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Zitierungen und Referenzen (382)
Invitrogen™
6-FAM (6-Carboxyfluorescein), single isomer
Das einzelne Isomer 6-FAM enthält eine Carbonsäure, die zur Reaktion mit primären Aminen über die Carbodiimid-Aktivierung der Carbonsäure verwendet werdenWeitere Informationen
| Katalognummer | Menge |
|---|---|
| C1360 | 100 mg |
Katalognummer C1360
Preis (EUR)
317,00
Each
Menge:
100 mg
Preis (EUR)
317,00
Each
Das einzelne Isomer 6-FAM enthält eine Carbonsäure, die zur Reaktion mit primären Aminen über die Carbodiimid-Aktivierung der Carbonsäure verwendet werden kann. Fluorescein ist das häufigste fluoreszierende Derivatisierungsreagenz zur Markierung von Biomolekülen. Neben seiner relativ hohen Absorptivität, der hervorragenden Fluoreszenzquantenausbeute und der guten Wasserlöslichkeit verfügt Fluorescein über ein Erregungsmaximum, das der 488 nm-Spektrallinie des Argon-Ionen-Lasers sehr nahe kommt.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Chemische ReaktivitätAmin
Anregung488
Marker oder FarbstoffFITC (Fluorescein)
Produkttyp6-FAM
Menge100 mg
Reaktiver TeilCarbonsäure
VersandbedingungRaumtemperatur
MarkertypKlassische Farbstoffe
Unit SizeEach
Inhalt und Lagerung
Lagerung bei Raumtemperatur und vor Licht geschützt
Zitierungen und Referenzen (382)
Zitierungen und Referenzen
Abstract
Membrane vesicles containing the Sendai virus binding glycoprotein, but not the viral fusion protein, fuse with phosphatidylserine liposomes at low pH.
Journal:Biochemistry
PubMed ID:3021204
Membrane vesicles containing the Sendai virus hemagglutinin/neuraminidase (HN) glycoprotein were able to induce carboxyfluorescein (CF) release from loaded phosphatidylserine (PS) but not loaded phosphatidylcholine (PC) liposomes. Similarly, fluorescence dequenching was observed only when HN vesicles, bearing self-quenched N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine (N-NBD-PE), were incubated with PS but not PC liposomes. Thus, fusion between
Receptor-mediated endocytosis of antibody-opsonized liposomes by tumor cells.
Journal:Proc Natl Acad Sci U S A
PubMed ID:7001454
'Specific receptor-mediated delivery of the contents of small, sonicated liposomes was studied with three murine tumor cell types: an IgG Fc receptor-negative nonphagocytic line (EL4); an Fc receptor-positive phagocytic line (P388D1); and an Fc receptor-positive nonphagocytic line (P388). The liposomes (formed from phosphatidylcholines, cholesterol, and dinitrophenyl-substituted phosphatidylethanolamine) contained carboxyfluorescein as
Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein.
Journal:Biochemistry
PubMed ID:1510932
'Water rapidly crosses the plasma membranes of red blood cells (RBCs) and renal tubules through highly specialized channels. CHIP28 is an abundant integral membrane protein in RBCs and renal tubules, and Xenopus laevis oocytes injected with CHIP28 RNA exhibit high osmotic water permeability, Pf [Preston et al. (1992) Science 256,
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Luminal pH regulated calcium release kinetics in sarcoplasmic reticulum vesicles.
Journal:Biochemistry
PubMed ID:8873610
'Calcium binding to triads isolated from rabbit skeletal muscle followed a single hyperbolic function in the pH range 5.5-8.0. Maximal binding was obtained at pH 8.0; decreasing the pH decreased the binding capacity and, at pH < or = 6.0, increased Kd 2-fold. These results indicate that lowering the pH