Search

  • Auftragsstatus
  • Eilbestellung
  • Aktionen
CyQUANT™ NF Zellproliferations-Assay
Zitierungen und Referenzen (65)
Invitrogen™

CyQUANT™ NF Zellproliferations-Assay

Der CyQUANT™ NF Zellproliferations-Assay ist eine schnelle und empfindliche Methode zur Zellzählung in einer Population und zur Messung der ProliferationWeitere Informationen
Have Questions?
Ansicht ändernbuttonViewtableView
KatalognummerZelltypMenge
C7026For cells in culture1000 Assays
C35011Direct Cell10 Microplates
C35013Direct Red Cell10 Mikrotiterplatten
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 ml
Katalognummer C7026
Preis (EUR)
685,65
キャンペーン価格
722,00
Ersparnis 36,35 (5%)
Each
Zelltyp:
For cells in culture
Menge:
1000 Assays
Preis (EUR)
685,65
キャンペーン価格
722,00
Ersparnis 36,35 (5%)
Each
Der CyQUANT™ NF Zellproliferations-Assay ist eine schnelle und empfindliche Methode zur Zellzählung in einer Population und zur Messung der Proliferation im Mikrotiterplattenformat.

Merkmale des CyQUANT™ NF Zellproliferations-Assay:

• Empfindlichere Assays als MTT- oder AlamarBlue™ Assays
• Linearer Nachweisbereich von 100 bis 20.000 Zellen pro Well (96-Well Mikrotiterplatte)
•Assays können in einer Stunde abgeschlossen werden

Schnelle und einfache Methode zur Messung der Zellproliferation
Der CyQUANT™ NF Zellproliferations-Assay erfordert keine Zelllyse, lange Inkubationen, Radioaktivität oder Entfernung von Färbemittel aus Zellen. Der CyQUANT™ NF-Assay macht den Einfrier- und Auftauschritt der Zelllyse des ursprünglichen CyQUANT™ Proliferationsassays hinfällig, indem ein zellmembrangängiger, DNA-bindender Farbstoff in Kombination mit einem Permeabilisationsreagenz für die Plasmamembran verwendet wird. Der CyQUANT™ NF Zellproliferationsassay kann im 96 Well- oder 384 Well-Mikrotiterplattenformat verwenden werden und ist in zwei Konfigurationen erhältlich: ein 200 Assay-Kit (C35007) für kleinere Probengrößen und ein 1.000 Assay-Kit (C35006) für Anwendungen mit hohem Durchsatz.

AlamarBlue™ ist ein eingetragenes Warenzeichen von TREK Diagnostic Systems.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
ZellpermeabilitätZellundurchlässig
ZelltypFor cells in culture
BeschreibungCyQUANT™ Zellproliferationsassaykit, für Zellen in der Kultur
NachweisverfahrenFluoreszenz, Fluoreszent
FarbstofftypAndere Etiketten oder Farbstoffe
Anregung/Emission480/520
Format96 Well-Platte, 96-Well Platte
Menge1000 Assays
VersandbedingungRaumtemperatur, Raumtemperatur
FarbeGrün
Emission520 nm
Excitation Wavelength Range480 nm
Zur Verwendung mit (Anwendung)Proliferationsassay
Zur Verwendung mit (Geräte)Mikrotiterplatten-Lesegerät, Mikrotiterplatten-Lesegerät
ProduktlinieCyQUANT
ProdukttypZellproliferationsassay
Unit SizeEach
Inhalt und Lagerung
Enthält CyQUANT™ GR Farbstoff, Zelllysepuffer und Bakteriophagen-λ-DNA.

Häufig gestellte Fragen (FAQ)

Can CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026) be used on organoids in Matrigel medium?

CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) has been validated for use with the AlgiMatrix 3D Culture System, but we cannot guarantee that it will work with other 3D culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026)?

The composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture is proprietary.

Which cell proliferation assays can I use with 3D culture systems?

alamarBlue Cell Viability Reagent, PrestoBlue Cell Viability Reagent, and CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) have been validated for use with the AlgiMatrix 3D Culture System and should also work with other 3D culture systems. For further details, please refer to this poster (https://aacrjournals.org/cancerres/article/70/8_Supplement/3234/563757/Abstract-3234-AlgiMatrixT-3-D-cell-culture-system) and protocol (http://www.thermofisher.com/us/en/home/references/protocols/cell-culture/3-d-cell-culture-protocol/algimatrix-viability-using-cyquant.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (65)

Zitierungen und Referenzen
Abstract
Growth factor-induced proliferation in corneal epithelial cells is mediated by 12(S)-HETE.
Authors:Ottino P,Taheri F,Bazan HE
Journal:Experimental eye research
PubMed ID:12697425
PURPOSE: Previous studies in our laboratory have shown that 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE), a product of 12-lipoxygenase (12-LOX) activity, is the predominant metabolite formed in rabbit corneas after injury. The present study was undertaken to investigate the effects of epidermal growth factor (EGF), hepatocyte growth factor (HGF), and keratinocyte growth factor ... More
Caspase activation contributes to delayed death of heat-stressed striatal neurons.
Authors:White MG, Emery M, Nonner D, Barrett JN
Journal:J Neurochem
PubMed ID:14622126
'Hyperthermia can contribute to brain damage both during development and post-natally. We used rat embryonic striatal neurons in culture to study mechanisms underlying hyperthermia-induced neuronal death. Heat stress at 43 degrees C for 2 h produced no obvious signs of damage during the first 12 h after the stress, but ... More
Endothelial cell surface F1-F0 ATP synthase is active in ATP synthesis and is inhibited by angiostatin.
Authors:Moser TL, Kenan DJ, Ashley TA, Roy JA, Goodman MD, Misra UK, Cheek DJ, Pizzo SV
Journal:Proc Natl Acad Sci U S A
PubMed ID:11381144
'Angiostatin blocks tumor angiogenesis in vivo, almost certainly through its demonstrated ability to block endothelial cell migration and proliferation. Although the mechanism of angiostatin action remains unknown, identification of F(1)-F(O) ATP synthase as the major angiostatin-binding site on the endothelial cell surface suggests that ATP metabolism may play a role ... More
Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.
Authors:Silva E, Gomes P, Soares-da-Silva P,
Journal:J Membr Biol
PubMed ID:17334838
'Na(+)/K(+)-ATPase plays a key role in the transport of Na(+) throughout the nephron, but ageing appears to be accompanied by changes in the regulation and localization of the pump. In the present study, we examined the effect of in vitro cell ageing on the transport of Na(+) and K(+) ions ... More
Identification of the binding site for fibrinogen recognition peptide gamma 383-395 within the alpha(M)I-domain of integrin alpha(M)beta2.
Authors:Yakubenko VP, Solovjov DA, Zhang L, Yee VC, Plow EF, Ugarova TP
Journal:J Biol Chem
PubMed ID:11278633
'The leukocyte integrin alpha(M)beta(2) (Mac-1, CD11b/CD18) is a cell surface adhesion receptor for fibrinogen. The interaction between fibrinogen and alpha(M)beta(2) mediates a range of adhesive reactions during the immune-inflammatory response. The sequence gamma(383)TMKIIPFNRLTIG(395), P2-C, within the gamma-module of the D-domain of fibrinogen, is a recognition site for alpha(M)beta(2) and alpha(X)beta(2). ... More
View all CyQUANT™ NF Zellproliferations-Assay citations