Biocytin Alexa Fluor™ 488 (Alexa Fluor™ 488 Biocytin, Disodium Salt)

Zitierungen und Referenzen (9)

Invitrogen™

Biocytin Alexa Fluor™ 488 (Alexa Fluor™ 488 Biocytin, Disodium Salt)

Die zellundurchlässige, fixierbare, polare Markierungssubstanz Alexa Fluor™ 488 Biocytin kombiniert das grün fluoreszierende Alexa Fluor™ 488 Fluorophor mit Biotin undWeitere Informationen

Katalognummer	Menge
A12924	250 μg

Katalognummer A12924

Preis (EUR)

504,00

Zum Warenkorb hinzufügen

Menge:

250 μg

Preis (EUR)

504,00

Zum Warenkorb hinzufügen

Die zellundurchlässige, fixierbare, polare Markierungssubstanz Alexa Fluor™ 488 Biocytin kombiniert das grün fluoreszierende Alexa Fluor™ 488 Fluorophor mit Biotin und einem aldehydfixierbaren primären Amin. Polare Tracer werden häufig zur Untersuchung der Zelle-zu-Zelle- und Zellliposomenfusion sowie der Membrandurchlässigkeit und des Transports durch Gap Junctions oder Zellaufnahmen während der Pinozytose verwendet. Dieser wasserlösliche Tracer kann mittels der Whole-Cell-Patch-Clamp-Technik, Iontophorese, osmotischer Lyse von pinozytischen Vesikeln oder vergleichbare Methoden in die Zellen eingebracht werden. Das Biotin ermöglicht den Nachweis und die anschließende Amplifikation mit enzymmodifizierten Streptavidinen einschließlich Meerrettich (HRP) und alkalischer Phosphatase (AP), die mit TSA- bzw. ELF-Technologien verwendet werden können.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Marker oder FarbstoffAlexa Fluor Farbstoffe

Menge250 μg

VersandbedingungRaumtemperatur

ProduktlinieAlexa Fluor

Unit SizeEach

Inhalt und Lagerung

Lagerung bei Raumtemperatur und vor Licht geschützt

Häufig gestellte Fragen (FAQ)

I injected a fluorescent tracer, but cannot detect it after tissue is fixed and sectioned. What am I doing wrong?

Confirm that the tracer you are using crosslinks to proteins or has a primary amine for fixation-either a hydrazide, lysine fixable dextran, or a protein conjugate.
Use aldehyde-based fixatives to cross link the amines on the tracer.
Inject a larger amount or higher concentration of the tracer. Tracers are generally injected at 1-20% concentrations (10 mg/mL or higher).
Confirm that you are using the correct fluorescent filter for detection. You can perform a spot test by pipetting a small amount of the undiluted stock solution of the tracer onto a slide, then view under the filter you are using on your microscope. This will confirm if the tracer fluorescence can be detected and the fluorescent microscope filter is working properly.
Review tissue fixation and handling procedures to confirm if any reagents or processing procedures could be affecting the tracer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have labeled my neurons with an Alexa Fluor conjugated biocytin to look at transport but I wanted to examine only retrograde transport and biocytin appears to be moving retrograde and anterograde. What should I do?

Observing both types of transport is typical for biocytin. The conjugated cholera toxin subunit B products have been observed to travel only retrogradely.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have a neuronal tracer similar to Lucifer Yellow but in another fluorescent color?

Lucifer Yellow CH is a hydrazide, so any of our Alexa Fluor or fluorescent hydrazides could potentially be used. A listing of them can be found here. (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing/hydrazides-biocytins.html#prd)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (9)

Zitierungen und Referenzen

Abstract

Deconvolving single-molecule intensity distributions for quantitative microscopy measurements.

Authors:Mutch SA, Fujimoto BS, Kuyper CL, Kuo JS, Bajjalieh SM, Chiu DT,

Journal:Biophys J

PubMed ID:17259276

'In fluorescence microscopy, images often contain puncta in which the fluorescent molecules are spatially clustered. This article describes a method that uses single-molecule intensity distributions to deconvolve the number of fluorophores present in fluorescent puncta as a way to "count" protein number. This method requires a determination of the correct ... More

Hebbian LTP in feed-forward inhibitory interneurons and the temporal fidelity of input discrimination.

Authors:Lamsa K, Heeroma JH, Kullmann DM

Journal:Nat Neurosci

PubMed ID:15937481

'Cortical information processing requires a delicate balance of excitatory and inhibitory signaling. How is this balance preserved during hippocampal memory encoding, which involves NMDA receptor-dependent long term potentiation (LTP)? This form of LTP occurs at synapses between pyramidal neurons but has not been detected in feed-forward inhibitory interneurons. We show ... More

Evolutionary origins for social vocalization in a vertebrate hindbrain-spinal compartment.

Authors:Bass AH, Gilland EH, Baker R,

Journal:Science

PubMed ID:18635807

'The macroevolutionary events leading to neural innovations for social communication, such as vocalization, are essentially unexplored. Many fish vocalize during female courtship and territorial defense, as do amphibians, birds, and mammals. Here, we map the neural circuitry for vocalization in larval fish and show that the vocal network develops in ... More

Electrophysiological properties of morphologically-identified medial vestibular nucleus neurons projecting to the abducens nucleus in the chick embryo.

Authors:Gottesman-Davis A, Shao M, Hirsch JC, Peusner KD,

Journal:Neuroscience

PubMed ID:20971163

'Neurons in the medial vestibular nucleus (MVN) show a wide range of axonal projection pathways, intrinsic firing properties, and responses to head movements. To determine whether MVN neurons participating in the vestibulocular reflexes (VOR) have distinctive electrophysiological properties related to their output pathways, a new preparation was devised using transverse ... More

The styryl dye FM1-43 suppresses odorant responses in a subset of olfactory neurons by blocking cyclic nucleotide-gated (CNG) channels.

Authors:Breunig E, Kludt E, Czesnik D, Schild D,

Journal:J Biol Chem

PubMed ID:21646359

'Many olfactory receptor neurons use a cAMP-dependent transduction mechanism to transduce odorants into depolarizations. This signaling cascade is characterized by a sequence of two currents: a cation current through cyclic nucleotide-gated channels followed by a chloride current through calcium-activated chloride channels. To date, it is not possible to interfere with ... More

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