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Halt™ Protease und Phosphatase Inhibitor Cocktail, EDTA-frei (100x)
Halt™ Protease und Phosphatase Inhibitor Cocktail, EDTA-frei (100x)
Halt™ Protease und Phosphatase Inhibitor Cocktail, EDTA-frei (100x)
Zitierungen und Referenzen (5)
Thermo Scientific™

Halt™ Protease und Phosphatase Inhibitor Cocktail, EDTA-frei (100x)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-frei (100X), bietet die Vorteile einer einzelnen Lösung mit vollem Proteinschutz fürWeitere Informationen
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KatalognummerMenge
7844324 x 100 μl
784411 ml
784455 x 1 ml
7844710 ml
Katalognummer 78443
Preis (EUR)
414,65
Offre exceptionnelle en ligne
466,00
Ersparnis 51,35 (11%)
Each
Menge:
24 x 100 μl
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
414,65
Offre exceptionnelle en ligne
466,00
Ersparnis 51,35 (11%)
Each
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-frei (100X), bietet die Vorteile einer einzelnen Lösung mit vollem Proteinschutz für Zell- und Gewebelysate.

Merkmale des Halt Protease and Phosphatase Inhibitor Cocktails, EDTA-frei (100X):

• Vollständiger Schutz: Komplett-Cocktail enthält Protease- und Phosphatase-Inhibitoren
• Mehrere Packungsgrößen: Zur Auswahl stehen vier praktische Packungsgrößen für nahezu jede Größenordnung
• Praktisch : 100X-Flüssigformulierung ist viel einfacher und effektiver zu verwenden als einzelne Inhibitoren und andere Formate
• Problemlose Lagerung: Wasserbasiertes Format ermöglicht eine bequeme Lagerung im Kühlraum ohne Einfrieren, im Gegensatz zu DMSO-basierten Cocktails
• Kompatibel: Kann in der Massenspektrometrie (MS) verwendet werden, da es kein AEBSF enthält, das zu einer Verschiebung der Peaks führen kann
• Validiert: Voll kompatibel mit Thermo Scientific Pierce Cell Lysis Puffern

Halt Protease and Phosphatase Inhibitor Cocktail ist speziell optimiert, um Proteine vor der Zersetzung während der Extraktion und Aufreinigung zu schützen. Der Cocktail enthält Inhibitoren gegen die wichtigsten Proteasen und Phosphatasen: Aminopeptidasen, Cystein- und Serin-Proteasen sowie Serin/Threonin- und Protein-Tyrosin-Phosphatasen.

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Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
PufferLysepuffer
BeschreibungHalt Protease- und Phosphatase-Inhibitor-Cocktail, EDTA-frei
HemmstoffeProtease-Inhibitor, Phosphatase-Inhibitor
Menge24 x 100 μl
ReagenztypProtease-Inhibitor, Phosphatase-Inhibitor
FormFlüssig
ProduktlinieHalt
ProdukttypInhibitorcocktail
Unit SizeEach
Inhalt und Lagerung
Nach Erhalt bei 4 °C lagern

Häufig gestellte Fragen (FAQ)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease und Phosphatase Inhibitor Cocktail, EDTA-frei (100x) FAQs

Zitierungen und Referenzen (5)

Zitierungen und Referenzen
Abstract
Proteomic profiling across breast cancer cell lines and models.
Authors:Kalocsay M,Berberich MJ,Everley RA,Nariya MK,Chung M,Gaudio B,Victor C,Bradshaw GA,Eisert RJ,Hafner M,Sorger PK,Mills CE,Subramanian K
Journal:Scientific data
PubMed ID:37542042
We performed quantitative proteomics on 60 human-derived breast cancer cell line models to a depth of ~13,000 proteins. The resulting high-throughput datasets were assessed for quality and reproducibility. We used the datasets to identify and characterize the subtypes of breast cancer and showed that they conform to known transcriptional subtypes, ... More
Sting and p53 DNA repair pathways are compromised in Alzheimer's disease.
Authors:Nelson TJ,Xu Y
Journal:Scientific reports
PubMed ID:37221295
Alzheimer's disease (AD) is the most common cause of dementia. A common finding in AD is DNA damage. Double-strand DNA breaks (DSBs) are particularly hazardous to neurons because their post-mitotic state forces neurons to rely on error-prone and potentially mutagenic mechanisms to repair DNA breaks. However, it remains unclear whether ... More
FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.
Authors:Bartley CM,O'Keefe RA,Bordey A
Journal:PloS one
PubMed ID:24806451
Hyperactive mammalian target of rapamycin (mTOR) is associated with cognitive deficits in several neurological disorders including tuberous sclerosis complex (TSC). The phosphorylation of the mRNA-binding protein FMRP reportedly depends on mTOR complex 1 (mTORC1) activity via p70 S6 kinase 1 (S6K1). Because this phosphorylation is thought to regulate the translation ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts.
Authors:Id Boufker H, Lagneaux L, Najar M, Piccart M, Ghanem G, Body JJ, Journé F
Journal:BMC Cancer
PubMed ID:20565769
The proto-oncogene Src is an important non-receptor protein tyrosine kinase involved in signaling pathways that control cell adhesion, growth, migration and differentiation. It negatively regulates osteoblast activity, and, as such, its inhibition is a potential means to prevent bone loss. Dasatinib is a new dual Src/Bcr-Abl tyrosine kinase inhibitor initially ... More