Zitierungen und Referenzen (10)

Gibco™

PSC Cardiomyocyte Differentiation Kit

Katalognummer	Menge
A2921201	1 Kit

Katalognummer A2921201

Preis (EUR)

652,00

Menge:

1 Kit

Preis (EUR)

652,00

Das Gibco™ PSC Kardiomyozyten-Differenzierungskit besteht aus einem Satz Serum- und Xeno-freier Medien, die eine effiziente Differenzierung pluripotenter Human-Stammzellen (PSC) in kontrahierenden Kardiomyozyten in nur 8 Tagen ermöglichen. Im Gegensatz zu anderen Methoden, die mehrere Komponenten und eine längere Testdauer erfordern, kann das PSC Kardiomyozyten-Differenzierungskit Kardiomyozyten aus pluripotenten Stammzellen in einem gebrauchsfertigen Medienformat und in kürzerer Zeit erzeugen.

Jedes Medium besteht aus drei 1X Medien, die kein Auftauen oder Mischen erfordern und nacheinander über insgesamt 14 Tage verwendet werden. Das Ergebnis sind funktionsfähige Kardiomyozyten, die relevante physiologische Marker aufweisen, in Kultur kontrahieren und anschließend mehr als 15 Tage in Kultur erhalten werden können (Erhaltungsmedium auch separat erhältlich (Kat.- Nr. A2920801)).

Kein Auftauen oder Mischen erforderlich
Das PSC Kardiomyozyten-Differenzierungskit besteht aus 1x Medien, die keine Gefrierlagerung erfordern, sodass kein Auftauen oder Mischen von Reagenzien erforderlich ist. Erwärmen Sie einfach das Medium und fügen Sie es der PSC-Kultur bei.

Schnelle Produktion von Kardiomyozyten
Das PSC Kardiomyozyten-Differenzierungskit kann in nur 8 Tagen kontrahierende Kardiomyozyten produzieren. Differenzierte Kardiomyozyten können anschließend für mehr als 15 Tage in Kultur gehalten werden.

Erzeugung hochwertiger Kardiomyozyten
Kardiomyozyten, die mit dem PSC Kardiomyozyten-Differenzierungskit erzeugt werden, sind funktional relevant, kontrahieren in Kultur und exprimieren wichtige Marker wie TNNT2, Nkx2.5, MYH6 und α-Actinin.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZelltypKardiomyozyten

Menge1 Kit

VersandbedingungRaumtemperatur

ProdukttypPSC Cardiomyocyte Differentiation Kit

Unit SizeEach

Inhalt und Lagerung

• 500 ml Kardiomyozyten-Pflegemedium
• 100 ml Kardiomyozyten-Differenzierungsmedium
• 100 ml Kardiomyozyten-Differenzierungsmedium B

Bei 2 bis 8 °C lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

What substrate should I use for cardiomyocyte differentiation?

We recommend using Gibco Geltrex Flex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A4000046801, A4000046802, or A4000046803) or Gibco Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A400457 or A400458) for xeno-free applications.

Is StemFlex Medium compatible with downstream differentiation kits available from Thermo Fisher Scientific?

Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I used the Gibco PSC Cardiomyocyte Differentiation Kit and am not seeing good differentiation with my iPSC line. What should I do?

We recommend always using H9 or H7 ESC line as a control in your experiments. We recommend adjusting the cell density or extending the induction time for difficult-to-differentiate iPSC lines.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am planning to use the PSC Cardiomyocyte Differentiation Kit. Which markers can I expected my differentiated cells to express?

Cardiomyocytes generated using PSC Cardiomyocyte Differentiation Kit have been tested for key markers such as TNNT2, Nkx2.5, MYH6, and Alpha-Actinin. The Cardiomyocyte Immunocytochemistry Kit (Cat. No. A25973) contains validated antibodies to measure TNNT2 and Nkx2.5 in cultures generated using PSC Cardiomyocyte Differentiation Kit.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I maintain differentiated cells in culture using the Gibco PSC Cardiomyocyte Differentiation Kit?

