Amplex™ Red Monoamine Oxidase Assay Kit
Amplex™ Red Monoamine Oxidase Assay Kit
Zitierungen und Referenzen (10)
Invitrogen™

Amplex™ Red Monoamine Oxidase Assay Kit

Das Amplex Red Monoaminoxidase-Assaykit bietet ein empfindliches und einfaches fluorometrisches Verfahren zum Nachweis der Monoaminoxidase-Aktivität in gereinigten Enzympräparaten mit KonzentrationenWeitere Informationen
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KatalognummerMenge
A12214500 Assays
Katalognummer A12214
Preis (EUR)
620,00
Each
Zum Warenkorb hinzufügen
Menge:
500 Assays
Preis (EUR)
620,00
Each
Zum Warenkorb hinzufügen
Das Amplex Red Monoaminoxidase-Assaykit bietet ein empfindliches und einfaches fluorometrisches Verfahren zum Nachweis der Monoaminoxidase-Aktivität in gereinigten Enzympräparaten mit Konzentrationen von nur 12 µU/ml und in Gewebeproben mit nur 20 µg Protein in einem Assay-Volumen von 100 µl.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
NachweisverfahrenFluoreszent
FarbstofftypAndere Etiketten oder Farbstoffe
FormatKüvetten, 96-Well-Platte
Menge500 Assays
VersandbedingungRaumtemperatur
Zur Verwendung mit (Anwendung)Monoaminoxidaseassay
Zur Verwendung mit (Geräte)Fluorometer, Mikrotiterplatten-Lesegerät
ProduktlinieAmplex
ProdukttypRotes Monoaminoxidaseassay-Kit
Unit SizeEach
Inhalt und Lagerung
In einem Tiefkühlgerät (-5 bis -30 °C) lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (10)

Zitierungen und Referenzen
Abstract
High-throughput screening for monoamine oxidase-A and monoamine oxidase-B inhibitors using one-step fluorescence assay.
Authors:Guang HM, Du GH
Journal:Acta Pharmacol Sin
PubMed ID:16723097
'AIM: To develop high-throughput screening (HTS) assays for monoamine oxidase (MAO)-A and MAO-B inhibitors. METHODS: A fluorescence probe based method measuring MAO-A and MAO-B activity was established and optimized, with its sensitivity, stability and specificity evaluated. Reaction conditions including enzyme sources, substrate concentrations, incubation volume and reaction time in 384-well ... More
Catecholamines regulate the activity, secretion, and synthesis of renalase.
Authors:Li G, Xu J, Wang P, Velazquez H, Li Y, Wu Y, Desir GV,
Journal:Circulation
PubMed ID:18299506
'BACKGROUND: We previously identified renalase, a secreted novel amine oxidase that specifically degrades circulating catecholamines. Parenteral administration of either native or recombinant renalase lowers blood pressure, heart rate, and cardiac contractility by metabolizing circulating catecholamines. Renalase plasma levels are markedly reduced in patients with chronic kidney disease. It is not ... More
The PPARgamma agonist pioglitazone is effective in the MPTP mouse model of Parkinson's disease through inhibition of monoamine oxidase B.
Authors:Quinn LP, Crook B, Hows ME, Vidgeon-Hart M, Chapman H, Upton N, Medhurst AD, Virley DJ,
Journal:Br J Pharmacol
PubMed ID:18332857
'BACKGROUND AND PURPOSE: The peroxisome proliferator-activated receptor-gamma (PPARgamma) agonist pioglitazone has previously been shown to attenuate dopaminergic cell loss in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson''s disease, an effect attributed to its anti-inflammatory properties. In the present investigation, we provide evidence that pioglitazone is effective in the MPTP mouse ... More
Renalase lowers ambulatory blood pressure by metabolizing circulating adrenaline.
Authors:Desir GV, Tang L, Wang P, Li G, Sampaio-Maia B, Quelhas-Santos J, Pestana M, Velazquez H,
Journal:J Am Heart Assoc
PubMed ID:23130169
'Blood pressure is acutely regulated by the sympathetic nervous system through the action of vasoactive hormones such as epinephrine, norepinephrine, and dopamine. Renalase, a recently described, secreted flavoprotein, acutely decreases systemic pressure when administered in vivo. Single-nucleotide polymorphisms present in the gene are associated with hypertension, cardiac disease, and diabetes. ... More
Quantitative structure-activity relationship and complex network approach to monoamine oxidase A and B inhibitors.
Authors:Santana L, González-Díaz H, Quezada E, Uriarte E, Yáñez M, Viña D, Orallo F,
Journal:J Med Chem
PubMed ID:18834112
'The work provides a new model for the prediction of the MAO-A and -B inhibitor activity by the use of combined complex networks and QSAR methodologies. On the basis of the obtained model, we prepared and assayed 33 coumarin derivatives, and the theoretical prediction was compared with the experimental activity ... More
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