FluoSpheres™ Carboxylate-Modified Microspheres

Zitierungen und Referenzen (4)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8792
Price: 523.65 EUR

Erzielen Sie die hellste Fluoreszenz mit carboxylatmodifizierten FluoSphere Mikrosphären, die in verschiedenen Farben und Partikelgrößen erhältlich sind.

Ansicht ändern

Katalognummer	Durchmesser (metrisch)	Farbe	Anregung/Emission	Menge
F8792 auch als F-8792 bezeichnet	0,04 μm	Orange	540/560 nm	1 ml
F8803	0,1 μm	Gelbgrün	505/515 nm	10 ml
F8816	1,0 μm	Purpurrot	625/645 nm	2 ml
F8823	1,0 μm	Gelbgrün	505/515 nm	10 ml
F8801	0,1 μm	Rot	580/605 nm	10 ml
F8811	0,2 μm	Gelb-Grün	505/515 nm	10 ml
F8807	0,2 μm	Dunkelrot	660/680 nm	2 ml
F10720	0,04 μm	Gelb-grün, Orange, Rot, Dunkelrot	505/515, 540/560, 580/605, 660/680 nm	1 ml (je)
F20881	0,2 μm	Orange	365/610 nm	2 ml
F8783 auch als F-8783 bezeichnet	0,02 μm	Dunkelrot	660/680 nm	2 ml
F8786	0,02 μm	Rot	580/605 nm	10 ml
F8795 auch als F-8795 bezeichnet	0,04 μm	Gelb-Grün	505/515 nm	1 ml
F8813	0,5 μm	Gelbgrün	505/515 nm	10 ml
F8820	1,0 μm	Orange	540/560 nm	10 ml
F8825	2,0 μm	Nilrot	535/575 nm	2 ml
F8827	2,0 μm	Gelb-Grün	505/515 nm	2 ml
F8781 auch als F-8781 bezeichnet	0,02 μm	Blau	365/415 nm	10 ml
F8782 auch als F-8782 bezeichnet	0,02 μm	Purpurrot	625/645 nm	2 ml
F8784	0,02 μm	Nilrot	535/575 nm	10 ml
F8787	0,02 μm	Gelb-grün	505/515 nm	10 ml
F8789 auch als F-8789 bezeichnet	0,04 μm	Dunkelrot	660/680 nm	1 ml
F8793	0,04 μm	Rot	580/605 nm	1 ml
F8794	0,04 μm	Rot-Orange	565/580 nm	1 ml
F8797	0,1 μm	Blau	350/440 nm	10 ml
F8799	0,1 μm	Infrarot	715/755 nm	1 ml
F8800 auch als F-8800 bezeichnet	0,1 μm	Orange	540/560 nm	10 ml
F8805	0,2 μm	Blau	365/415 nm	10 ml
F8806	0,2 μm	Purpurrot	625/645 nm	2 ml
F8809	0,2 μm	Orange	540/560 nm	10 ml
F8810	0,2 μm	Rot	580/605 nm	10 ml
F8812	0,5 μm	Rot	580/605 nm	10 ml
F8814	1,0 μm	Blau	365/415 nm	10 ml
F8815	1,0 μm	Blau	350/440 nm	10 ml
F8819	1,0 μm	Nilrot	535/575 nm	10 ml
F8821	1,0 μm	Rot	580/605 nm	10 ml
F8824 auch als F-8824 bezeichnet	2,0 μm	Blau	365/415 nm	2 ml
F8826	2,0 μm	Rot	580/605 nm	2 ml

37 Optionen

Katalognummer F8792

auch als F-8792 bezeichnet

Preis (EUR)

523,65

Precio exclusivo en nuestra web

698,00

Ersparnis 174,35 (25%)

Each

Durchmesser (metrisch):

0,04 μm

Farbe:

Orange

Anregung/Emission:

540/560 nm

Menge:

1 ml

Preis (EUR)

523,65

Precio exclusivo en nuestra web

698,00

Ersparnis 174,35 (25%)

Each

Mit unserer umfangreichen Auswahl an FluoSpheres carboxylatmodifizierter Mikrosphären lassen sich Durchflusszytometrie, Mikroskopie, HTS, HCS, Immunassays und andere Laboranwendungen einfach durchführen. FluoSphere Beads sind für die passive Adsorption oder aktive, kovalente Kupplung von Proteinen, Nukleinsäuren und Biomolekülen für Partikelauffanganwendungen einsetzbar. FluoSphere Mikrosphären sind mit proprietären Fluoreszenzfarbstoffen beladen, was sie zu den hellsten Mikrosphären auf dem Markt macht.