Differentiated cells can be maintained for a month or longer for long-term studies. We recommend the use of Gibco Geltrex Matrix for long-term cultures.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all PSC Cardiomyocyte Differentiation Kit FAQs

Zitierungen und Referenzen (10)

Zitierungen und Referenzen

Abstract

Cardiac disease modeling using induced pluripotent stem cell-derived human cardiomyocytes.

Authors:Dell'Era P, Benzoni P, Crescini E, Valle M, Xia E, Consiglio A, Memo M,

Journal:

PubMed ID:25815118

Causative mutations and variants associated with cardiac diseases have been found in genes encoding cardiac ion channels, accessory proteins, cytoskeletal components, junctional proteins, and signaling molecules. In most cases the functional evaluation of the genetic alteration has been carried out by expressing the mutated proteins in in-vitro heterologous systems. While ... More

Generation of the human induced pluripotent stem cell (hiPSC) line PSMi004-A from a carrier of the KCNQ1-R594Q mutation.

Authors:Mura M, Lee YK, Pisano F, Ginevrino M, Boni M, Calabrò F, Crotti L, Valente EM, Schwartz PJ, Tse HF, Gnecchi M

Journal:Stem Cell Res

PubMed ID:30974404

'We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a male carrier of the heterozygous mutation c.1781?G?>?A p.R594Q on the KCNQ1 gene. hiPSCs, generated using four retroviruses each encoding for OCT4, SOX2, KLF4 and cMYC, display pluripotent stem cell characteristics, and can be differentiated into spontaneously beating ... More

Reprogramming of Urine-Derived Renal Epithelial Cells into iPSCs Using srRNA and Consecutive Differentiation into Beating Cardiomyocytes.

Authors:Steinle H, Weber M, Behring A, Mau-Holzmann U, von Ohle C, Popov AF, Schlensak C, Wendel HP, Avci-Adali M

Journal:Mol Ther Nucleic Acids

PubMed ID:31476669

'The generation of induced pluripotent stem cells (iPSCs) from patient''s somatic cells and the subsequent differentiation into desired cell types opens up numerous possibilities in regenerative medicine and tissue engineering. Adult cardiomyocytes have limited self-renewal capacity; thus, the efficient, safe, and clinically applicable generation of autologous cardiomyocytes is of great ... More

Targeting RyR Activity Boosts Antisense Exon 44 and 45 Skipping in Human DMD Skeletal or Cardiac Muscle Culture Models.

Authors:Barthélémy F, Wang RT, Hsu C, Douine ED, Marcantonio EE, Nelson SF, Miceli MC

Journal:Mol Ther Nucleic Acids

PubMed ID:31678734

Systemic delivery of antisense oligonucleotides (AO) for DMD exon skipping has proven effective for reframing DMD mRNA, rescuing dystrophin expression, and slowing disease progression in animal models. In humans with Duchenne muscular dystrophy treated with AOs, low levels of dystrophin have been induced, and modest slowing of disease progression has ... More

Generation of the human induced pluripotent stem cell (hiPSC) line PSMi005-A from a patient carrying the KCNQ1-R190W mutation.

Authors:Mura M, Lee YK, Pisano F, Ginevrino M, Boni M, Calabrò F, Crotti L, Valente EM, Schwartz PJ, Tse HF, Gnecchi M

Journal:Stem Cell Res

PubMed ID:31009818

We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a woman carrier of the heterozygous mutation c.568C?>?T p.R190W on the KCNQ1 gene. hiPSCs, obtained using four retroviruses enconding the reprogramming factors OCT4, SOX2, cMYC and KLF4, display pluripotent stem cell characteristics, and can be differentiated into spontaneously ... More

View all PSC Cardiomyocyte Differentiation Kit citations