FluoSpheres™ Carboxylate-Modified Microspheres

Zitierungen und Referenzen (9)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8810
Price: 720.00 EUR

Erzielen Sie die hellste Fluoreszenz mit carboxylatmodifizierten FluoSphere Mikrosphären, die in verschiedenen Farben und Partikelgrößen erhältlich sind.

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Katalognummer	Durchmesser (metrisch)	Farbe	Anregung/Emission	Menge
F8810	0,2 μm	Rot	580/605 nm	10 ml
F8803	0,1 μm	Gelbgrün	505/515 nm	10 ml
F8816	1,0 μm	Purpurrot	625/645 nm	2 ml
F8823	1,0 μm	Gelbgrün	505/515 nm	10 ml
F8801	0,1 μm	Rot	580/605 nm	10 ml
F8811	0,2 μm	Gelb-Grün	505/515 nm	10 ml
F8807	0,2 μm	Dunkelrot	660/680 nm	2 ml
F10720	0,04 μm	Gelb-grün, Orange, Rot, Dunkelrot	505/515, 540/560, 580/605, 660/680 nm	1 ml (je)
F20881	0,2 μm	Orange	365/610 nm	2 ml
F8783 auch als F-8783 bezeichnet	0,02 μm	Dunkelrot	660/680 nm	2 ml
F8786	0,02 μm	Rot	580/605 nm	10 ml
F8795 auch als F-8795 bezeichnet	0,04 μm	Gelb-Grün	505/515 nm	1 ml
F8813	0,5 μm	Gelbgrün	505/515 nm	10 ml
F8820	1,0 μm	Orange	540/560 nm	10 ml
F8825	2,0 μm	Nilrot	535/575 nm	2 ml
F8827	2,0 μm	Gelb-Grün	505/515 nm	2 ml
F8781 auch als F-8781 bezeichnet	0,02 μm	Blau	365/415 nm	10 ml
F8782 auch als F-8782 bezeichnet	0,02 μm	Purpurrot	625/645 nm	2 ml
F8784	0,02 μm	Nilrot	535/575 nm	10 ml
F8787	0,02 μm	Gelb-grün	505/515 nm	10 ml
F8789 auch als F-8789 bezeichnet	0,04 μm	Dunkelrot	660/680 nm	1 ml
F8792 auch als F-8792 bezeichnet	0,04 μm	Orange	540/560 nm	1 ml
F8793	0,04 μm	Rot	580/605 nm	1 ml
F8794	0,04 μm	Rot-Orange	565/580 nm	1 ml
F8797	0,1 μm	Blau	350/440 nm	10 ml
F8799	0,1 μm	Infrarot	715/755 nm	1 ml
F8800 auch als F-8800 bezeichnet	0,1 μm	Orange	540/560 nm	10 ml
F8805	0,2 μm	Blau	365/415 nm	10 ml
F8806	0,2 μm	Purpurrot	625/645 nm	2 ml
F8809	0,2 μm	Orange	540/560 nm	10 ml
F8812	0,5 μm	Rot	580/605 nm	10 ml
F8814	1,0 μm	Blau	365/415 nm	10 ml
F8815	1,0 μm	Blau	350/440 nm	10 ml
F8819	1,0 μm	Nilrot	535/575 nm	10 ml
F8821	1,0 μm	Rot	580/605 nm	10 ml
F8824 auch als F-8824 bezeichnet	2,0 μm	Blau	365/415 nm	2 ml
F8826	2,0 μm	Rot	580/605 nm	2 ml

37 Optionen

Katalognummer F8810

Preis (EUR)

720,00

Each

Zum Warenkorb hinzufügen

Durchmesser (metrisch):

0,2 μm

Farbe:

Rot

Anregung/Emission:

580/605 nm

Menge:

10 ml

Preis (EUR)

720,00

Each

Zum Warenkorb hinzufügen

Mit unserer umfangreichen Auswahl an FluoSpheres carboxylatmodifizierter Mikrosphären lassen sich Durchflusszytometrie, Mikroskopie, HTS, HCS, Immunassays und andere Laboranwendungen einfach durchführen. FluoSphere Beads sind für die passive Adsorption oder aktive, kovalente Kupplung von Proteinen, Nukleinsäuren und Biomolekülen für Partikelauffanganwendungen einsetzbar. FluoSphere Mikrosphären sind mit proprietären Fluoreszenzfarbstoffen beladen, was sie zu den hellsten Mikrosphären auf dem Markt macht.

