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FluoCells™ Prepared Slide #2 (BPAE cells with Mouse Anti-α-tubulin, AlexaFluor™ 488, FL Goat Anti-Mouse IgG, Texas Red™-X Phalloidin, and DAPI)
Zitierungen und Referenzen (1)
Invitrogen™

FluoCells™ Prepared Slide #2 (BPAE cells with Mouse Anti-α-tubulin, AlexaFluor™ 488, FL Goat Anti-Mouse IgG, Texas Red™-X Phalloidin, and DAPI)

FluoCells Fertigpräparate enthalten mehrfach markierte Zellpräparationen für fluoreszenzmikroskopische Untersuchungen. FluoCells Fertigpräparat Nr. 2 enthält bovine pulmonale arterielle Endothelzellen (BPAEC). F-AktinWeitere Informationen
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KatalognummerMenge
F14781Je 1
Katalognummer F14781
Preis (EUR)
414,00
Each
Menge:
Je 1
Preis (EUR)
414,00
Each
FluoCells Fertigpräparate enthalten mehrfach markierte Zellpräparationen für fluoreszenzmikroskopische Untersuchungen. FluoCells Fertigpräparat Nr. 2 enthält bovine pulmonale arterielle Endothelzellen (BPAEC). F-Aktin ist mit Texas Red-X Phalloidin, Mikrotubuli sind mit dem monoklonalen Anti-Rind-α-Tubulin Maus-Antikörper 236-10501 in Verbindung mit dem BODIPY FL Ziege-Anti-Maus-IgG-Antikörper und der Kern ist mit DAPI markiert.

Das vollständige Angebot an Mikroskop-Kalibrierungsreagenzien anzeigen ›

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Zur Verwendung mit (Anwendung)Immunfluoreszenz
MengeJe 1
VersandbedingungRaumtemperatur
ProduktlinieBODIPY, FluoCells, Texas Red
TypVorbereiteter Mikroskopobjektträger
Unit SizeEach
Inhalt und Lagerung
Bei Raumtemperatur lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?

Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (1)

Zitierungen und Referenzen
Abstract
Practical confocal microscopy and the evaluation of system performance.
Authors:Zucker RM, Price OT
Journal:Methods
PubMed ID:10491274
'The laser scanning confocal microscope has enormous potential in many fields of biology. Currently there is a subjective nature in the assessment of a confocal microscope''s performance by primarily evaluating the system with a specific test slide provided by the user''s laboratory. To achieve better performance from the equipment, it ... More