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Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Zitierungen und Referenzen (7)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Der Thermo Scientific Halt Protease- und Phosphatase-Inhibitor-Cocktail (100X) bietet den Komfort einer einzigen Lösung mit vollem Schutz der Proteinprobe fürWeitere Informationen
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KatalognummerMenge
784445 x 1 ml
7844224 x 100 μl
7844610 ml
784401 ml
Katalognummer 78444
Preis (EUR)
729,65
Online Exclusive
802,00
Ersparnis 72,35 (9%)
Each
Menge:
5 x 1 ml
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
729,65
Online Exclusive
802,00
Ersparnis 72,35 (9%)
Each
Der Thermo Scientific Halt Protease- und Phosphatase-Inhibitor-Cocktail (100X) bietet den Komfort einer einzigen Lösung mit vollem Schutz der Proteinprobe für Zell- und Gewebelysate.

Merkmale des Halt Protease- und Phosphatase-Inhibitor-Cocktails (100X):

• Vollständiger Schutz: Komplett-Cocktail enthält Protease- und Phosphatase-Inhibitoren
• Mehrere Packungsgrößen: Zur Auswahl stehen vier praktische Packungsgrößen für nahezu jede Größenordnung
• Praktisch : 100X-Flüssigformulierung ist viel einfacher und effektiver zu verwenden als einzelne Inhibitoren und andere Formate
• Problemlose Lagerung: Wasserbasiertes Format ermöglicht eine bequeme Lagerung im Kühlraum ohne Einfrieren, im Gegensatz zu DMSO-basierten Cocktails
• Kompatibel: Kann in der Massenspektrometrie (MS) verwendet werden, da es kein AEBSF enthält, das zu einer Verschiebung der Peaks führen kann
• Validiert: Voll kompatibel mit Thermo Scientific Pierce Cell Lysis Puffern

Halt Protease and Phosphatase Inhibitor Cocktail ist speziell optimiert, um Proteine vor der Zersetzung während der Extraktion und Aufreinigung zu schützen. Er enthält Inhibitoren gegen die wichtigsten Protease- und Phosphatase-Klassen und wirkt gezielt gegen Aminopeptidasen, Cystein- und Serin-Proteasen sowie gegen Serin/Threonin- und Protein-Tyrosin-Phosphatasen. Um die Hemmung der Metalloproteasen zu fördern, wird 0,5 M EDTA in einem separaten Röhrchen geliefert.

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Halt™ Protease- und Phosphatase-Inhibitor-Cocktail, EDTA-frei (100X)
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
PufferLysepuffer
HemmstoffeProtease-Inhibitor, Phosphatase-Inhibitor
Menge5 x 1 ml
ReagenztypProtease-Inhibitor, Phosphatase-Inhibitor
FormFlüssig
ProduktlinieHalt
ProdukttypInhibitorcocktail
Unit SizeEach
Inhalt und Lagerung
Nach Erhalt bei 4 °C lagern

Häufig gestellte Fragen (FAQ)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Cocktail (100X) FAQs

Zitierungen und Referenzen (7)

Zitierungen und Referenzen
Abstract
Host E3 ubiquitin ligase ITCH mediates Toxoplasma gondii effector GRA35-triggered NLRP1 inflammasome activation and cell-autonomous immunity.
Authors:Wang Y,Hollingsworth LR,Sangaré LO,Paredes-Santos TC,Krishnamurthy S,Penn BH,Wu H,Saeij JPJ
Journal:mBio
PubMed ID:38376248
Toxoplasma gondii is an intracellular parasite that can activate the NLRP1 inflammasome leading to macrophage pyroptosis in Lewis rats, but the underlying mechanism is not well understood. In this study, we performed a genome-wide CRISPR screen and identified the dense granule proteins GRA35, GRA42, and GRA43 as the Toxoplasma effectors ... More
LARP1 binds ribosomes and TOP mRNAs in repressed complexes.
Authors:Saba JA,Huang Z,Schole KL,Ye X,Bhatt SD,Li Y,Timp W,Cheng J,Green R
Journal:The EMBO journal
PubMed ID:39533057
Terminal oligopyrimidine motif-containing mRNAs (TOPs) encode all ribosomal proteins in mammals and are regulated to tune ribosome synthesis to cell state. Previous studies have implicated LARP1 in 40S- or 80S-ribosome complexes that are thought to repress and stabilize TOPs. However, a molecular understanding of how LARP1 and TOPs interact with ... More
A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions.
Authors:Moissoglu K,Wang T,Gasparski AN,Stueland M,Paine EL,Jenkins LM,Mili S
Journal:Cell reports
PubMed ID:39982819
mRNA localization to subcellular compartments is a widely used mechanism that functionally contributes to numerous processes. mRNA targeting can be achieved upon recognition of RNA cargo by molecular motors. However, our molecular understanding of how this is accomplished is limited, especially in higher organisms. We focus on a pathway that ... More
hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels.
Authors:Gibson A, Lewis AP, Affleck K, Aitken AJ, Meldrum E, Thompson N
Journal:J Biol Chem
PubMed ID:15919655
'Proteins of the CLCA gene family have been proposed to mediate calcium-activated chloride currents. In this study, we used detailed bioinformatics analysis and found that no transmembrane domains are predicted in hCLCA1 or mCLCA3 (Gob-5). Further analysis suggested that they are globular proteins containing domains that are likely to be ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
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