IBlot™ Transferstapel, Nitrozellulose, reguläre Größe
The original iBlot Transfer Stack SKUs will be discontinued effective July 1, 2026 or until inventory is depleted. The latest generation iBlot 3 Dry Blotting System is the recommended replacement and enables twice the throughput and features optional built-in cooling for better consistency. Learn more about the benefits of the iBlot 3 here.
IBlot™ Transferstapel, Nitrozellulose, reguläre Größe
Invitrogen™

IBlot™ Transferstapel, Nitrozellulose, reguläre Größe

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Die iBlot™ Transferstapel werden verwendet, um Proteine mit dem iBlot™ Gel-Transfergerät zu übertragen. Die iBlot™ Transferstapel sind für den EinmalgebrauchWeitere Informationen
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KatalognummerMengeDimensions (LxW)
IB30100110 SetsNormal, 13,5 x 8 cm
IB3010313 x 10/SatzNormal, 13,5 x 8 cm
Katalognummer IB301001
Preis (EUR)
316,00
Each
Zum Warenkorb hinzufügen
Menge:
10 Sets
Dimensions (LxW):
Normal, 13,5 x 8 cm
Preis (EUR)
316,00
Each
Zum Warenkorb hinzufügen
Die iBlot™ Transferstapel werden verwendet, um Proteine mit dem iBlot™ Gel-Transfergerät zu übertragen. Die iBlot™ Transferstapel sind für den Einmalgebrauch vorgesehen und in drei Varianten für unterschiedliche Anwendungen erhältlich: iBlot™ Gel-Transferstapel verfügen über integrierte PVDF- oder Nitrozellulose-Transfermembranen für Dry-Blotting von Proteinen, iBlot™ DNA-Transferstapel verfügen über eine integrierte Nylon-Transfermembran für Dry-Blotting von DNA und iBlot™ Western Detektionsstapel werden verwendet, um den Western Nachweis auf zuvor geblotteten Nitrozellulose- oder PVDF-Membranen durchzuführen. Jeder iBlot™ Transfer Stack verfügt über eine Kupferelektrode und entsprechende Kathoden- und Anoden-Puffer in der Gelmatrix für den schnellen, zuverlässigen Transfer von Proteinen und DNA.

Nitrozellulosemembran
Die Nitrozellulosemembran (0,2 µm Porengröße), die in diesem Stapel enthalten ist, besteht aus 100 % reiner Nitrozellulose zur Gewährleistung eines hochwertigen Transfers. Die Membran ist kompatibel mit gängigen Nachweismethoden, z. B. Färbung, Strukturanalyse, Fluoreszenz oder Radiomarkierung. Die Proteine binden aufgrund von hydrophoben und elektrostatischen Wechselwirkungen an die Membran. Das Protein-Bindungsvermögen beträgt 209 µg/cm2.

Transferstapel-Größen
Die normale Größe (13 cm x 8,3 cm) wird für alle Novex™ Midi-Gel-Typen (oder gleichwertig) und E-PAGE™ 48- oder 96-Gele verwendet. Die geringe Größe (8 cm x 8 cm) wird für alle Novex™ Mini-Gele (oder gleichwertig) verwendet.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Umweltfreundliche EigenschaftenNachhaltige Entsorgung
Menge10 Sets
VersandbedingungRaumtemperatur
Dimensions (LxW)Normal, 13,5 x 8 cm
FormatTransferstapel
Länge (metrisch)13,5 cm
MaterialCellulosenitrat
Porengröße0.2 μm
ProduktlinieiBlot
Ausreichend für10 Blots
Breite (metrisch)8 cm
Unit SizeEach
Inhalt und Lagerung
10 Transferstapel-Sets in 1 Box. Bei Raumtemperatur lagern.

Häufig gestellte Fragen (FAQ)

Following the release of the iBlot 2 Gel Transfer Device, are consumables for the original iBlot Gel Transfer Device still available?

Yes, all original iBlot stacks are still available for purchase. You can find them in the original iBlot Gel Transfer Device Reagents and Resources (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/iblot-dry-blotting-system/reagents-resources-original-iblot-gel-transfer-device.html). However, please note that they are not compatible with the iBlot 2 Gel transfer Device.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the iBlot 2 Gel Transfer Device compatible with original iBlot Transfer Stacks?

No. Do not use iBlot Transfer Stacks in the iBlot 2 Gel Transfer Device, and do not mix components between iBlot Transfer Stacks and iBlot 2 Transfer Stacks.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I get better transfer of high-molecular weight proteins?

Proteins larger than ~150 kDa migrate more slowly than smaller proteins. Therefore, more time is required to transfer them from gel to membrane. We recommend extending the transfer time by 8 to 10 minutes for optimal transfer of proteins >150 kDa using the iBlot Dry Blotting System.

To enhance transfer efficiency, we also recommend adding an equilibration (gel-soaking) step between electrophoresis and western transfer and using NuPAGE Invitrogen 3 - 8% Tris-acetate gels for electrophoresis. We have an application note available, titled “Transferring Large and Small Proteins Using the iBlot Dry Blotting System”. To download the PDF, see the iBlot Reagents Resources page. (http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/western-blotting/western-blot-transfer/iblot-dry-blotting-system/original-iblot-resources.html)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Occasionally my western blots have high background. What do you recommend?

This may be a result of insufficient blocking or nonspecific binding. We suggest trying our WesternBreeze Blocker/Diluent (Cat. No. WB7050). We have been using it with good success. Additionally, you should optimize primary and secondary antibody concentrations as generally recommended for any new blotting technique. Many cases of high background can be resolved by further diluting one or both antibody preparations.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is it possible to substitute the membrane from the iBlot Transfer Stack with my specialized membrane?

In theory, you can replace the membrane provided in your iBlot Transfer Stack with any membrane that is compatible with western blotting. To do this, cut the alternative membrane to match the size of your gel, and wet the membrane. Then, either place the alternative membrane on top of the integrated membrane, or carefully remove the integrated membrane from the gel matrix with forceps and replace it with the new membrane. Note that we only support the use of iBlot Transfer Stacks when they are used with the provided instructions.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all IBlot™ Transferstapel, Nitrozellulose, reguläre Größe FAQs