Gibco™

Fetal Bovine Serum, Premium Plus

Name: Fetal Bovine Serum, Premium Plus
SKU: A5669701
Price: 828.00 EUR

Premium Plus FBS, our most defined FBS, provides consistent cell growth for sensitive cell lines requiring the lowest viral risk.

Ansicht ändern

Katalognummer	Menge	Format	Hitzeinaktiviert	Gamma-irradiated
A5669701	500 mL	Flasche	Nein	Nein
A5669201	500 mL	Flasche	Ja	Ja
A5669401	50 mL	One Shot	Nein	Nein
A5669402	10 x 50 mL	One Shot	Nein	Nein
A5669501	50 mL	One Shot	Ja	Nein
A5669502	10 x 50 mL	One Shot	Ja	Nein
A5669801	500 mL	Flasche	Ja	Nein
A5669901	500 mL	Flasche	Nein	Ja

8 Optionen

Katalognummer A5669701

Preis (EUR)

828,00

Each

Menge:

500 mL

Format:

Flasche

Hitzeinaktiviert:

Nein

Gamma-irradiated:

Nein

Preis (EUR)

828,00

Each

Premium Plus FBS, our most defined FBS, provides consistent cell growth for sensitive cell lines requiring the lowest viral risk. It is ideal for use in biomanufacturing processes and diagnostic cell culture research requiring the most stringent quality profile, including an extensive biochemical and hormonal profile. Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements.

Sera category: Premium Plus

Use for a broad range of cell types, especially sensitive cell lines
Our most characterized serum with the lowest endotoxin and hemoglobin levels
Endotoxin level: ≤5 EU/mL
Hemoglobin level: ≤20 mg/dL
Origin: Refer to CoA for specific origin (US, Australia, New Zealand)
US Department of Agriculture (USDA) safety tested for the absence of bluetongue and akabane from countries that are USDA-approved for importation into the US

Gibco serum delivers

ISO 13485-certified; processed in FDA-registered facilities
Triple filtered at 0.1 μm
Gibco bottle easier to use in hood, reduces risk of contamination and helps improve consistency
Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

* FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.

Für die Verwendung in der Forschung oder die Weiterverarbeitung. Nicht zur Diagnosestellung oder direkten Anwendung bei Menschen oder Tieren geeignet.

Specifications

Endotoxin-Konzentration≤5 EU/mL

Zur Verwendung mit (Anwendung)*FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.

Gamma-irradiatedNein

GütePremium Plus

HitzeinaktiviertNein

Hämoglobin-Konzentration≤20 mg/dL

VerarbeitungTriple-filtered, 0.1μm

Haltbarkeit5 Jahre

VersandbedingungTrockeneis

SpeziesRind/Bovine

Getestet aufUp to 70 quality tests including EMA virus testing; USP/EP mycoplasma, endotoxin, performance; biochemical/hormonal profiling

USDA-Sicherheitstest enthaltenUSDA Safety Testing Performed for Mexican and Australian origins

AgeFötal

HerkunftslandInformationen zum spezifischen Ursprung finden Sie im Analysenzertifikat (CoA)*

FormGefroren

FormatFlasche

ProdukttypFetal Bovine Serum

Menge500 mL

Serum TreatmentStandard (sterilfiltriert)

SterilitätSteril

Sterilization MethodDreifach gefiltert, 0.1 μm

Unit SizeEach

Inhalt und Lagerung

Store at ≤-10°C.

Häufig gestellte Fragen (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, Premium Plus FAQs