Thermo Scientific™

SuperSignal™ West Atto Ultimate Sensitivity Substrat

Name: SuperSignal™ West Atto Ultimate Sensitivity Substrat
SKU: A38556
Price: 788.65 EUR

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Katalognummer	Menge
A38556	200 ml
A38554	20 ml
A38555	100 ml

3 Optionen

Katalognummer A38556

Preis (EUR)

788,65

Precio exclusivo en nuestra web

896,00

Ersparnis 107,35 (12%)

Each

Menge:

200 ml

Preis (EUR)

788,65

Precio exclusivo en nuestra web

896,00

Ersparnis 107,35 (12%)

Each

Das SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate ist ein hochempfindliches, verbessertes Chemilumineszenz(ECL)-Substrat für den Nachweis von Proteinen im hohen Attogrammbereich. Das SuperSignal West Atto Substrat bietet die höchste Empfindlichkeit und die besseren Signal-Rausch-Verhältnisse im Vergleich zu anderen handelsüblichen Hochleistungs-Meerrettich-Peroxidase(HRP)-Substraten.

Eigenschaften des SuperSignal West Atto Ultimate Sensitivity Chemilumineszenz-Substrats:
• Empfindlichkeit: Niedriges Femtogramm bis hohes Attogramm
• Stabilität: 48 Stunden für Arbeitslösung, 1 Jahr für das Kit bei 4 °C
• Kompatibilität: Nitrozellulose- und PVDF-Membranen
• Signaldauer: Bis zu 6 Stunden
• Empfohlene Konzentration des Primärantikörpers: Verdünnung 1:1.000 bis 1:5.000 (0,2 bis 1,0 µg/ml)
• Empfohlene Konzentration des Sekundärantikörpers: Verdünnung 1:100.000 bis 1:250.000 (4 bis 10 ng/ml)

Vergleichen und erfahren Sie mehr über unsere Chemilumineszenz-Substrate für Western Blot ›
Alle verfügbaren HRP-Substrate anzeigen›

Das SuperSignal West Atto Ultimate Sensitivity Chemilumineszenz-Substrat wurde für überragende Empfindlichkeit und Signal-Rausch-Verhältnisse für in sehr geringer Konzentration vorliegende Ziele oder wertvolle Proben in der Western-Blot-Analyse entwickelt. In Kombination mit optimierten Antikörperkonzentrationen und Blockierpuffern ermöglicht das SuperSignal West Atto Substrat die Detektion von Zielproteinen in Mengen, die für eine Erkennung mit herkömmlichen, hochleistungsfähigen ECL-Substraten zu gering sind.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

KitinhaltLuminol/Enhancer, 100 mL, Stable Peroxide Buffer, 100 mL

EmpfindlichkeitNiedriges Femtogramm bis hohes Attogramm

VersandbedingungNasseis

Ausreichend für25 Mini-Gel-Blots, 2.000 cm² Membran

NachweisverfahrenChemolumineszenz

Zur Verwendung mit (Anwendung)Western Blot

FormFlüssig

Membrane CompatibilityNitrozellulose, PVDF

ProduktlinieSuperSignal

ProdukttypSubstrat

Menge200 ml

SubstrateMeerrettich-Peroxidase

Unit SizeEach

Inhalt und Lagerung

• Substratlösung, 100 ml
• Stabile Peroxidlösung, 100 ml

Ausreichend für 25 Mini-Gel-Blots; 2.000 cm² Membran.

Reagenzien bei 2 bis 8 °C lagern.

Häufig gestellte Fragen (FAQ)

How do I handle saturated or burned out bands using SuperSignal West Atto Ultimate Sensitivity Substrate?

Here are some suggestions:
- Shorten the time of exposure
- Wash blot and try using SuperSignal West Pico PLUS Chemiluminescent Substrate
- Strip the membrane using Restore Western Blot Stripping Buffer, re-probe with lesser amount of primary and secondary antibodies, and try first with SuperSignal West Pico PLUS Chemiluminescent Substrate
- Re-run the gel using lesser sample

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How can I reduce background with SuperSignal West Atto Ultimate Sensitivity Substrate?

Here are potential causes for high background and corresponding solutions:
- Too much HRP in the system: Further dilute the HRP-conjugate, see guidelines in Table 1 in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018858_SuperSignalWestAtto_UG.pdf).
- Inadequate blocking: Optimize blocking conditions.
- Inappropriate blocking reagent: Try a different blocking reagent. Use clear milk or StartingBlock blocking buffer instead of dry milk.
- Inadequate washing: Increase length, number or volume of washes.
- Film has been overexposed: Decrease exposure time or use Pierce Background Eliminator (Cat No. 21065).
- Concentration of antigen or antibody is too high: Decrease amount of antigen or antibody.
- Poor antibody specificity: Use SuperSignal Western Blot Enhancer (Cat. No. 46640).
We also recommend minimizing handling of the membrane, using clean gloves while handling, clean plasticware, glassware, forceps, or other contact surfaces. We also recommend trimming of the membrane to remove excess area.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperSignal West Atto Ultimate Sensitivity Substrate compatible with Restore Western Blot Stripping Buffer?

SuperSignal West Atto Ultimate Sensitivity Substrate is compatible with Restore Western Blot Stripping Buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When should I use SuperSignal West Atto Ultimate Sensitivity Substrate?

We recommend using SuperSignal West Atto Ultimate Sensitivity Substrate for detection of proteins in the following situations:
- For proteins with low concentration, not measurable by BCA or Bradford assays
- For low-abundance phosphorylated or glycosylated proteins
- For low-abundance membrane or nuclear proteins
- For low-abundance proteins being detected with a low-affinity antibody or when the antibody is precious
- When sample source is limited or when multiple conditions or assays are being tested on a single sample

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperSignal West Atto Ultimate Sensitivity Substrate affected by freeze/thaw cycles?

SuperSignal West Atto Ultimate Sensitivity Substrate can tolerate up to 3 freeze/thaw cycles without significant loss in performance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SuperSignal™ West Atto Ultimate Sensitivity Substrate, 200 mL FAQs