Phusion™ HF and GC Buffer Packs
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Thermo Scientific™

Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
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KatalognummerChemischer Name oder MaterialFarbe
F538LHF Buffer PackGreen
F518LHF Buffer PackColorless
F519LGC Buffer PackColorless
F520LDetergent-free FF Buffer PackColorless
F521LDetergent-free GC Buffer PackColorless
F539LGC Buffer PackGreen
Katalognummer F538L
Preis (EUR)
33,65
Online Exclusive
35,37
Ersparnis 1,72 (5%)
Each
Zum Warenkorb hinzufügen
Chemischer Name oder Material:
HF Buffer Pack
Farbe:
Green
Preis (EUR)
33,65
Online Exclusive
35,37
Ersparnis 1,72 (5%)
Each
Zum Warenkorb hinzufügen
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications
Chemischer Name oder MaterialHF Buffer Pack
FarbeGreen
EnthältDas Phusion Green HF-Puffer-Pack (F-538L) enthält:
• 5X Phusion Green HF-Puffer (1X Puffer ergibt 1,5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Bei -20 °C lagern.
Zur Verwendung mit (Anwendung)Standard-PCR
Menge4 × 1,5 ml
Unit SizeEach

Häufig gestellte Fragen (FAQ)

Can I use Phusion Green and Phire Green PCR products for subsequent applications such as sequencing, ligation, and restriction digestion?

Yes. The dyes do not interfere with downstream applications such as DNA sequencing, ligation, and restriction digestion.

What dyes are present in Phusion Green and Phire Green buffers?

Phusion Green and Phire Green buffers contain two dyes for monitoring electrophoresis progress. The blue dye migrates with 3-5 kb DNA fragments and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm, respectively.