StemPro™ Chondrogenese-Differenzierungs-Kit

Zitierungen und Referenzen (2)

Gibco™

StemPro™ Chondrogenese-Differenzierungs-Kit

Katalognummer	Menge
A1007101	1 Kit

Katalognummer A1007101

Preis (EUR)

208,65

Online Exclusive

229,00

Ersparnis 20,35 (9%)

Menge:

1 Kit

Preis (EUR)

208,65

Online Exclusive

229,00

Ersparnis 20,35 (9%)

Das StemPro® Chondrogenesis Differentiation Kit wurde für die chondrogenetische Differenzierung von mesenchymalen Stammzellen (MSCs) in Gewebekulturgefäßen entwickelt. Das Kit enthält alle erforderlichen Reagenzien, um die MSC dazu anzuregen, der Chondrogenese-Signalkaskade zu folgen und Chondrozyten zu generieren. Die Verwendung des StemPro® Chondrogenesis Differentiation Kits in Kombination mit StemPro® MSC SFM oder MesenPRO RS™ Medium bietet eine standardisierte Kultur-Workflow-Lösung für die Isolierung, Expansion und Differenzierung von MSCs in Collagenmatrix-produzierende Chondrozyten.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZelltypStammzellen (mesenchymal)

KulturmilieuCO2

Endotoxin-Gehalt≤ 50 EU/ml, ≤ 50 EU/ml

FormatFlüssig

GlucoseGeringe Glukosekonzentration

GlutaminGlutaMAX™-I

HEPES-PufferKeine HEPES

Phenolrot-IndikatorKein Phenolrot

Reinheits- oder QualitätsgradForschungsqualität

Menge1 Kit

ForschungskategorieDifferenzierung

Serumspiegelserumfrei

NatriumbicarbonatpufferNatriumhydrogencarbonat

Natriumpyruvat-AdditivNatriumpyruvat

SpeziesHuman

Zur Verwendung mit (Anwendung)Differenzierung von Stammzellen

ProduktlinieGibco, StemPro

ProdukttypChondrogenese-Differenzierungskit

Unit SizeEach

Inhalt und Lagerung

Dieses Kit wird mit 100 ml Basismedium und 10 ml Wachstumssupplement geliefert, die für die Formulierung von 100 ml des vollständigen Mediums geeignet sind. Die Komponenten erfordern unterschiedliche Versand- und Lagerungsbedingungen. Das Wachstumssupplement ist nicht einzeln erhältlich.
StemPro™ Osteocyten⁄Chondrocyten-Differenzierungsbasismedium bei 2 bis 8 °C (vor Licht schützen)
StemPro™ Chondrogenese-Supplement bei -5 bis -20 °C (im Dunkeln)

Häufig gestellte Fragen (FAQ)

How many times can I freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A1006401) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?

We do not recommend that you freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A10064-01) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101). Addition of supplement to the StemPro Osteocyte/Chondrocyte Differentiation Basal Medium (Cat. No. A1006901) is stable at 2-8 degrees C for up to 1 month, protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What mesenchymal stem cells (MSC) sources can I use with the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?

The StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101) was designed and tested using human adipose-derived stem cells (ADSC). Based on protocols in literature, it should also work with mesenchymal stem cells (MSC) from other sources, including umbilical cord and cord blood.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The StemPro Chondrogenesis Differentiation Kit manual refers to MSC Attachment Medium under Chondrogenesis Differentiation, Step 7. Is MSC Attachment Medium the same as MSC growth medium?

Yes, MSC Attachment Medium is the same as MSC growth medium.

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

View all StemPro™ Chondrogenese-Differenzierungs-Kit FAQs

Zitierungen und Referenzen (2)

Zitierungen und Referenzen

Abstract

Mice lacking Nf1 in osteochondroprogenitor cells display skeletal dysplasia similar to patients with neurofibromatosis type I.

Authors:Wang W, Nyman JS, Ono K, Stevenson DA, Yang X, Elefteriou F,

Journal:Hum Mol Genet

PubMed ID:21757497

Mutations in NF1 cause neurofibromatosis type I (NF1), a disorder characterized, among other clinical manifestations, by generalized and focal bony lesions. Dystrophic scoliosis and tibial pseudoarthrosis are the most severe skeletal manifestations for which treatment is not satisfactory, emphasizing the dearth of knowledge related to the biology of NF1 in ... More

Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.

Authors:Fernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,

Journal:Tissue Eng Part C Methods

PubMed ID:30412046

cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More