Zitierungen und Referenzen (2)

Gibco™

Human Umbilical Vein Endothelial Cells (HUVEC)

Primäre endotheliale Zellen der humanen Nabelschnur (HUVEC)) werden von der humanen Nabelvene isoliert und am Ende der Primärkultur kryokonserviert.Die ZellenWeitere Informationen

Katalognummer	Menge
C0035C	1 Fläschchen

Katalognummer C0035C

Preis (EUR)

588,00

Menge:

1 Fläschchen

Preis (EUR)

588,00

Primäre endotheliale Zellen der humanen Nabelschnur (HUVEC)) werden von der humanen Nabelvene isoliert und am Ende der Primärkultur kryokonserviert.

Die Zellen sind:
• Leistungsgetestet – mit mindestens 16 Populationsverdoppelungen, wenn sie in Medium 200 kultiviert werden, ergänzt mit Large Vessel Endothelial Supplement (LVES)
• Garantiert ≥ 70 % lebensfähig
• Geprüft für Mykoplasma, Bakterien, Hefe oder andere Pilze, Hepatitis B, Hepatitis C und HIV-1-Viren

Jedes Fläschchen enthält über 0,5 x 10⁶ lebensfähige Zellen, die am Ende der Primärkulturphase kryokonserviert wurden, in einem Medium mit 10 % DMSO.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

SpenderquelleEinzelner Spender

Anzahl Zellen≥500000

ProduktlinieCascade Biologics

ProdukttypEndotheliale Zellen der humanen Nabelschnur

Menge1 Fläschchen

Getestet aufHepatitis B (HBV), Hepatitis C (HCV), Sterilitätstests (Bakterien & Pilze), Mycoplasma, Wachstumsleistungs-Assay, HIV-1

AgeNeugeborene (14 Tage oder weniger)

ZelltypEndothelzellen (Nabelvene)

Culture TypeAdhärente Zellkultur

FormKryokonserviert

SpeziesHuman

Unit SizeEach

Inhalt und Lagerung

In Flüssigstickstoff lagern.

Häufig gestellte Fragen (FAQ)

What types of large vessel endothelial cells do you offer?

We carry primary human umbilical vein endothelial cells (HUVEC) from one donor or pooled from multiple donors.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

Are primary cells isolated from tissue using enzymatic digestion?

Yes, our primary cells are isolated from tissue using enzymatic digestion.

View all Human Umbilical Vein Endothelial Cells (HUVEC) FAQs

Zitierungen und Referenzen (2)

Zitierungen und Referenzen

Abstract

Generation of iPSC line epiHUVEC from human umbilical vein endothelial cells.

Authors:Matz P, Adjaye J,

Journal:

PubMed ID:26987922

Human umbilical vein endothelial cells (HUVECs) were used to generate the iPSC line epiHUVEC employing a combination of three episomal-based plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC and KLF4. Pluripotency was confirmed both in vivo and in vitro. The transcriptome profile of epiHUVEC and the human embryonic stem cell line ... More

Angiogenic properties of dehydrated human amnion/chorion allografts: therapeutic potential for soft tissue repair and regeneration.

Authors:Koob TJ, Lim JJ, Massee M, Zabek N, Rennert R, Gurtner G, Li WW,

Journal:

PubMed ID:24817999

Chronic wounds are associated with a number of deficiencies in critical wound healing processes, including growth factor signaling and neovascularization. Human-derived placental tissues are rich in regenerative cytokines and have been shown in randomized clinical trials to be effective for healing chronic wounds. In this study, PURION® Processed (MiMedx Group, ... More