For Western blot washing.TRIS-buffered saline (TBS, 10X) is used as a washing buffer for electrodes, alkaline phosphatase and peroxidase conjugates in dot blot assay. Further, it acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Anwendungen
Für das Waschen beim Western Blotting. TRIS-gepufferte Kochsalzlösung (TBS, 10X) wird als Waschpuffer für Elektroden, alkalische Phosphatase- und Peroxidase-Konjugate in Dot-Blot-Assays verwendet. Darüber hinaus fungiert sie als pH-Stabilisator für Waschen ohne Unterbrechung von Antikörper-Antigen-Bindungsinteraktionen.
Löslichkeit
Mit Wasser mischbar.
Hinweise
500 mM Tris, 1.5 M NaCl, pH 7.4. Kühl lagern. Nicht kompatibel mit starken Oxidationsmitteln.
RUO – Research Use Only
Allgemeine Referenzen:
- Anastasia, A.; Barker, P. A.; Chao, M. V.; Hempstead, B. L. Detection of p75NTR Trimers: Implications for Receptor Stoichiometry and Activation. J. Neurosci. 2015, 35 (34), 11911-11920.
- Brand, C. S.; Sadana, R.; Malik, S.; Smrcka, A. V.; Dessauer, C. W. Adenylyl Cyclase 5 Regulation by G βγ Involves Isoform-Specific Use of Multiple Interaction Sites. Mol. Pharmacol. 2015, 88 (4), 758-767.