Nitrocellulose Membranes, 0.2 μm
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Thermo Scientific™

Nitrocellulose Membranes, 0.2 μm

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods).
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KatalognummerDimensions (LxW)Ausreichend für
880248 x 8 cm15 Minigel-Blots
880137,9 cm x 10,5 cm15 Minigel-Blots
770128 x 12 cm25 Minigel-Blots
Katalognummer 88024
Preis (EUR)
183,00
Each
Zum Warenkorb hinzufügen
Dimensions (LxW):
8 x 8 cm
Ausreichend für:
15 Minigel-Blots
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
183,00
Each
Zum Warenkorb hinzufügen
Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.2-μm pore size is well-suited for western blotting, and this smaller pore size is ideal for the transfer of low molecular weight proteins or peptides and nucleic acids (<300 bp).

Features include:
100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background—produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Bindungsvermögen∼209 μg/cm2 of protein
BeschreibungPierce Nitrozellulose-Membran, 0.2 um, 8 cm x 8 m
Menge15 Blatt
VersandbedingungRaumtemperatur
Dimensions (LxW)8 x 8 cm
FormatBlatt
Länge (metrisch)8 cm
MaterialCellulosenitrat
Porengröße0.2 μm
Ausreichend für15 Minigel-Blots
Breite (metrisch)8 cm
Unit SizeEach
Inhalt und Lagerung
Lagern Sie die Membranen flach bei Umgebungstemperatur, fern von chemischen Dämpfen. Einige Lösungsmitteldämpfe können die Membranen teilweise auflösen, wodurch die Porenstruktur gestört wird. Die Membranen keinem direkten Sonnenlicht aussetzen. Sonnenlicht kann dazu führen, dass die Nitrozellulose trocknet und spröde wird.
Hinweis: Nitrozellulose-Membranfilter sind leicht entzündlich. Von Wärmequellen und offenem Feuer fernhalten. Der Flammpunkt liegt bei ca. 2 °C. Die Membranen können mit Dampf autoklavierbar bei 121 °°C sterilisiert werden.

Häufig gestellte Fragen (FAQ)

How can I store, strip, and reuse my western blot?

For nitrocellulose or PVDF membrane following Western blot detection using a chemiluminescent or fluorescent substrate system: Following transfer, air dry the membrane and place in an envelope, preferably on top of a supported surface to keep the membrane flat. The blot can be stored indefinitely at -80 degrees C. When ready to reprobe, prewet the PVDF blot with alcohol for a few seconds, followed by a few rinses with pure water to reduce the alcohol concentration. Then proceed as normal with blocking step.

FOR STRIPPING/REPROBING OF MEMBRANES: Harsh protocol (see NOTE below for modifications)

1) Submerge the membrane in stripping buffer (100 mM BME, 2% SDS, 62.5 mM Tris-HCl, pH 6.7) and incubate at 50 degrees C for 30 min with occasional agitation. If more stringent conditions necessary, incubate at 70 degrees C.

2) Wash 2 x 10 min in TBS-T/PBS-T at room temperature.

3) Block the membrane by immersing in 5% blocking reagent TBS-T or PBS-T for 1 hr at room temperature.

4) Immunodetection

NOTE: Often you don't need such harsh conditions to remove antibodies from their proteins. The stringency of one or several of the variables can be decreased: lower the temperature, decrease the time, less BME, less SDS, etc. An especially mild but still often effective stripping protocol is lower pH incubation. Example: pH 2.0 Tris 50-100 mM, 30-60 min incubation (you may do two incubations if you wish). Then rinse and block as usual. If you do not wish to re-use the membrane immediately after stripping, you can store the membrane in plastic wrap (wet, you do not want it to dry out). Another simple, mild stripping buffer is 0.1 M glycine•HCl (pH 2.5-3.0), incubation 30 min to 2 hrs room temperature or 37 degrees C, depending on the antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are your PVDF and nitrocellulose membranes compatible with the Li-COR instrument?

Yes, both our PVDF and nitrocellulose membranes are compatible with the Li-COR instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.