PNGase F Glykan-Aufreinigungskit

Das PNGase F Glykan-Spaltungskit enthält alle Komponenten, die zur enzymatischen Entfernung fast aller N-verknüpften Oligosaccharide aus Glykoproteinen erforderlich sind. DasWeitere Informationen

Katalognummer	Menge
A39245	1 Kit

Katalognummer A39245

Preis (EUR)

323,00

Zum Warenkorb hinzufügen

Menge:

1 Kit

Preis (EUR)

323,00

Zum Warenkorb hinzufügen

Das PNGase F Glykan-Spaltungskit enthält alle Komponenten, die zur enzymatischen Entfernung fast aller N-verknüpften Oligosaccharide aus Glykoproteinen erforderlich sind. Das Kit enthält das PNGase F-Enzym, das N-Glykan-Ketten an den innersten GlcNAc- und Asparaginrückständen von Mannose-, Hybrid- und komplexen Oligosacchariden spaltet.

Eigenschaften des PNGase F Glykan-Spaltungskits:
• ≥95 % Reinheit
• Rekombinantes PNGase-F-Enzym ohne Endoglykosidase F1, F2 oder F3
• Das Enzym lässt die N-Glykan-Kernoligosaccharide intakt und eignet sich für nachfolgende Analysen
•Geeignet für die Verwendung unter nativen oder vergällenden Bedingungen

Inhalt des PNGase-F-Glykan-Spaltungskits: 30.000 Einheiten PNGase F (500.000 Einheiten/ml) und 1 ml PNGase F 10X-Puffer.

Nur für Forschungszwecke. Darf nicht für diagnostische Verfahren eingesetzt werden.

Specifications

FormFlüssig

Menge1 Kit

VersandbedingungNasseis

ProdukttypPNGase F Glykan-Spaltungskit

Unit SizeEach

Inhalt und Lagerung

• 30.000 Einheiten PNGase F-Enzym, rekombinant (500.000 Einheiten/ml), bei -20 °C
• 1 ml PNGase F 10X-Puffer, Lagerung bei -20 °C

Häufig gestellte Fragen (FAQ)

In the PNGase Glycan Cleavage Kit, can I use PNGase F under native or denaturing conditions?

Yes, PNGase F enzyme may be used in both native and denaturing conditions

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, how much PNGase F should I use to remove my carbohydrate under native or DTT denaturing conditions?

When the protein is not denatured with SDS, PNGase F has to work harder to reach the cleavage site of the carbohydrate (due to secondary and tertiary protein structures). In these instances, using more enzyme and/or extended incubation times may help. These conditions will need to be determined empirically for your specific protein.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, is PNGase F compatible with downstream analysis such as HPLC and mass spectrometry?

PNGase F enzyme is stored in 50% glycerol. Therefore, HPLC and/or mass spectrometry is not compatible due to the fact that glycerol is not tolerated in instruments used for these applications.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, what are the typical reaction conditions for PNGase F, Recombinant?

Typical reaction conditions are as follows:

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.
1. Combine 1-20 µg of glycoprotein, 1 µL of 10X denaturing buffer (5% SDS, 400 mM DTT) and H2O (if necessary) to make a 10 µL total reaction volume.
2. Denature glycoprotein by heating reaction at 100 degrees C for 10 min.
3. Make a total reaction volume of 20 µL by adding 2 µL PNGaseF 10X Buffer, 1-5 µL PNGaseF enzyme, 2 µL 10% NP-40 and H2O.
4. Incubate reaction at 37 degrees C for 1 hr.
Note: Reactions may be scaled-up linearly to accommodate larger reaction volumes.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, what happens to the asparagine residue after PNGase F removes the sugar?

As PNGase F is a glycoamidase, the asparagine gets converted into aspartic acid.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.