Residual Protein TrypLE™ ELISA Kit
Gibco™

Residual Protein TrypLE™ ELISA Kit

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme.
Have Questions?
Ansicht ändernbuttonViewtableView
KatalognummerMenge
A6533103960 Tests
A653310196 Tests
A65331025 Kits, 480 Tests
Katalognummer A6533103
Preis (EUR)
-
Menge:
960 Tests

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme. The kit is compatible with all forms of TrypLE enzyme, including TrypLE Select, TrypLE Express, and CTS TrypLE Select enzymes.

TrypLE enzyme is an animal-origin-free recombinant alternative to porcine trypsin. It is used for the dissociation of attachment-dependent cell lines. TrypLE enzyme dissociates cells cultured both in serum-free and serum-supplemented systems. Due to the unique biological sequence of TrypLE enzyme, traditional trypsin ELISA kits are ineffective at binding and detecting residual levels of TrypLE enzyme.

Für die Verwendung in der Forschung oder die Weiterverarbeitung. Nicht zur Diagnosestellung oder direkten Anwendung bei Menschen oder Tieren geeignet.
Specifications
Assay-Bereich0,78 bis 50 ng/ml
Assay-Empfindlichkeit:0.032 ng/mL
KonjugatHorseradish Peroxidase (HRP)
Zur Verwendung mit (Geräte)Colorimetric Microplate Reader
FormatWash steps required
Interassay-VK6.20%
Intraassay-VK7.20%
Marker oder FarbstoffHorseradish Peroxidase (HRP)
Protein-AliaseTrypLE, R-Protease
Menge960 Tests
VersandbedingungNasseis
ZielTrypLE (R-Protease) concentration
Gesamte Assay-Zeit1.5 hr.
Immunassay-Kit-FormatSandwich-ELISA-Kit
Unit SizeEach
Inhalt und Lagerung
• Pre-coated 96-well plate
• Standard assay buffer concentrate
• HRP-conjugated detection antibody
• Wash buffer concentrate
• Chromogen stop solution
• Adhesive plate covers

Store at 2–8°C.

Häufig gestellte Fragen (FAQ)

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.