Clariom™ S Array, human
Clariom™ S Array, human
Applied Biosystems™

Clariom™ S Array, human

Obtain a gene-level view of the human transcriptome with Clariom S Assays HT for human samples. Clariom S Assays serveWeitere Informationen
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KatalognummerMenge
9029601 x 24-Array-Platte
Katalognummer 902960
Preis (EUR)
3.690,00
Each
Zum Warenkorb hinzufügen
Menge:
1 x 24-Array-Platte
Preis (EUR)
3.690,00
Each
Zum Warenkorb hinzufügen
Obtain a gene-level view of the human transcriptome with Clariom S Assays HT for human samples. Clariom S Assays serve as a next generation transcriptome-wide gene-level expression profiling tool, which allows for the fastest, simplest, and most scalable path to generating the results you need for your research. Based on industry-leading microarray technology, the novel Human HT Clariom S Assay design provides extensive coverage of all known well-annotated genes, compatibility with research sample types, scalable formats, and flexible data analysis software. Clariom S Assays HT are the tools of choice to find expression biomarkers with known function as quickly, easily, and cost-effectively as possible.

Find answers, move on
Although the number of known transcribed genes has expanded rapidly in recent years, knowledge of the function of each gene is still evolving. Many genes and transcripts found in databases are poorly annotated or unannotated, which can complicate and prolong data analysis and interpretation. Human HT Clariom S Assays focus on well-annotated genes, providing researchers with the ability to perform gene-level expression profiling studies and to quickly assess changes in key genes and pathways. With less time required for data analysis, Clariom S Assays HT for human help researchers reach conclusions more rapidly.

Simple, swift biomarker discovery
• Accurately measure gene-level expression from >20,000 well-annotated genes to get to answers quickly.
• Choose a format that suits your throughput needs, processing from 1 to 192 samples a day.
• Go from data to insight in minutes with intuitive, highly visual, free analysis software designed for the biologist.

When you have precious samples, get it right the first time
• Generate robust expression profiles from as little as 100 pg of total RNA—as few as 10 cells.
• Utilize RNA from various sample types including blood, cells, and fresh/fresh-frozen or FFPE tissues.
• Preserve sample integrity and reduce data variability with an assay that does not require a globin or rRNA removal step.
• Save time and money with fully automated sample preparation options.

Clariom S solutions are available formats for single-sample (cartridge array) processing on the GeneChip™ 3000 instrument system and high-throughput automated processing (plate array) on the GeneTitan™ HT Microarray System, offering the flexibility to accommodate both small and large cohort studies. The complete solution comes with reagents and fast, simple Transcriptome Analysis Console (TAC) software to analyze and visualize global expression patterns of genes, pathways, and network interactions in minutes.

Get the truest level of gene-level expression
To generate robust gene-level expression, Human HT Clariom S Assays detect only the exons present in all known transcript isoforms expressed from a single gene locus-constitutive exon. This differs from other gene-level array technologies and shallow RNA-Seq, which provide either a biased view of gene expression or data that are complicated by variation in expression of transcript variants. By detecting only constitutive exons throughout the length of each known gene, Human HT Clariom S Assays generate the most accurate and truest measurement of gene-level expression available today.

Keeping biomarker identification across the transcriptome simple and swift, Clariom S Assays HT for human provide you with the coverage you require, the reproducibility you need, and the insights you want to act on your discoveries.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications
EnthältClariom S 24-Array Plate
ProduktlinieApplied Biosystems
Menge1 x 24-Array-Platte
VersandbedingungApproved for shipment at Room Temperature or on Wet or Dry Ice
TypS Array
ArrayTranscriptome Profiling
Format24-array Plate
Anzahl Arrays24 Arrays
SpeziesHuman
Unit SizeEach
Inhalt und Lagerung
Clariom S 24-Array Plate, human, store at 4°C

Häufig gestellte Fragen (FAQ)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why is it important to use Tough-Spots® labels when using GeneChip cartridge arrays?

Tough-Spots® labels are small adhesive stickers used to temporarily seal the backs of cartridge arrays during the overnight hybridization step. They are required to prevent loss of volume due to evaporation through the septa. We recommend using Tough-Spots® labels on Rolls from USA Scientific (Item No. 9185-0000)

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What are the respective hybridization volumes based on array format for Affymetrix gene expression arrays?

Proper hybridization volume is critical to obtaining an even signal across a given array. Too little volume can lead to black circles in the middle of the array. Too much volume can leak out of the back of the array. The correct hybridization volume leaves enough room for a small air bubble to circulate around the array surface during the overnight hybridization. Here are the recommended hybridization and fill volumes based on the array format:
Array Format; Hybridization Volume; Fill Volume
- 49 Format (Standard); 200 µL; 250 µL
- 64 Format; 200 µL; 250 µL
- 100 Format (Midi); 130 µL; 160 µL
- 169 Format (Mini); 80 µL; 100 µL
- 400 Format (Micro); 80 µL; 100µL

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How many data sources were used for the Clariom S array?

We used 15 databases to design the Clariom S array. These databases are listed in the Clariom S solutions data sheet that can be found on the Clariom S assay, mouse product page, under Documents > Product Literature: https://www.thermofisher.com/order/catalog/product/902930?SID=srch-srp-902930#/902930.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why do Clariom S arrays use the (IVT) Pico kit, but Clariom D arrays use the WT Pico kit for challenging samples?

The GeneChip WT Pico kit is a strand-specific reagent, which must be used with Clariom D (and HTA 2.0) to obtain strand-specific information as these arrays having probes on regions overlapping from both strands. Strand-specificity is important for identifying antisense transcripts, determining the transcribed strand of ncRNAs, and demarcating the boundaries of closely situated or overlapping genes.
Strand-specificity is less important for gene-level expression analysis (i.e., when using Clariom S). Probes within regions of overlapping transcription from both strands are avoided in the Clariom S array design; i.e., the array is "stranded" meaning it does not necessarily need a strand-specific reagent kit.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.