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Fötales Kälberserum, ultraniedriges IgG, Herkunftsland: USA
Gibco™

Fötales Kälberserum, ultraniedriges IgG, Herkunftsland: USA

Gibco™ fötale Kälberseren bieten einen ausgezeichneten Wert für die Zellkultur, die Spezialforschung und spezifische Assays. Sie erhalten das Vertrauen vonWeitere Informationen
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KatalognummerMengeFormat
A338190150 mLOne Shot
16250078500 mLFlasche
Katalognummer A3381901
Preis (EUR)
134,00
Each
Menge:
50 mL
Format:
One Shot
Preis (EUR)
134,00
Each
Gibco™ fötale Kälberseren bieten einen ausgezeichneten Wert für die Zellkultur, die Spezialforschung und spezifische Assays. Sie erhalten das Vertrauen von Forschern mit gleichbleibender Qualität und prämierter Unterstützung, die Ihren Forschungsanforderungen und Ihrem Budget gerecht wird

Serum-Kategorie: Spezial
Herkunft: Vereinigte Staaten.
Die IgG-Konzentration liegt unter 5 µg/ml, und der BVD-Titer ist niedrig oder nicht nachweisbar.
Endotoxin-Gehalt: ≤50 EU/ml (routinemäßiger Spiegel ≤ 10 EU/ml).
Hämoglobin-Spiegel: ≤25 mg/dl.

Dieses Reagenz ist in mehreren Ländern als medizinisches Gerät klassifiziert. Wenn es in einer bestimmten Region nicht als medizinisches Gerät eingestuft wird, sollte es nur für den Laborgebrauch verwendet werden.
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
ProduktlinieOne Shot
Versandbedingunggefroren
SpeziesRind
AgeFötal
HerkunftslandVereinigte Staaten
FormFlüssig
FormatOne Shot
ProdukttypFötales Kälberserum
Menge50 mL
Serum TreatmentExosome-depletiert
SterilitätSteril
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen: ≤ -10° C
Versandbedingungen: Gefroren

Häufig gestellte Fragen (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Are there any other packaging configurations available for the new One Shot FBS product?

Yes, we also offer a 10 X 50 mL package, which is equivalent to a 500 mL bottle of FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How is the new One Shot FBS bottle different from our existing One Shot bottle?

Our previous One Shot bottle, a 60 mL Nalgene media bottle, is not designed for easy pipetting and pouring whereas the new One Shot FBS bottle has a propietary design that was developed specifically for FBS use. This design allows you to freeze, thaw and pour without the need to aliquot, which minimizes the amount of FBS handling and reduces the risk of contamination.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does the new One Shot FBS bottle fit into a conical tube rack?

The new One Shot FBS bottle is not shaped to fit into a traditional conical tube rack. It does however fit nicely in a 30 mm test tube rack for the purposes of storage and use in a water bath and biological safety cabinet.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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