MANT-GDP (2'-(or-3')-<i>O</i>-(<i>N</i>-Methylanthraniloyl) Guanosine 5'-Diphosphate, Disodium Salt)

Zitierungen und Referenzen (51)

Invitrogen™

MANT-GDP (2'-(or-3')-O-(N-Methylanthraniloyl) Guanosine 5'-Diphosphate, Disodium Salt)

Das Nukleotidanalogon MANT-GDP ist am Ribose-Anteil modifiziert. Die kompakte Reife des MANT Fluorophors und seine Anbindeposition führt zu Nukleotid-Analoga, dieWeitere Informationen

Katalognummer	Menge
M12414	400 μl

Katalognummer M12414

Preis (EUR)

674,00

Menge:

400 μl

Preis (EUR)

674,00

Das Nukleotidanalogon MANT-GDP ist am Ribose-Anteil modifiziert. Die kompakte Reife des MANT Fluorophors und seine Anbindeposition führt zu Nukleotid-Analoga, die eine minimale Störung von Nukleotid-Protein-Interaktionen induzieren. Da die MANT-Fluoreszenz empfindlich auf die Umgebung des Fluorophors reagiert, können Nukleotid-Protein-Interaktionen direkt nachweisbar sein. MANT Nukleotide sind wertvolle Sonden der Struktur und enzymatischen Aktivität von Nukleotid-Bindungsproteinen.

Nur für Forschungszwecke. Darf nicht für diagnostische Verfahren eingesetzt werden.

Specifications

MarkierungsmethodeChemische Markierung, direkte Markierung

Marker oder FarbstoffMANT (N-Methylanthraniloyl)

ProdukttypMANT-GDP

Menge400 μl

VersandbedingungNasses Eis, Nasseis

Konzentration5 mM

Unit SizeEach

Inhalt und Lagerung

In einem Tiefkühlgerät (-5 bis -30 °C) lagern und vor Licht schützen.

Zitierungen und Referenzen (51)

Zitierungen und Referenzen

Abstract

Authors:

Journal:

PubMed ID:11063593

The role of Mg2+ cofactor in the guanine nucleotide exchange and GTP hydrolysis reactions of Rho family GTP-binding proteins.

Authors:Zhang B, Zhang Y, Wang Z, Zheng Y

Journal:J Biol Chem

PubMed ID:10843989

'The biological activities of Rho family GTPases are controlled by their guanine nucleotide binding states in cells. Here we have investigated the role of Mg(2+) cofactor in the guanine nucleotide binding and hydrolysis processes of the Rho family members, Cdc42, Rac1, and RhoA. Differing from Ras and Rab proteins, which ... More

Kinetics of Cdc42 membrane extraction by Rho-GDI monitored by real-time fluorescence resonance energy transfer.

Authors:Nomanbhoy TK, Erickson JW, Cerione RA

Journal:Biochemistry

PubMed ID:10026253

'The mechanisms underlying the ability of the Rho-GDP dissociation inhibitor (RhoGDI) to elicit the release of Rho-related GTP-binding proteins from membranes is currently unknown. In this report, we have set out to address this issue by using fluorescence resonance energy transfer approaches to examine the functional interactions of the RhoGDI ... More

Biochemical analysis of SopE from Salmonella typhimurium, a highly efficient guanosine nucleotide exchange factor for RhoGTPases.

Authors:Rudolph MG, Weise C, Mirold S, Hillenbrand B, Bader B, Wittinghofer A, Hardt WD

Journal:J Biol Chem

PubMed ID:10521431

'RhoGTPases are key regulators of eukaryotic cell physiology. The bacterial enteropathogen Salmonella typhimurium modulates host cell physiology by translocating specific toxins into the cytoplasm of host cells that induce responses such as apoptotic cell death in macrophages, the production of proinflammatory cytokines, the rearrangement of the host cell actin cytoskeleton ... More

Alanine scan mutagenesis of the switch I domain of the Caulobacter crescentus CgtA protein reveals critical amino acids required for in vivo function.

Authors:Lin B, Skidmore JM, Bhatt A, Pfeffer SM, Pawloski L, Maddock JR

Journal:Mol Microbiol

PubMed ID:11251813

'The Caulobacter crescentus CgtA protein is a member of the Obg/GTP1 subfamily of monomeric GTP-binding proteins. In vitro, CgtA displays moderate affinity for both GDP and GTP and displays rapid exchange rate constants for either nucleotide, indicating that the guanine nucleotide-binding and exchange properties of CgtA are different from those ... More

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