Journal:Nucleic Acids Res

PubMed ID:16554553

RNA interference (RNAi) has emerged recently as an efficient mechanism for specific gene silencing. Short double-stranded small interfering RNAs (siRNAs) are now widely used for cellular or drug target validation; however, their use for silencing clinically relevant genes in a therapeutic setting remains problematic because of their unfavourable metabolic stability ... More

Use of an in vivo reporter assay to test for transcriptional and translational fidelity in yeast.

Authors: Shaw Randal J; Bonawitz Nicholas D; Reines Daniel;

Journal:J Biol Chem

PubMed ID:12006589

Eukaryotic RNA polymerase II and Escherichia coli RNA polymerase possess an intrinsic ribonuclease activity that is stimulated by the polymerase-binding proteins SII and GreB, respectively. This factor-activated hydrolysis of nascent RNA has been postulated to be involved in transcription elongation as well as removal of incorrect bases misincorporated into RNA. ... More

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