Das tfernrot-fluoreszierende Alexa Fluor® 647 Alkin reagiert mit Aziden über eine kupferkatalysierte Click-Reaktion. Der helle und lichtbeständige Fluorophor kann beiWeitere Informationen
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Katalognummer
Menge
A10278
0,5 mg
Katalognummer A10278
Preis (EUR)
474,00
Each
Menge:
0,5 mg
Preis (EUR)
474,00
Each
Das tfernrot-fluoreszierende Alexa Fluor® 647 Alkin reagiert mit Aziden über eine kupferkatalysierte Click-Reaktion. Der helle und lichtbeständige Fluorophor kann bei Durchflusszytometrie, Mikroskopie und HCS verwendet werden
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Chemische ReaktivitätAzid
NachweisverfahrenFluoreszent
FormatFest
Marker oder FarbstoffAlexa Fluor™ 647
ProdukttypAlkyn
Menge0,5 mg
Reaktive GruppeAlkyn
Reaktiver TeilAlkyn
VersandbedingungRaumtemperatur
LöslichkeitDMSO (Dimethylsulfoxid)
FarbeFernrot
MarkertypAlexa Fluor Farbstoffe
ProduktlinieAlexa Fluor, Molecular Probes
Unit SizeEach
Inhalt und Lagerung
Bei ≤-20 °°C trocken und vor Licht geschützt lagern.
Zitierungen und Referenzen (4)
Zitierungen und Referenzen
Abstract
Dynamic monitoring of newly synthesized proteomes: up-regulation of myristoylated protein kinase A during butyric acid induced apoptosis.
Authors:Liu K, Yang PY, Na Z, Yao SQ,
Journal:Angew Chem Int Ed Engl
PubMed ID:21678537
Doubly charged: A double metabolic incorporation approach capable of proteome-wide profiling of post-translational modification dynamics on newly synthesized proteins has been developed (see scheme; blue box: methionine surrogate, orange diamond: PTM probe). This strategy reveals for the first time that up-regulation of myristoylated PKA protein is necessary for the occurrence ... More
Identification of a novel prenyl and palmitoyl modification at the CaaX motif of Cdc42 that regulates RhoGDI binding.
Authors:Nishimura A, Linder ME,
Journal:Mol Cell Biol
PubMed ID:23358418
Membrane localization of Rho GTPases is essential for their biological functions and is dictated in part by a series of posttranslational modifications at a carboxyl-terminal CaaX motif: prenylation at cysteine, proteolysis of the aaX tripeptide, and carboxymethylation. The fidelity and variability of these CaaX processing steps are uncertain. The brain-specific ... More
A multifunctional bioconjugate module for versatile photoaffinity labeling and click chemistry of RNA.
Authors:Kellner S, Seidu-Larry S, Burhenne J, Motorin Y, Helm M,
Journal:Nucleic Acids Res
PubMed ID:21646334
A multifunctional reagent based on a coumarin scaffold was developed for derivatization of naive RNA. The alkylating agent N3BC [7-azido-4-(bromomethyl)coumarin], obtained by Pechmann condensation, is selective for uridine. N3BC and its RNA conjugates are pre-fluorophores which permits controlled modular and stepwise RNA derivatization. The success of RNA alkylation by N3BC ... More
Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry.
Authors:Winz ML, Samanta A, Benzinger D, Jäschke A,
Journal:Nucleic Acids Res
PubMed ID:22344697
The modification of RNA with fluorophores, affinity tags and reactive moieties is of enormous utility for studying RNA localization, structure and dynamics as well as diverse biological phenomena involving RNA as an interacting partner. Here we report a labeling approach in which the RNA of interest--of either synthetic or biological ... More