GeneChip™ miRNA 3.0 Array
Applied Biosystems™

GeneChip™ miRNA 3.0 Array

Many diseases, including cancer, are frequently described as diseases of disordered gene expression. It is estimated that more than 30%Weitere Informationen
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KatalognummerMenge
90201930 Arrays
Katalognummer 902019
Preis (EUR)
12.470,00
Each
Menge:
30 Arrays
Preis (EUR)
12.470,00
Each
Many diseases, including cancer, are frequently described as diseases of disordered gene expression. It is estimated that more than 30% of protein translation of coding genes is regulated by miRNA. There is also a large amount of growing evidence suggesting miRNA interacts with long non-coding RNA in the signaling networks that regulate alternative splicing events, which impacts cellular processes such as apoptosis, proliferation, and differentiation– all of which have shown to be causative elements in diseases such as cancer.

Measuring the changes in these critical nodes of regulation is extremely important for deciphering the biological context of differentially expressed genes. This new array design is a powerful tool for studying the role of small non-coding RNAs and their involvement in a broad spectrum of developmental and physiological mechanisms.

To keep pace with the discovery of new and novel miRNA, we are pleased to offer the GeneChip™ miRNA 3.0 Array to our growing catalog of miRNA arrays.

GeneChip miRNA 3.0 Arrays help bring you closer to biology with:
Comprehensive coverage – Designed to interrogate all mature miRNA sequences in miRBase Release 20
Easily correlate miRNA results – Analysis files contain host gene ID, predicted and validated miRNA target genes, and clustered miRNA information
Easy analysis – Analyze human, mouse, rat, or every miRNA for all species using the same array
Low sample input – Requires as little as 130 ng total RNA
Simple, fast, and free analysis solution – Coupled with GeneChip Expression Console and Transcriptome Analysis Console (TAC) Software, researchers have a complete solution from data to decision-making in minutes

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications
ProduktlinieGeneChip
Menge30 Arrays
TypmiRNA 3.0 Array
ArraymiRNA Profiling
FormatArray Cartridge
Anzahl Arrays30 arrays
SpeziesHuman, Mouse, Rat
Unit SizeEach

Häufig gestellte Fragen (FAQ)

Where can I find the species information for your GeneChip miRNA arrays?

The species information can be found in the annotation file of the miRNA array of interest.
Please complete the following steps:
- Go to www.thermofisher.com and go to the product page of the array of interest.
- Scroll down to support files.
- Download the annotation files for the miRNA array of interest, and open in excel.
- In the file, there will be a column, species scientific name. This column will have all the species present on the array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

For GeneChip miRNA arrays, are External RNA Controls Consortium (ERCC) controls tiled on the array?

The ERCC controls are not tiled onto GeneChip miRNA arrays because they would not work with the miRNA array/assay system. The FlashTag Biotin HSR RNA Labeling Kits used to label miRNAs is optimized for shortRNAs. Even though longer mRNAs like the ERCC controls will be labeled, the HWS (hyb/wash/stain) conditions are not optimal and consequently, the longer mRNAs typically do not get detected.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Which of the gene arrays are compatible with the miRNA interaction network in Transcriptome Analysis Console (TAC) Software?

The miRNA interaction network in TAC Software is available for all gene arrays, including model organisms. To have the interaction network available, the same samples must have been processed through both the gene array and the miRNA array. Additionally, the gene array being used must have a gene symbol column displayed in their annotation.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How does data compare between GeneChip miRNA 3.0 Array cartridges and GeneChip miRNA 3.1 Array Strips?

GeneChip miRNA 3.1 Array Strips provide a linear dynamic range (> 3 log10) that is similar to that provided by the GeneChip miRNA 3.0 Array cartridges. When evaluating non-control probe sets that are common to both arrays, we observed very high signal correlation (Pearson correlation coefficients of 0.97 - 0.98 for signal correlation) and fold change value correlation (Pearson correlation coefficients of 0.95 - 0.97 for fold change value correlation) for GeneChip miRNA 3.0 Array cartridges compared to GeneChip miRNA 3.1 Array Strip.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Was the dynamic range (3 logs) for the GeneChip miRNA 3.0 Arrays (described in a table for the miRNA 3.0 array data sheet) estimated or calculated in the same way as described in a much more comprehensive GeneAtlas, GeneTitan, GCS3000 platform comparison?

For the miRNA 3.0 array data, we used 16 spiked miRNA transcripts and measured linearity of median spike signal over a broad range of concentration, similar to the platform comparison document. So Fig. 3 in the tech note, linked here: http://media.affymetrix.com/support/technical/technotes/mirna2_array_technote.pdf, shows the signal with spikes at 0.1, 1, 10 and 100 attomoles/100 ng.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.