Visualisieren Sie die hellste Fluoreszenz für Laboranwendungen wie Fluoreszenzmikroskopie, Durchflusszytometrie, HTS, HCS und Zelltracing mit unseren Carboxylate-Modified FluoSphere Mikrosphären, die aus Polystyrol-Mikrosphären hergestellt und mit verschiedenen proprietären Farbstoffen beladen werden. Mit speziellen Färbemethoden können alle fluoreszierenden Farbstoffmoleküle in jeder Polystyrol-Mikrospäre statt auf der Oberfläche des Beads enthalten sein. Diese Schutzumgebung innerhalb des Beads schützt den Farbstoff vor schädlichen Umwelteinflüssen, wie z. B. Photobleichung. Unsere Carboxylat-modifizierten Mikrosphären sind mit einem hydrophilen Polymer beschichtet, das mehrere Carbonsäuren für die kovalente Bindung von Liganden enthält. Eine Reihe von Partikelgrößen ist für verschiedene Forschungszwecke und Experimente verfügbar.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Anregung/Emission580/605 nm

ProduktlinieFLUOSPHERES

Menge10 ml

OberflächenmodifikationCarboxylat

FarbeRot

Durchmesser (metrisch)0,2 μm

Zur Verwendung mit (Anwendung)Fluoreszenzmikroskopie

MaterialPolystyrol

ProdukttypCarboxylat-modifizierte Mikrosphäre

Unit SizeEach

Inhalt und Lagerung

Im Kühlschrank (2 °C bis 8 °C) aufbewahren und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Zitierungen und Referenzen (9)

Zitierungen und Referenzen

Abstract

Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion.

Authors:Mullock BM, Smith CW, Ihrke G, Bright NA, Lindsay M, Parkinson EJ, Brooks DA, Parton RG, James DE, Luzio JP, Piper RC

Journal:Mol Biol Cell

PubMed ID:10982406

'Protein traffic from the cell surface or the trans-Golgi network reaches the lysosome via a series of endosomal compartments. One of the last steps in the endocytic pathway is the fusion of late endosomes with lysosomes. This process has been reconstituted in vitro and has been shown to require NSF, ... More

Estimated functional diameter of alveolar septal microvessels in zone 1.

Authors:Conhaim RL, Rodenkirch LA

Journal:Am J Physiol

PubMed ID:8853334

'To estimate the functional diameter of alveolar septal microvessels in zone 1, we perfused isolated rat lungs with fluorescent latex particles of specific diameters (0.24, 0.49, 1.05, or 4.0 microns) at pulmonary artery pressures (Ppulmart) that were either 5 or 10 cmH2O less than the air inflation pressure (Pinflat, 25 ... More

A murine scavenger receptor MARCO recognizes polystyrene nanoparticles.

Authors:Kanno S, Furuyama A, Hirano S,

Journal:Toxicol Sci

PubMed ID:17361018

'Recent toxicological studies indicate that nanoparticles or ultrafine particles (< 100 nm) are more toxic than fine particles (< 2 microm) because of their greater surface area. It is well known that alveolar macrophages play an important role in the first defense against various environmental particles and microorganisms. This is ... More

Ehrlichia chaffeensis infections in Drosophila melanogaster.

Authors:Luce-Fedrow A, Von Ohlen T, Chapes SK,

Journal:Infect Immun

PubMed ID:19687202

Ehrlichia chaffeensis is an obligate, intracellular bacterium, transmitted by the tick Amblyomma americanum, and is the causative agent of human monocytic ehrlichiosis infections. We previously demonstrated that E. chaffeensis is capable of growing in Drosophila S2 cells. Therefore, we tested the hypothesis that E. chaffeensis can infect adult Drosophila melanogaster. ... More

Enhanced viscoelasticity of human cystic fibrotic sputum correlates with increasing microheterogeneity in particle transport.

Authors:Dawson M, Wirtz D, Hanes J,

Journal:J Biol Chem

PubMed ID:13679362

Current biochemical characterizations of cystic fibrosis (CF) sputum do not address the high degree of microheterogeneity in the rheological properties of the mucosal matrix and only provide bulk-average particle diffusion coefficients. The viscoelasticity of CF sputum greatly reduces the diffusion rates of colloidal particles, limiting the effectiveness of gene delivery ... More

View all FluoSpheres™ Carboxylate-Modified Microspheres citations

FluoSpheres™ Carboxylate-Modified Microspheres

Häufig gestellte Fragen (FAQ)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Zitierungen und Referenzen (9)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?