CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Zitierungen und Referenzen (13)

Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Name: CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit
SKU: C10427
Price: 528.00 EUR

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Katalognummer	Menge
C10427	100 Assays
C10740	20 Assays

2 Optionen

Katalognummer C10427

Preis (EUR)

528,00

Each

Menge:

100 Assays

Preis (EUR)

528,00

Each

Das CellEvent™ Caspase-3/7 Durchflusszytometrie-Assay-Kit Grün ermöglicht die durchflusszytometrische Detektion aktivierter Caspase-3 und Caspase-7 in apoptotischen Zellen. Das Kit enthält das neuartige fluorogene Substrat CellEvent™ Caspase-3/7 Nachweisreagenz Grün sowie den SYTOX™ AADvanced™ Totzellfarbstoff.

• Caspase-3/7-spezifisch—Enthält die Erkennungssequenz für aktivierte Caspase-3 und Caspase-7
• Einfache Identifizierung—Klare Identifizierung von lebenden, toten und apoptotischen Zellpopulationen
• Schnelle Analyse—Kein Waschen und Fixieren erforderlich
•Mehrfarben-Kompatibilität—Kombinierbar mit anderen Farbstoffen, die durch den 488 nm-Laser oder andere Laser anregbar sind

CellEvent™ Caspase-3/7 Nachweisreagenz Grün ist ein zellpermeantes Reagenz, das aus einem Vier-Aminosäure-Peptid (DEVD) besteht, welches mit einem Nukleinsäure-bindenden Farbstoff konjugiert ist. Bei der Apoptose werden die Proteine Caspase-3 und Caspase-7 aktiviert und können die im DEVD-Peptid kodierte Erkennungssequenz für Caspase 3/7 spalten. Die Spaltung der Erkennungssequenz und die Bindung der DNA durch das Reagenz markiert die apoptotischen Zellen mit einem hellen, fluorogenen Signal, das ein Absorptions-/Emissionsmaximum von ca. 511/533 nm besitzt. In Verbindung mit dem SYTOX™ AADvanced™ Totzellfarbstoff können apoptotische Zellen leicht von lebenden und nekrotischen Zellen differenziert werden.

Da kein einzelner Parameter die Apoptose in allen Systemen definiert, empfehlen wir dringend, für den zuverlässigen Nachweis der Apoptose eine Kombination verschiedener Messungen zu verwenden.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Anregung/EmissionSYTOX AADvanced: 546/647
CellEvent Caspase 3/7 Nachweisreagenz Grün: 511/533

Durchflusszytometer-Laserlinien488

Zur Verwendung mit (Anwendung)Durchflusszytometrie

Zur Verwendung mit (Geräte)Attune™ Akustisches Fokussierzytometer, Durchflusszytometer

MarkertypAndere Labels oder Farbstoffe

Marker oder FarbstoffCellEvent™ Caspase 3/7 Nachweisreagenz Grün, SYTOX AADvanced Totzellfarbstoff

ProduktlinieCellEvent

ProdukttypCaspase-Assay-Kit

Menge100 Assays

VersandbedingungNasseis

LöslichkeitDMSO (Dimethylsulfoxid)

NachweisverfahrenFluoreszenz

FormatKit

Unit SizeEach

Inhalt und Lagerung

Enthält 1 Fläschchen CellEvent™ Caspase 3/7 Nachweisreagenz Grün, 1 Fläschchen SYTOX AADvanced Totzellfarbstoff und 1 Fläschchen DMSO.

Alle Fläschchen aufrecht bei oder unter -20 °C lagern, mit Trockenmittel und lichtgeschützt.

Häufig gestellte Fragen (FAQ)

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?

No, it must be applied to live cell populations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I stain cells with nucleic acid stains?

