Zitierungen und Referenzen (1)

Gibco™

Human Epidermal Keratinocytes, neonatal (HEKn)

Primäre normale humane epidermale Keratinozyten, Neugeborene (HEKn) werden aus neonataler Vorhaut isoliert und am Ende der Primärkultur kryokonserviert.Die Zellen sind:•Weitere Informationen

Katalognummer	Menge
C0015C	1 Fläschchen

Katalognummer C0015C

Preis (EUR)

936,00

Menge:

1 Fläschchen

Preis (EUR)

936,00

Primäre normale humane epidermale Keratinozyten, Neugeborene (HEKn) werden aus neonataler Vorhaut isoliert und am Ende der Primärkultur kryokonserviert.

Die Zellen sind:
• Leistungsgetestet – mit mindestens 30 Populationsverdoppelungen, wenn sie in EpiLife-Medium kultiviert werden, ergänzt mit HKGS (für HEKn) oder Supplement S7 (für HEKn-AOF)
• Garantiert ≥ 70 % lebensfähig
• Geprüft für Mykoplasma, Bakterien, Hefe oder andere Pilze, Hepatitis B, Hepatitis C und HIV-1-Viren

Jedes Fläschchen enthält über 0,5 x 10⁶ lebensfähige Zellen, die am Ende der Primärkulturphase kryokonserviert wurden, in einem Medium mit 10 % DMSO.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

SpenderquelleEinzelner Spender

Anzahl Zellen≥500000

ProduktlinieCascade Biologics

ProdukttypHumane epidermale Keratinozyten

Menge1 Fläschchen

Getestet aufHepatitis B (HBV), Hepatitis C (HCV), Sterilitätstests (Bakterien & Pilze), Mycoplasma, Wachstumsleistungs-Assay, HIV-1

AgeNeugeborene (14 Tage oder weniger)

ZelltypKeratinozytenzellen

Culture TypeAdhärente Zellkultur

FormKryokonserviert

SpeziesHuman

Unit SizeEach

Inhalt und Lagerung

In Flüssigstickstoff lagern.

Häufig gestellte Fragen (FAQ)

I see large, flat pancake-looking cells in my keratinocyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.

I see large, flat pancake-looking cells in my Human Keratinodyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.

What is the doubling time for Human Keratinodyte?

Each lot is tested for doubling time and the results are listed on the COA.

Can I specify a donor type for the primary cells you offer?

This information also appears on the COA.

What is the difference between basal cells or basal keratinocytes?

These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.

View all Human Epidermal Keratinocytes, neonatal (HEKn) FAQs

Zitierungen und Referenzen (1)

Zitierungen und Referenzen

Abstract

High Sp1/Sp3 ratios in epithelial cells during epithelial differentiation and cellular transformation correlate with the activation of the HPV-16 promoter.

Authors:Apt D, Watts RM, Suske G, Bernard HU,

Journal:Virology

PubMed ID:8862423

Gene expression of human papillomavirus type 16 (HPV-16) and other HPV types is epithelial specific. Specificity is brought about by synergism between several different transcription factors that seem to occur ubiquitously but differ qualitatively and quantitatively between cells in which HPV genomes are transcriptionally active or inactive. Here, we report ... More