LysoTracker™ Red DND-99 ist ein rot fluoreszierender Farbstoff zur Markierung und zum Tracking saurer Organellen in lebenden Zellen.Zu den MerkmalenWeitere Informationen
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Katalognummer
Menge
L7528
20 x 50 μL
Katalognummer L7528
Preis (EUR)
638,65
Exklusiv online
672,00
Ersparnis 33,35 (5%)
Each
Menge:
20 x 50 μL
Preis (EUR)
638,65
Exklusiv online
672,00
Ersparnis 33,35 (5%)
Each
LysoTracker™ Red DND-99 ist ein rot fluoreszierender Farbstoff zur Markierung und zum Tracking saurer Organellen in lebenden Zellen.
Zu den Merkmalen aller LysoTracker™ Sonden gehören:
• Hohe Selektivität für saure Organellen • Gute Retention nach der Aldehydfixierung • Effektive Markierung von lebenden Zellen bei nanomolaren Konzentrationen • Live-Zellbildgebung • Anregung/Emission: 577/590 nm
Einfaches, hochspezifisches einstufiges Färben und Tracking von sauren Organellen Die LysoTracker™ Sonden bestehen aus einem Fluorophor, der mit einer schwachen Base verbunden ist, die nur teilweise bei neutralem pH-Wert protoniert wird. So können LysoTracker™ Sonden Zellmembranen frei durchdringen, sodass sie lebende Zellen markieren können. LysoTracker Sonden™ sind sehr selektiv für saure Organellen, und im Gegensatz zu Dinitrophenyl (DNP) benötigen LysoTracker™ Sonden keinen zweiten Antikörper für den Nachweis.
LysoTracker Sonden™ sind in einer Vielzahl von Fluoreszenzfarben erhältlich.
Testosterone signaling through internalizable surface receptors in androgen receptor-free macrophages.
Authors:Benten WP,Lieberherr M,Stamm O,Wrehlke C,Guo Z,Wunderlich F
Journal:Molecular biology of the cell
PubMed ID:10512854
Testosterone acts on cells through intracellular transcription-regulating androgen receptors (ARs). Here, we show that mouse IC-21 macrophages lack the classical AR yet exhibit specific nongenomic responses to testosterone. These manifest themselves as testosterone-induced rapid increase in intracellular free [Ca(2+)], which is due to release of Ca(2+) from intracellular Ca(2+) stores. ... More
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PubMed ID:9030618
Biocompatibility, endocytosis, and intracellular trafficking of mesoporous silica and polystyrene nanoparticles in ovarian cancer cells: effects of size and surface charge groups.
Authors:Ekkapongpisit M, Giovia A, Follo C, Caputo G, Isidoro C,
Journal:Int J Nanomedicine
PubMed ID:22904626
Nanoparticles engineered to carry both a chemotherapeutic drug and a sensitive imaging probe are valid tools for early detection of cancer cells and to monitor the cytotoxic effects of anticancer treatment simultaneously. Here we report on the effect of size (10-30 nm versus 50 nm), type of material (mesoporous silica ... More
Induction of autophagy and cell death by tamoxifen in cultured retinal pigment epithelial and photoreceptor cells.
Authors:Cho KS, Yoon YH, Choi JA, Lee SJ, Koh JY,
Journal:Invest Ophthalmol Vis Sci
PubMed ID:22786900
We investigated the mechanism of tamoxifen (TAM) retinotoxicity using human retinal pigment epithelial (RPE)-derived (ARPE-19) and photoreceptor-derived (661W) cells. Cultured ARPE-19 and 661W cells were treated with 5 to 10 µM TAM, and the resultant cell death was quantified using lactate dehydrogenase (LDH) release assay. Cellular oxidative stress was determined ... More
Streptococcal serum opacity factor increases the rate of hepatocyte uptake of human plasma high-density lipoprotein cholesterol.
Authors:Gillard BK, Rosales C, Pillai BK, Lin HY, Courtney HS, Pownall HJ,
Journal:Biochemistry
PubMed ID:20879789
Serum opacity factor (SOF), a virulence determinant of Streptococcus pyogenes, converts plasma high-density lipoproteins (HDL) to three distinct species: lipid-free apolipoprotein (apo) A-I, neo HDL, a small discoidal HDL-like particle, and a large cholesteryl ester-rich microemulsion (CERM) that contains the cholesterol esters (CE) of up to ~400000 HDL particles and ... More