Yes, you may use SYTOX AADvanced Dead Cell Stain that is included in the CellEvent Caspase-3/7 Green Ready Flow Reagent (Cat. No. R37167) and in the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Cat. No. C10427, C10740). You may also try using other nucleic acid stains in the red or far red channels for staining cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I multiplex with fluorescent immunolabeling?

Yes. You can apply surface labels or other reagents. We recommend applying the surface labels or other reagents first.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the CellEvent Caspase-3/7 Green Detection Reagent fixable?

The CellEvent Caspase-3/7 Green Detection Reagent is not covalently attached to any cellular component and may be lost upon fixation. For flow cytometry applications, after applying the reagent, you should examine without washing or fixation. For imaging applications, the CellEvent product signal may be retained after fixation in 3.7% formaldehdye for 15 min.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit, 100 Assays FAQs

Zitierungen und Referenzen (13)

Zitierungen und Referenzen

Abstract

Counteracting autophagy overcomes resistance to everolimus in mantle cell lymphoma.

Authors:Rosich L, Xargay-Torrent S, López-Guerra M, Campo E, Colomer D, Roué G,

Journal:Clin Cancer Res

PubMed ID:22879389

'Mantle cell lymphoma (MCL) is an aggressive B-lymphoid neoplasm with poor response to conventional chemotherapy and short survival. The phosphatidylinositol 3-kinase/Akt/mTOR survival pathway is constitutively activated in MCL cells, thereby making the mTOR inhibition an attractive therapeutic strategy. The first clinical studies of everolimus (RAD001), an mTOR inhibitor, in relapsed ... More

The cytotoxicity of bupivacaine, ropivacaine, and mepivacaine on human chondrocytes and cartilage.

Authors:Breu A, Rosenmeier K, Kujat R, Angele P, Zink W,

Journal:

PubMed ID:23749443

'Intraarticular injections of local anesthetics are frequently used as part of multimodal pain regimens. However, recent data suggest that local anesthetics affect chondrocyte viability. In this study, we assessed the chondrotoxic effects of mepivacaine, ropivacaine, and bupivacaine. We hypothesized that specific cytotoxic potencies directly correlate with analgesic potencies, and that ... More

Proteomic analysis reveals that pardaxin triggers apoptotic signaling pathways in human cervical carcinoma HeLa cells: cross talk among the UPR, c-Jun and ROS.

Authors:Huang TC, Chen JY,

Journal:Carcinogenesis

PubMed ID:23615400

Pardaxin, an antimicrobial peptide secreted by the Red Sea flatfish Pardachirus marmoratus, inhibits proliferation and induces apoptosis of human cancer cell lines. However, the underlying molecular mechanisms are only partially understood at present. In this study, we used proteomic approaches and network reconstruction to clarify the mechanism of pardaxin-induced apoptosis ... More

Salinomycin inhibits breast cancer progression via targeting HIF-1a/VEGF mediated tumor angiogenesis in vitro and in vivo.

Authors:Dewangan J, Srivastava S, Mishra S, Divakar A, Kumar S, Rath SK

Journal:Biochem Pharmacol

PubMed ID:31028743

'Cancer is a complex disease wherein cells begin to divideabnormally and spread into surrounding tissues. Angiogenesis plays a crucial role in tumor progression as it is required for sustained growth and metastasis, therefore targeting angiogenesis is a promising therapeutic approach for breast cancer management. Salinomycin (SAL) has been reported to ... More

Gel-based cell manipulation method for isolation and genotyping of single-adherent cells.

Authors:Negishi R, Iwata R, Tanaka T, Kisailus D, Maeda Y, Matsunaga T, Yoshino T

Journal:Analyst

PubMed ID:30302469

'Genetic analysis of single-cells is widely recognized as a powerful tool for understanding cellular heterogeneity and obtaining genetic information from rare populations. Recently, many kinds of single-cell isolation systems have been developed to facilitate single-cell genetic analysis. However, these systems mainly target non-adherent cells or cells in a cell suspension. ... More

View all CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit, 100 Assays citations