Visualisieren Sie die hellste Fluoreszenz für Laboranwendungen wie Fluoreszenzmikroskopie, Durchflusszytometrie, HTS, HCS und Zelltracing mit unseren Carboxylate-Modified FluoSphere Mikrosphären, die aus Polystyrol-Mikrosphären hergestellt und mit verschiedenen proprietären Farbstoffen beladen werden. Mit speziellen Färbemethoden können alle fluoreszierenden Farbstoffmoleküle in jeder Polystyrol-Mikrospäre statt auf der Oberfläche des Beads enthalten sein. Diese Schutzumgebung innerhalb des Beads schützt den Farbstoff vor schädlichen Umwelteinflüssen, wie z. B. Photobleichung. Unsere Carboxylat-modifizierten Mikrosphären sind mit einem hydrophilen Polymer beschichtet, das mehrere Carbonsäuren für die kovalente Bindung von Liganden enthält. Eine Reihe von Partikelgrößen ist für verschiedene Forschungszwecke und Experimente verfügbar.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Anregung/Emission540/560 nm

ProduktlinieFLUOSPHERES

Menge1 ml

OberflächenmodifikationCarboxylat

FarbeOrange

Durchmesser (metrisch)0,04 μm

Zur Verwendung mit (Anwendung)Fluoreszenzmikroskopie

MaterialPolystyrol

ProdukttypCarboxylat-modifizierte Mikrosphäre

Unit SizeEach

Inhalt und Lagerung

Im Kühlschrank (2 bis 8 °C) aufbewahren und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Zitierungen und Referenzen (4)

Zitierungen und Referenzen

Abstract

Viral nanoparticles as tools for intravital vascular imaging.

Authors:Lewis JD, Destito G, Zijlstra A, Gonzalez MJ, Quigley JP, Manchester M, Stuhlmann H

Journal:Nat Med

PubMed ID:16501571

'A significant impediment to the widespread use of noninvasive in vivo vascular imaging techniques is the current lack of suitable intravital imaging probes. We describe here a new strategy to use viral nanoparticles as a platform for the multivalent display of fluorescent dyes to image tissues deep inside living organisms. ... More

Angiotensin II attenuates synaptic GABA release and excites paraventricular-rostral ventrolateral medulla output neurons.

Authors:Li DP, Pan HL

Journal:J Pharmacol Exp Ther

PubMed ID:15681656

'The hypothalamic paraventricular nucleus (PVN) neurons regulate sympathetic outflow through projections to the spinal cord and rostral ventrolateral medulla (RVLM). Although the PVN-RVLM pathway is important for the action of brain angiotensin II (Ang II) on autonomic control, the cellular mechanisms involved are not fully known. In this study, we ... More

In situ fluorescent protein imaging with metal film-enhanced total internal reflection microscopy.

Authors:Burghardt TP, Charlesworth JE, Halstead MF, Tarara JE, Ajtai K

Journal:Biophys J

PubMed ID:16565065

Fluorescence detection of single molecules provides a means to investigate protein dynamics minus ambiguities introduced by ensemble averages of unsynchronized protein movement or of protein movement mimicking a local symmetry. For proteins in a biological assembly, taking advantage of the single molecule approach could require single protein isolation from within ... More

Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.

Authors:Mlodzianoski MJ, Curthoys NM, Gunewardene MS, Carter S, Hess ST

Journal:PLoS One

PubMed ID:27002724

'Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of ... More

FluoSpheres™ Carboxylate-Modified Microspheres

Häufig gestellte Fragen (FAQ)

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Zitierungen und Referenzen (4)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?